{"title":"PERFORMANCE OF PETRIFILM™ KIT-HEC FOR ENUMERATION OF ESCHERICHIA COLI O157:H7 IN ARTIFICIALLY CONTAMINATED GROUND BEEF","authors":"SUSANA M. I. SAAD, BERNADETTE D.G.M. FRANCO","doi":"10.1111/j.1745-4581.1999.tb00387.x","DOIUrl":null,"url":null,"abstract":"<p><b>Abstract </b> <i>Petrifilm™ Kit-HEC (3M Company, St. Paul, MN) was used to monitor the growth of</i> Escherichia coli <i>O157:H7 in ground beef co-inoculated with microorganisms of the background flora, including nonpathogenic</i> E.coli, Pseudomonas putida <i>or</i> Leuconostoc <i>sp. The growth was monitored in beef samples stored at room temperature and under refrigeration. A cultural procedure using sorbitol MacConkey agar was used for comparison. The correlation between counts of</i> E.coli <i>O157:H7 using these procedures was high: 99.1% for samples kept at room temperature and 94.9% for those kept under refrigeration.</i></p>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"7 3","pages":"173-181"},"PeriodicalIF":0.0000,"publicationDate":"2011-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.1999.tb00387.x","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.1999.tb00387.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract Petrifilm™ Kit-HEC (3M Company, St. Paul, MN) was used to monitor the growth of Escherichia coli O157:H7 in ground beef co-inoculated with microorganisms of the background flora, including nonpathogenic E.coli, Pseudomonas putida or Leuconostoc sp. The growth was monitored in beef samples stored at room temperature and under refrigeration. A cultural procedure using sorbitol MacConkey agar was used for comparison. The correlation between counts of E.coli O157:H7 using these procedures was high: 99.1% for samples kept at room temperature and 94.9% for those kept under refrigeration.