Sequencing, Not Culture, Proved Fast Way To Find New Hot Spring Virus

C. Potera
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Abstract

Culture-independent methods are making it possible to discover and partly characterize viruses and their hosts straight from hot springs such as those in Yellowstone National Park (YNP)—short-circuiting the traditional approach that depends on first growing samples in vitro, according to Rebecca Hochstein at Montana State University (MSU) in Bozeman and her collaborators. In this way, they discovered the Acidianus tailed spindle virus (ATSV), which infects archaeal species found in the Crater Hills Thermal Basin of YNP. Details appeared 13 January 2016 in the Journal of Virology (doi:10.1128/JVI.03098–15).
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测序而非培养被证明是发现新的温泉病毒的快速方法
波兹曼蒙大拿州立大学(MSU)的丽贝卡·霍克斯坦(Rebecca Hochstein)和她的合作者说,不依赖培养的方法使直接从黄石国家公园(YNP)的温泉中发现和部分表征病毒及其宿主成为可能,这使依赖于首先在体外培养样本的传统方法变得简单。通过这种方式,他们发现了酸尾梭形病毒(ATSV),它感染了YNP火山口山热盆地中发现的古细菌物种。详情发表在2016年1月13日的《病毒学杂志》(doi:10.1128/ JVI.03098-15)。
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