The Later Calcification of the Bacterial Molds in the Human Dental Calculus Formed by the Extracellular Calcification

T. Kodaka, M. Yamada
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引用次数: 5

Abstract

ln the bacterial molds of human dental calculus formed by the extracellular calcification, whitlockite and octacalcium phosphate crystals as well as apatite crystals were occasionally precipitated in the later stages of calculus formation . Thus, the deposits would be similar to the calculus formed by both the intraand extracellular calcifications . Key werds: human dental calculus, extracellular calcification , bacterial molds, later calcification, scanning electron microscopy As is well known, there exist the intraand extracellular calcifications of oral microorganisms in human dental calculus'-5) . The crystals are biological apatites including hydroxyapatite , Cadeficient and carbonate apatites'-8), although oral microorganisms were occasionally replaced by Mg-containing whitlockite crystals9-11) . The formation processes will be roughly divided into 3 cases : I to III1-541,12); case I: the intraand the extracellular calcifications occur at almost the same time, case II: the intracellular calcification first occurs followed by the extracellular calcification, and case III: the extracellular calcification first occurs. In case III, the bacterial molds will be retained during the time of the calculus formation , although the deposits of case III would become like a calculus of case I in the later formation stage. In this study, crystals deposited within the bacterial molds were investigated with scanning electron microscopy (SEM). Materials and Methods Supraand subgingival dental calculi attached to human teeth, which had been used in our previous studies, 9-11,13,14) were also used in these observations. The fractured calculus were treated with 10% sodium hypochlorite (Na0C1) for 1 hour in order to remove organic debris9-'2, 14,15). This was followed by rinsing in running water for 1 hour and drying in the air. The samples were observed with a Hitachi S-430 SEM at 20 kV accelerating voltage after coating with a 10 to 15-nm platinum-palladium layer .
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细胞外钙化形成的人牙结石细菌霉菌的后期钙化
在细胞外钙化形成的人牙石细菌霉菌中,牙石形成后期偶有白介石、磷酸钙八晶及磷灰石结晶析出。因此,沉积物类似于细胞内和细胞外钙化形成的结石。【关键词】人牙石,细胞外钙化,细菌霉菌,后期钙化,扫描电镜众所周知,人牙石中存在口腔微生物的细胞内和细胞外钙化。这些晶体是生物磷灰石,包括羟基磷灰石、缺钙磷灰石和碳酸盐磷灰石,尽管口腔微生物偶尔会被含镁的白介石晶体所取代(11)。形成过程将大致分为3种情况:I至iii - 51,12);病例1:细胞内和细胞外钙化几乎同时发生,病例2:细胞内钙化先发生,其次是细胞外钙化,病例3:细胞外钙化先发生。在情况III中,在结石形成期间细菌霉菌将被保留,尽管情况III的沉积物在形成后期会变得像情况I的结石。在本研究中,用扫描电子显微镜(SEM)研究了沉积在细菌霉菌内的晶体。材料和方法我们也使用了附着在人类牙齿上的龈上和龈下牙结石,这在我们之前的研究中已经使用过(9-11,13,14)。骨折结石用10%次氯酸钠(Na0C1)处理1小时,以去除有机碎屑[1,2,14,15]。然后用流水冲洗1小时,晾干。在20 kV加速电压下,用日立S-430扫描电镜对样品进行观察。
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