MLV-derived retroviral pseudotype vectors

Abstract

One of the strategies used to alter the tropism of retroviral vectors involves the substitution of their envelope glycoproteins with those of other viruses. This results in the generation of pseudotype vector particles exhibiting properties that may be advantageous for gene transfer into certain human cells. Moreover, when the envelope glycoproteins from simian and human immunodeficiency viruses were used to pseudotype Murine Leukemia Virus (MLV), the resulting vectors mediated selective gene transfer into defined human cell types. Enhanced transduction efficacy and cell targeting via retroviral pseudotype vectors may be important prerequisites for future gene therapy applications.