Diversity of Mirabilis jalapa L. based on isozymic band of peroxidase

Suhar Irianto, S. Sutarno, A. Setyawan
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引用次数: 1

Abstract

Mirabilis jalapa L. is a gardening plant which growth easily planted because their beautiful color and flower’s ornaments. The aims of the research were to find out the variation of isozyme band of peroxidase (PER) and their close relationship of M. jalapa cultivated based on their different flower’s color. The method used in this study was polyacrilamide gel electrophoresis. The collecting data were analyzed using calculation of association coefficient (Gower’s coefficient) with cluster analysis used for similarities of their arithmetic. The results showed that in M. jalapa were found 21 variation of isozyme bands of peroxidase (PER) from 8 Rf of 0.04; 0.08; 0.20; 0.28; 0.36; 0.48; 0,60; and 0.64. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) have the nearest genetic relationship with similarity index of 0.76. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) joined with red flower’s (MER) on 0.73 of similarity index then joined with white flower’s (PUT) on 0.64 of similarity index and yellow flower’s (KUN) on 0.49 of similarity index. At least, M. jalapa with orange flower’s (ORA) joined with M. jalapa cultivars on 0.41 of similarity index.
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基于过氧化物酶同工酶带的紫茉莉多样性研究
Mirabilis jalapa L.是一种易于种植的园艺植物,因为它们美丽的颜色和花朵的装饰。本研究的目的是根据不同花色的紫堇,探讨其过氧化物酶(PER)同工酶带的变异及其亲缘关系。本研究采用聚丙烯酰胺凝胶电泳方法。采用关联系数(gower系数)计算方法对收集的数据进行分析,并采用聚类分析方法对其算法的相似性进行分析。结果表明,在8个Rf值为0.04的位点上,发现了21个过氧化物酶同工酶条带的变异;0.08;0.20;0.28;0.36;0.48;0, 60;和0.64。白、红结花€™s (BIN)和白、红线花€™s (GAR)的亲缘关系最接近,相似指数为0.76。白红结花€™s (BIN)和白红结花€™s (GAR)与红结花€™s (MER)的相似性指数为0.73,与白花€™s (PUT)的相似性指数为0.64,与黄花€™s (KUN)的相似性指数为0.49。至少,橙花紫茉莉(ORA)与紫茉莉品种的相似性指数为0.41。
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