Fitriningrum R, Sugiyarto, Susilowati A. 2013. Analysis of carbohydrate content of various maturity levels of Mountain Papaya (Carica pubescens) fruit from Kejajar and Sembungan Villages, Dieng Plateau, Central Java. Bioteknologi 10: 6-14. Mountain papayas (Carica pubescens Lenne & K. Koch) at Dieng Plateau, Central Java were distributed narrowly (+1400 m.asl until +2400 m.asl). Different altitude could determine the climatic environment factors as well as plant metabolism processes. The aim of this research was to compare the carbohydrates content on various maturity levels of mountain papaya fruit from Kejajar and Sembungan Village, Dieng Plateau. Samples of mountain papaya fruit on various maturity levels i.e: upper part, middle part and lower part, were taken from Kejajar Village (+1400 m.asl) and Sembungan Village (+2400 m.asl). Each sample was analyzed it’s rough fibre part, reducing sugar and total of carbohydrate from it’s seed salute and pericarp. The data was analyzed descriptive-comparatively. Result showed that there was tendency more mature of fruit, it’s total carbohydrates content increased, except the total carbohydrate content of pericarp of mountain papaya fruit from Sembungan Village. More mature of mountain papaya fruit, it’s rough fibre part decreased. Highest of reducing sugar was observed on middle fruit. The total carbohydrates content of mountain papaya from Kejajar Village were higher than from Sembungan Village.
刘建军,刘建军,刘建军。2013。中爪哇Dieng高原Kejajar村和Sembungan村不同成熟度山木瓜果实碳水化合物含量分析。生物技术,10:6 -14。中爪哇Dieng高原山地木瓜(Carica pubescens Lenne & K. Koch)分布狭窄(+1400 ~ +2400 m.asl)。不同海拔可以决定气候环境因子和植物代谢过程。本研究的目的是比较滇高原克加扎尔村和Sembungan村不同成熟度的山木瓜果实的碳水化合物含量。在Kejajar村(+1400 m.asl)和Sembungan村(+2400 m.asl)采集了不同成熟度的山木瓜果实样品,即上部、中部和下部。对每个样品的粗纤维部分、还原糖和总碳水化合物进行分析。对数据进行描述性比较分析。结果表明:除三丰干村山木瓜果皮总碳水化合物含量外,果实有越成熟的趋势,果实总碳水化合物含量越高;越成熟的山木瓜果实,其粗纤维部分越少。还原糖含量最高的是中果。山木瓜的总碳水化合物含量高于山木瓜的总碳水化合物含量。
{"title":"Analisis kandungan karbohidrat pada berbagai tingkat kematangan buah karika (Carica pubescens) di Kejajar dan Sembungan, Dataran Tinggi Dieng, Jawa Tengah","authors":"R. Fitriningrum, S. Sugiyarto, Ari Susilowati","doi":"10.13057/BIOTEK/C100102","DOIUrl":"https://doi.org/10.13057/BIOTEK/C100102","url":null,"abstract":"Fitriningrum R, Sugiyarto, Susilowati A. 2013. Analysis of carbohydrate content of various maturity levels of Mountain Papaya (Carica pubescens) fruit from Kejajar and Sembungan Villages, Dieng Plateau, Central Java. Bioteknologi 10: 6-14. Mountain papayas (Carica pubescens Lenne & K. Koch) at Dieng Plateau, Central Java were distributed narrowly (+1400 m.asl until +2400 m.asl). Different altitude could determine the climatic environment factors as well as plant metabolism processes. The aim of this research was to compare the carbohydrates content on various maturity levels of mountain papaya fruit from Kejajar and Sembungan Village, Dieng Plateau. Samples of mountain papaya fruit on various maturity levels i.e: upper part, middle part and lower part, were taken from Kejajar Village (+1400 m.asl) and Sembungan Village (+2400 m.asl). Each sample was analyzed it’s rough fibre part, reducing sugar and total of carbohydrate from it’s seed salute and pericarp. The data was analyzed descriptive-comparatively. Result showed that there was tendency more mature of fruit, it’s total carbohydrates content increased, except the total carbohydrate content of pericarp of mountain papaya fruit from Sembungan Village. More mature of mountain papaya fruit, it’s rough fibre part decreased. Highest of reducing sugar was observed on middle fruit. The total carbohydrates content of mountain papaya from Kejajar Village were higher than from Sembungan Village.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"10 1","pages":"6-14"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Widiastuti A, Sobir, Suhartanto MR. 2010. Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics. Bioteknologi 7: 85-98. The aim of this research was to increase genetic variability of mangosteen (Garcinia mangostana L.) irradiated by gamma rays dosage of 0 Gy, 20 Gy, 25 Gy, 30 Gy,35 Gy and 40 Gy. Plant materials used were seeds collected from Cegal Sub-village, Karacak Village, Leuwiliang Sub-district, Bogor District, West Java. Data was generated from morphological and anatomical characteristics. The result indicated that increasing of gamma ray dosage had inhibited ability of seed to growth, which needed longer time and decreased seed viability. Morphologically, it also decreased plant heigh, stem diameter, leaf seizure, and amount of leaf. Anatomically, stomatal density had positive correlation with plant height by correlation was 90% and 74%. Gamma rays irradiation successfully increase morphological variability until 30%. Seed creavage after irradiation increased variability and survival rate of mangosteen.
{"title":"Analisis keragaman manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan karakteristik morfologi dan anatomi","authors":"Alfin Widiastuti, Sobir Sobir, M. R. Suhartanto","doi":"10.13057/BIOTEK/C070203","DOIUrl":"https://doi.org/10.13057/BIOTEK/C070203","url":null,"abstract":"Widiastuti A, Sobir, Suhartanto MR. 2010. Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics. Bioteknologi 7: 85-98. The aim of this research was to increase genetic variability of mangosteen (Garcinia mangostana L.) irradiated by gamma rays dosage of 0 Gy, 20 Gy, 25 Gy, 30 Gy,35 Gy and 40 Gy. Plant materials used were seeds collected from Cegal Sub-village, Karacak Village, Leuwiliang Sub-district, Bogor District, West Java. Data was generated from morphological and anatomical characteristics. The result indicated that increasing of gamma ray dosage had inhibited ability of seed to growth, which needed longer time and decreased seed viability. Morphologically, it also decreased plant heigh, stem diameter, leaf seizure, and amount of leaf. Anatomically, stomatal density had positive correlation with plant height by correlation was 90% and 74%. Gamma rays irradiation successfully increase morphological variability until 30%. Seed creavage after irradiation increased variability and survival rate of mangosteen.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"7 1","pages":"23-33"},"PeriodicalIF":0.0,"publicationDate":"2010-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harini M, Astirin OP. 2009. Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Bioteknologi 6: 55-62. This study aims to determine treatment effect of VCO on blood cholesterol levels in hypercholesterolemic white rat (Rattus norvegicus L.). This study used 25 male rats of Wistar strain divided into five treatment groups, namely: control, simvastatin (1.3 mL/270 g BW), cholesterol (9:1 lard), VCO 1 (1 mL/270 g BW), and VCO 2 (1.3 mL/270 g BW). Treatment was given orally. Total cholesterol, LDL and HDL cholesterol levels were measured at day 1, day 14 and day 28. Cholesterol data (total cholesterol, LDL and HDL) were analyzed by Ancova and followed by contrast test at significance level of 5%.. The results showed that treatment of VCO at different doses significantly affected the decrease in blood total cholesterol, blood LDL levels, increasing blood HDL in hipercholesterolemic white rat.
王志强,黄志强,黄志强。2009。VCO治疗后高胆固醇血症大鼠(褐家鼠)血胆固醇水平。生物技术6:55-62。本研究旨在探讨VCO对高胆固醇血症大鼠(Rattus norvegicus L.)血胆固醇水平的治疗作用。本研究选用Wistar品系雄性大鼠25只,分为对照组、辛伐他汀(1.3 mL/270 g BW)、胆固醇(9:1猪油)、VCO 1 (1 mL/270 g BW)、VCO 2 (1.3 mL/270 g BW) 5个治疗组。给予口服治疗。在第1天、第14天和第28天测定总胆固醇、低密度脂蛋白和高密度脂蛋白胆固醇水平。胆固醇数据(总胆固醇、低密度脂蛋白和高密度脂蛋白)采用Ancova分析,然后在5%显著性水平下进行对比检验。结果表明,不同剂量的VCO处理显著影响了高胆固醇血症大鼠血液总胆固醇、血液LDL水平的降低和血液HDL水平的升高。
{"title":"Kadar kolesterol darah tikus putih (Rattus norvegicus) hiperkolesterolemik setelah perlakuan VCO","authors":"M. Harini, Okid Parama Astirin","doi":"10.13057/BIOTEK/C060204","DOIUrl":"https://doi.org/10.13057/BIOTEK/C060204","url":null,"abstract":"Harini M, Astirin OP. 2009. Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Bioteknologi 6: 55-62. This study aims to determine treatment effect of VCO on blood cholesterol levels in hypercholesterolemic white rat (Rattus norvegicus L.). This study used 25 male rats of Wistar strain divided into five treatment groups, namely: control, simvastatin (1.3 mL/270 g BW), cholesterol (9:1 lard), VCO 1 (1 mL/270 g BW), and VCO 2 (1.3 mL/270 g BW). Treatment was given orally. Total cholesterol, LDL and HDL cholesterol levels were measured at day 1, day 14 and day 28. Cholesterol data (total cholesterol, LDL and HDL) were analyzed by Ancova and followed by contrast test at significance level of 5%.. The results showed that treatment of VCO at different doses significantly affected the decrease in blood total cholesterol, blood LDL levels, increasing blood HDL in hipercholesterolemic white rat.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"6 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2009-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An effort to fulfill nutrient elements on chilly crops can be done through fertilization. The fullfilment of nutrient element by giving organic and chemical fertilizers or the combination of both; either in the form of solid or liqufied fertilizers, on chilly crops can result as physiological response in the form of biomass, chlorophill and nitrogen content on chilly leaves. This research aims to know the influence of various feirtilizing on biomass, chlorophyll and nitrogen content on chilly leaves 'sakti' and 'fantastic'. The research is done in Gatak village, Karangnongko subdistrict, Klaten regency; from September 2006 till March 2007. The research design is factorial design using complete factorial design using complete block of 2 (two ) factors that is chilly varieties and fertilizing treatment. Chilly varieties used in this research are big chilly of 'fantastic' variety and curly chilly of 'sakti' variety. Meanwhile the fertilizing treatment used in this research include without using fertilizer (P 1); Using organic fertilizer 2 kilograms/each plant (P 2); Using organic fertilizer 1 kilogram/each plant + chemical fertilizer (ZA, SP-36, K Cl.= 2:1:1) + pour down NPK Mutiara (P 3); and Organic fertilizer 1 Kilogram/each plant + Chemical Fertilizer (SP 36 : K Cl = 1 : 1) + pour down Liquid Organic Fertilizer Bathari Sri (P 4). When harvest comes (the wet and the dried weight and the fruit crops of each plant ), the chlorophyll content are measured.The measurement of the wet plant uses a pair of scales. The measurement of chlorophyll content is based on Harborne method (1987), while the nitrogen content measurement uses Kjeldal method. Data Analyzes Technique uses Variant Analyzis followed by DMRT test (Duncan's Multi Range Test). The research output shows that various fertilizing treatment has influenced on the wet and dried weight and wet crops weight of each 'Fantastic' chilly plant. Organic + Chemical fertilizer treatment (SP- 36 : K Cl = 1 : 1) + pouring down POC Bathari Sri resulted the best weight of dried plant and the crops weight. Various fertilizing treatment influences on the increases chlorophyll content a of 'Fantastic' chilly; but it does not influence on chlorophyll content b and the total chlorophyll. The highest chlorophyll content a can be seen on the fertilizing uses organic + chemical fertilizer (ZA : SP-36 : K Cl = 2:1:1) + pouring down NPK Mutiara. Various fertilizing treament which influences on the plant wet weight does not influence the dried weight and the crops wet weight for each plant.Fertilizing treatment uses organic + chemical fertilizer (SP- 36 : K Cl = 1 : 1) + pouring down POC 'Bathari Sri' has the best weight of wet plant and fruit. Various fertilizing treatments influence on th increase of chlorophyll content a and total chlorophyll of chilly plant 'Sakti', does not influence on the chlorophyll content b. The highest chloropile content a and the total chloropill can be s
可以通过施肥来补充寒冷作物的营养元素。通过施用有机和化学肥料或两者结合来补充营养元素;无论是固体肥料还是液态肥料,对寒冷作物都能产生生理反应,表现为寒冷叶片上的生物量、叶绿素和氮含量。本研究旨在了解不同施肥方式对寒性叶片‘sakti’和‘fantastic’生物量、叶绿素和氮含量的影响。研究在Klaten摄政Karangnongko街道Gatak村进行;从2006年9月至2007年3月。本研究设计为全因子设计,采用寒性品种和施肥处理2个因子的全因子设计。本研究使用的辣椒品种是“梦幻”品种的大辣椒和“sakti”品种的卷辣椒。同时,本研究采用的施肥处理包括不施肥(P < 1);每株施用有机肥2公斤(P 2);每株使用有机肥1公斤+化肥(ZA、SP-36、K - Cl)。= 2:1:1) +倒NPK Mutiara (p3);和有机肥1公斤/株+化肥(SP 36: K Cl = 1:1) +倒液体有机肥Bathari Sri (p4)。当收获时(每株的湿重、干重和果实作物),测量叶绿素含量。湿植物的测量用一副天平。叶绿素含量的测量采用Harborne法(1987),氮含量的测量采用Kjeldal法。数据分析技术使用变异分析和DMRT检验(邓肯多范围检验)。研究结果表明,不同施肥处理对‘奇妙’寒凉植株的干湿重和湿粒重均有影响。有机+化肥处理(SP- 36: K - Cl = 1:1) +泼施POC可获得最佳干株重和作物重。不同施肥处理对‘奇妙’寒菜叶绿素含量a增加的影响但对叶绿素含量b和总叶绿素含量没有影响。叶绿素含量a在施用有机+化肥(ZA: SP-36: K Cl = 2:1:1) +倒施氮磷钾Mutiara时最高。影响植株湿重的各种施肥处理对单株干重和作物湿重没有影响。施肥处理采用有机+化学肥料(SP- 36: K Cl = 1:1) +浇灌POC 'Bathari Sri'具有最佳的湿植物和果实重量。不同施肥处理对寒性植物‘萨克提’叶绿素含量a和总叶绿素含量的增加有影响,但对叶绿素含量b没有影响。叶绿素含量a和总叶绿素含量在有机+化肥(ZA: SP- 36: K Cl = 2:1:1) +灌施氮磷钾Mutiara的施肥处理中最高。不同施肥处理对‘奇妙’和‘萨克提’冷衣叶片总氮含量的增加没有影响。有机+化肥(ZA: SP-36: K Cl = 2:1:1) +倾泻NPK Mutiara处理与有机+化肥(SP- 36: K Cl = 1:1) +倾泻POC Bathari Sri处理在生物量、叶绿素和氮含量方面没有显著差异。此外,有机+化肥(SP- 36: K Cl = 1)处理:1) +倒POC Bathari Sri从三个方面来看条件较好。研究人员建议使用有机肥+化肥(sp36: kcl = 1:1) +倒POC Bathari Sri的配方施肥,作为种植寒冷的新选择,既经济又环保。关键词:生物量,叶绿素含量,氮含量,寒性作物
{"title":"Biomassa, kandungan klorofil dan nitrogen daun dua varietas cabai (capsium annum L) pada berbagai perlakuan pemupukan","authors":"Suharja","doi":"10.13057/BIOTEK/C060102","DOIUrl":"https://doi.org/10.13057/BIOTEK/C060102","url":null,"abstract":"An effort to fulfill nutrient elements on chilly crops can be done through \u0000fertilization. The fullfilment of nutrient element by giving organic and \u0000chemical fertilizers or the combination of both; either in the form of solid or liqufied fertilizers, on chilly crops can result as physiological response in the form \u0000of biomass, chlorophill and nitrogen content on chilly leaves. This research aims \u0000to know the influence of various feirtilizing on biomass, chlorophyll and nitrogen \u0000content on chilly leaves 'sakti' and 'fantastic'. The research is done in Gatak village, Karangnongko subdistrict, Klaten \u0000regency; from September 2006 till March 2007. The research design is factorial \u0000design using complete factorial design using complete block of 2 (two ) factors \u0000that is chilly varieties and fertilizing treatment. Chilly varieties used in this \u0000research are big chilly of 'fantastic' variety and curly chilly of 'sakti' variety. \u0000Meanwhile the fertilizing treatment used in this research include without using \u0000fertilizer (P 1); Using organic fertilizer 2 kilograms/each plant (P 2); Using organic \u0000fertilizer 1 kilogram/each plant + chemical fertilizer (ZA, SP-36, K Cl.= 2:1:1) + \u0000pour down NPK Mutiara (P 3); and Organic fertilizer 1 Kilogram/each plant + \u0000Chemical Fertilizer (SP 36 : K Cl = 1 : 1) + pour down Liquid Organic Fertilizer \u0000Bathari Sri (P 4). When harvest comes (the wet and the dried weight and the fruit \u0000crops of each plant ), the chlorophyll content are measured.The measurement of \u0000the wet plant uses a pair of scales. The measurement of chlorophyll content is \u0000based on Harborne method (1987), while the nitrogen content measurement uses \u0000Kjeldal method. Data Analyzes Technique uses Variant Analyzis followed by \u0000DMRT test (Duncan's Multi Range Test). The research output shows that various \u0000fertilizing treatment has influenced on the wet and dried weight and wet crops \u0000weight of each 'Fantastic' chilly plant. Organic + Chemical fertilizer treatment (SP- \u000036 : K Cl = 1 : 1) + pouring down POC Bathari Sri resulted the best weight of \u0000dried plant and the crops weight. Various fertilizing treatment influences on the \u0000increases chlorophyll content a of 'Fantastic' chilly; but it does not influence on \u0000chlorophyll content b and the total chlorophyll. The highest chlorophyll content a \u0000can be seen on the fertilizing uses organic + chemical fertilizer (ZA : SP-36 : K Cl \u0000= 2:1:1) + pouring down NPK Mutiara. Various fertilizing treament which \u0000influences on the plant wet weight does not influence the dried weight and the \u0000crops wet weight for each plant.Fertilizing treatment uses organic + chemical \u0000fertilizer (SP- 36 : K Cl = 1 : 1) + pouring down POC 'Bathari Sri' has the best \u0000weight of wet plant and fruit. \u0000Various fertilizing treatments influence on th increase of chlorophyll \u0000content a and total chlorophyll of chilly plant 'Sakti', does not influence on the \u0000chlorophyll content b. The highest chloropile content a and the total chloropill can \u0000be s","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"6 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2009-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agustinus Kuntjoro, S. Sutarno, Okid Parama Astirin
Kuntjoro A, Sutarno, Astirin OP. 2009. Body weight and statistic vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof. Bioteknologi 6: 29- 39. This research is aimed to observe the body weight and statistic vital measurement of 50 Texel sheep. Sheep are classified into five treatments of giving woof P0 (giving tree greenish woof without concentrate), P1 (giving greenish woof and concentrate without adding the ramie hay/0%) concentrate), P2 (giving greenish woof and concentrate by adding 10%) ramie hay), P3 (giving greenish woof and concentrate by adding 20%) ramie hay), P4 (giving greenish woof and concentrate by adding 30%) ramie hay), every treatment was repeated 10 times. The result shows that even it can’t yet replace the concentrate function, but adding ramie hay as much as 10%), 20%) and 30%) on sheep woof can increase the body weight’s growth respectively 186.67 g/day, 153.34 g/day dan 103.34 g/day. The addition of ramie hay 10%), 20%) and 30%) can increase the addition of statistic vital’s measurement on breast of sheep livestock 1.20 cm); 0.95 cm) and 0.90 cm); the addition of statistic vital measurement on the body length of sheep livestock 0.05 cm); 1.00 cm) and 0.75 cm) and also the addition of breast width is 1.50 cm); 0.15 cm) and 0.3 cm). Meanwhile the addition of ramie hay on livestock woof can only increase the addition of statistic vital mesurement on breast at giving 30%) as big as 0.15 cm). It is needed to know further on giving ramie hay by concentration comparasion of hay of different leaf and stem.
{"title":"Bobot badan dan statistik vital domba Texel di Kabupaten Wonosobo dengan pemberian limbah rami sebagai pakan tambahan","authors":"Agustinus Kuntjoro, S. Sutarno, Okid Parama Astirin","doi":"10.13057/BIOTEK/C060104","DOIUrl":"https://doi.org/10.13057/BIOTEK/C060104","url":null,"abstract":"Kuntjoro A, Sutarno, Astirin OP. 2009. Body weight and statistic vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof. Bioteknologi 6: 29- 39. This research is aimed to observe the body weight and statistic vital measurement of 50 Texel sheep. Sheep are classified into five treatments of giving woof P0 (giving tree greenish woof without concentrate), P1 (giving greenish woof and concentrate without adding the ramie hay/0%) concentrate), P2 (giving greenish woof and concentrate by adding 10%) ramie hay), P3 (giving greenish woof and concentrate by adding 20%) ramie hay), P4 (giving greenish woof and concentrate by adding 30%) ramie hay), every treatment was repeated 10 times. The result shows that even it can’t yet replace the concentrate function, but adding ramie hay as much as 10%), 20%) and 30%) on sheep woof can increase the body weight’s growth respectively 186.67 g/day, 153.34 g/day dan 103.34 g/day. The addition of ramie hay 10%), 20%) and 30%) can increase the addition of statistic vital’s measurement on breast of sheep livestock 1.20 cm); 0.95 cm) and 0.90 cm); the addition of statistic vital measurement on the body length of sheep livestock 0.05 cm); 1.00 cm) and 0.75 cm) and also the addition of breast width is 1.50 cm); 0.15 cm) and 0.3 cm). Meanwhile the addition of ramie hay on livestock woof can only increase the addition of statistic vital mesurement on breast at giving 30%) as big as 0.15 cm). It is needed to know further on giving ramie hay by concentration comparasion of hay of different leaf and stem.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"6 1","pages":"23-30"},"PeriodicalIF":0.0,"publicationDate":"2009-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pemanfaatan Limbah Tahu untuk Peningkatan Hasil Tanaman Petsai (Brassica chinensis)","authors":"Yuliadi Asmoro, Suranto, D. Sutoyo","doi":"10.13057/biotek/c050202","DOIUrl":"https://doi.org/10.13057/biotek/c050202","url":null,"abstract":"","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"5 1","pages":"51-55"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pertumbuhan, Kandungan Klorofil, dan Laju Respirasi Tanaman Garut (Maranta arundinacea L.) setelah Pemberian Asam Giberelat (GA3)","authors":"Sugiyarto, Solichatun, Giyatmi Wahyu Lestari","doi":"10.13057/biotek/c050101","DOIUrl":"https://doi.org/10.13057/biotek/c050101","url":null,"abstract":"","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"5 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2008-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mirabilis jalapa L. is a gardening plant which growth easily planted because their beautiful color and flower’s ornaments. The aims of the research were to find out the variation of isozyme band of peroxidase (PER) and their close relationship of M. jalapa cultivated based on their different flower’s color. The method used in this study was polyacrilamide gel electrophoresis. The collecting data were analyzed using calculation of association coefficient (Gower’s coefficient) with cluster analysis used for similarities of their arithmetic. The results showed that in M. jalapa were found 21 variation of isozyme bands of peroxidase (PER) from 8 Rf of 0.04; 0.08; 0.20; 0.28; 0.36; 0.48; 0,60; and 0.64. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) have the nearest genetic relationship with similarity index of 0.76. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) joined with red flower’s (MER) on 0.73 of similarity index then joined with white flower’s (PUT) on 0.64 of similarity index and yellow flower’s (KUN) on 0.49 of similarity index. At least, M. jalapa with orange flower’s (ORA) joined with M. jalapa cultivars on 0.41 of similarity index.
{"title":"Diversity of Mirabilis jalapa L. based on isozymic band of peroxidase","authors":"Suhar Irianto, S. Sutarno, A. Setyawan","doi":"10.13057/BIOTEK/C040101","DOIUrl":"https://doi.org/10.13057/BIOTEK/C040101","url":null,"abstract":"Mirabilis jalapa L. is a gardening plant which growth easily planted because their beautiful color and flower’s ornaments. The aims of the research were to find out the variation of isozyme band of peroxidase (PER) and their close relationship of M. jalapa cultivated based on their different flower’s color. The method used in this study was polyacrilamide gel electrophoresis. The collecting data were analyzed using calculation of association coefficient (Gower’s coefficient) with cluster analysis used for similarities of their arithmetic. The results showed that in M. jalapa were found 21 variation of isozyme bands of peroxidase (PER) from 8 Rf of 0.04; 0.08; 0.20; 0.28; 0.36; 0.48; 0,60; and 0.64. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) have the nearest genetic relationship with similarity index of 0.76. M. jalapa with white and red nodes flower’s (BIN) and white and red line flower’s (GAR) joined with red flower’s (MER) on 0.73 of similarity index then joined with white flower’s (PUT) on 0.64 of similarity index and yellow flower’s (KUN) on 0.49 of similarity index. At least, M. jalapa with orange flower’s (ORA) joined with M. jalapa cultivars on 0.41 of similarity index.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"4 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2007-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRAK Hanifiasti Winedar. 2005. DAYA CERNA PROTEIN PAKAN, KANDUNGAN PROTEIN DAGING DAN PERTAMBAHAN BERAT BADAN AYAM BROILER SETELAH PEMBERIAN PAKAN YANG DIFERMENTASI DENGAN EFFECTIVE MICROORGANISMS-4 (EM-4). FMIPA. UNS. Effective microorganisms-4 (EM-4) merupakan kultur campuran yang terdiri dari bakteri fotosintetik, bakteri asam laktat (Lactobacillus sp), khamir (Saccharomyces sp) Actinomycetes dan jamur fermentasi (Aspergillus sp dan Penicillium sp). Pakan yang terfermentasi EM-4 dapat meningkatkan kapasitas daya cerna melalui perbaikan keseimbangan mikroorganisme dalam saluran pernafasan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan yang difermentasi dengan EM-4 pada berbagai konsentrasi terhadap daya cerna protein pakan, kandungan protein daging dan pertambahan berat badan ayam broiler. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL), lima perlakuan dengan lima ulangan. Perlakuan yang diberikan adalah penambahan larutan starter 5% (P1), 10% (P2), 15% (P3) dan 20% (P4) dan satu kelompok kontrol tanpa penambahan larutan starter (P0). Ayam Broiler yang digunakan adalah ayam broiler jantan sebanyak 25 yang diproduksi oleh CP 707 PT Charoen Pokphand Jaya Farma. Pengukuran kadar protein dilakukan dengan menggunakan metode Kjedahl. Data yang diperoleh dianalisis menggunakan ANAVA dan dilanjutkan dengan uji DMRT dengan taraf signifikansi 5 %. Hasil penelitian yang diperoleh menunjukkan bahwa perlakuan memberikan pengaruh nyata terhadap peningkatan daya cerna protein pakan, kandungan protein daging, dan pertambahan berat badan ayam broiler. Penggunaan EM-4, terutama pada P3 dengan konsentrasi 15 % menyebabkan peningkatan kualitas pakan dan efisiensi penggunaan pakan dengan meningkatnya kandungan protein pada pakan, sehingga dapat meningkatkan daya cerna protein pakan, kandungan protein daging dan pertambahan berat badan ayam broiler. Kata Kunci : Effective Microorganisms-4 (EM-4), daya cerna protein, protein daging, pertambahan berat badan, ayam broiler.
{"title":"DAYA CERNA PROTEIN PAKAN, KANDUNGAN PROTEIN DAGING DAN PERTAMBAHAN BERAT BADAN AYAM BROILER SETELAH PEMBERIAN PAKAN YANG DIFERMENTASI DENGAN EFFECTIVE MICROORGANISMS-4 (EM-4)","authors":"Hanifiasti Winedar","doi":"10.13057/biotek/c030103","DOIUrl":"https://doi.org/10.13057/biotek/c030103","url":null,"abstract":"ABSTRAK \u0000 \u0000 \u0000Hanifiasti Winedar. 2005. DAYA CERNA PROTEIN PAKAN, KANDUNGAN PROTEIN DAGING DAN PERTAMBAHAN BERAT BADAN AYAM BROILER SETELAH PEMBERIAN PAKAN YANG DIFERMENTASI DENGAN EFFECTIVE MICROORGANISMS-4 (EM-4). FMIPA. UNS. \u0000 Effective microorganisms-4 (EM-4) merupakan kultur campuran yang terdiri dari bakteri fotosintetik, bakteri asam laktat (Lactobacillus sp), khamir (Saccharomyces sp) Actinomycetes dan jamur fermentasi (Aspergillus sp dan Penicillium sp). Pakan yang terfermentasi EM-4 dapat meningkatkan kapasitas daya cerna melalui perbaikan keseimbangan mikroorganisme dalam saluran pernafasan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan yang difermentasi dengan EM-4 pada berbagai konsentrasi terhadap daya cerna protein pakan, kandungan protein daging dan pertambahan berat badan ayam broiler. \u0000 Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL), lima perlakuan dengan lima ulangan. Perlakuan yang diberikan adalah penambahan larutan starter 5% (P1), 10% (P2), 15% (P3) dan 20% (P4) dan satu kelompok kontrol tanpa penambahan larutan starter (P0). Ayam Broiler yang digunakan adalah ayam broiler jantan sebanyak 25 yang diproduksi oleh CP 707 PT Charoen Pokphand Jaya Farma. Pengukuran kadar protein dilakukan dengan menggunakan metode Kjedahl. Data yang diperoleh dianalisis menggunakan ANAVA dan dilanjutkan dengan uji DMRT dengan taraf signifikansi 5 %. \u0000 Hasil penelitian yang diperoleh menunjukkan bahwa perlakuan memberikan pengaruh nyata terhadap peningkatan daya cerna protein pakan, kandungan protein daging, dan pertambahan berat badan ayam broiler. Penggunaan EM-4, terutama pada P3 dengan konsentrasi 15 % menyebabkan peningkatan kualitas pakan dan efisiensi penggunaan pakan dengan meningkatnya kandungan protein pada pakan, sehingga dapat meningkatkan daya cerna protein pakan, kandungan protein daging dan pertambahan berat badan ayam broiler. \u0000 \u0000 \u0000 \u0000Kata Kunci : Effective Microorganisms-4 (EM-4), daya cerna protein, protein daging, pertambahan berat badan, ayam broiler.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"3 1","pages":"14-19"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leucaena leucocephala was one of Mimosaceae family which had high protein content nearly the same value as in Soya beans protein content. L. leucocephala could be used as the substitution of Soya beans in production of soy sauce. Soy sauce was one of fermentation liquid products which had flavor and aroma as meat, and had high nutrition value. The aims of the research were (i) to analyze nutrition value in seeds, koji and moromi including carbohydrate, proteins and lipids and (ii) to know the potency of L. leucocephala as raw material in production of soy sauce by fermentation method with Aspergillus oryzae as inoculums. Fermentation was one of soy sauce production processes that involved two steps: (i) the solid stage fermentation and (ii) the brine fermentation. L. leucocephala was fermented with A. oryzae for 3-5 days to produce koji. Koji was soaked in salt solution 20% (1:5 b/v) then it was fermented for 30 days to produce moromi. Nutritional values in L. leucocephala seeds, koji, and moromi was analyzed, i.e. carbohydrate (sugar reduction and starch), protein, and lipids. Moromi filtrate was added by spices to Leucaena sauce. Finally, preferable Leucaena sauce was tested including flavor, aroma, and color. Preferable data was analyzed by non-parametric statistic; it was Friedman Test and followed Wilcoxon Ranking Method. The result showed that value of reducing sugar in L. leucocephala seeds, koji and moromi were 78.38 mg/g; 119.08 mg/g; and 164.29 mg/g. Starch value of seeds, koji and moromi were 274.36 mg/g; 260.92 mg/g and 179.50 mg/g. The value of dissolved protein in seeds, koji and moromi were 107.44 mg/g; 86.1 mg/g; and 208.56 mg/g. The value of lipids on seeds, koji and moromi was 158.87 mg/g; 51.35 mg/g; and 80.86 mg/g. The data of preferable test on Leucaena sauce’s taste was the same as ABC sauce but the aroma of Leucaena sauce had the lowest score than the others. More over, the four kinds of sauce were not different significantly in color. L. leucocephala had potency as raw material in production of soy sauce by fermentation method with A. oryzae as inoculum. Keywords: Leucaena leucocephala, soy sauce, Aspergillus oryzae.
{"title":"Analisis Karbohidrat, Protein, dan Lemak pada Pembuatan Kecap Lamtoro Gung (Leucaena leucocephala) terfermentasi Aspergillus oryzae","authors":"A. Rahayu, Suranto, Tjahjadi Purwoko","doi":"10.13057/biotek/c020103","DOIUrl":"https://doi.org/10.13057/biotek/c020103","url":null,"abstract":"Leucaena leucocephala was one of Mimosaceae family which had high \u0000protein content nearly the same value as in Soya beans protein content. L. \u0000leucocephala could be used as the substitution of Soya beans in production \u0000of soy sauce. Soy sauce was one of fermentation liquid products which had \u0000flavor and aroma as meat, and had high nutrition value. The aims of the \u0000research were (i) to analyze nutrition value in seeds, koji and moromi \u0000including carbohydrate, proteins and lipids and (ii) to know the potency of \u0000L. leucocephala as raw material in production of soy sauce by fermentation \u0000method with Aspergillus oryzae as inoculums. Fermentation was one of soy \u0000sauce production processes that involved two steps: (i) the solid stage \u0000fermentation and (ii) the brine fermentation. L. leucocephala was fermented \u0000with A. oryzae for 3-5 days to produce koji. Koji was soaked in salt solution \u000020% (1:5 b/v) then it was fermented for 30 days to produce moromi. \u0000Nutritional values in L. leucocephala seeds, koji, and moromi was analyzed, \u0000i.e. carbohydrate (sugar reduction and starch), protein, and lipids. Moromi \u0000filtrate was added by spices to Leucaena sauce. Finally, preferable Leucaena \u0000sauce was tested including flavor, aroma, and color. Preferable data was \u0000analyzed by non-parametric statistic; it was Friedman Test and followed \u0000Wilcoxon Ranking Method. The result showed that value of reducing sugar \u0000in L. leucocephala seeds, koji and moromi were 78.38 mg/g; 119.08 mg/g; and \u0000164.29 mg/g. Starch value of seeds, koji and moromi were 274.36 mg/g; \u0000260.92 mg/g and 179.50 mg/g. The value of dissolved protein in seeds, koji \u0000and moromi were 107.44 mg/g; 86.1 mg/g; and 208.56 mg/g. The value of \u0000lipids on seeds, koji and moromi was 158.87 mg/g; 51.35 mg/g; and 80.86 \u0000mg/g. The data of preferable test on Leucaena sauce’s taste was the same as \u0000ABC sauce but the aroma of Leucaena sauce had the lowest score than the \u0000others. More over, the four kinds of sauce were not different significantly \u0000in color. L. leucocephala had potency as raw material in production of soy \u0000sauce by fermentation method with A. oryzae as inoculum. \u0000Keywords: Leucaena leucocephala, soy sauce, Aspergillus oryzae.","PeriodicalId":30431,"journal":{"name":"Bioteknologi","volume":"2 1","pages":"14-20"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66127538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}