Isolation of temperature-sensitive mutants of measles virus.

Yukio Yamazi, F. L. Black, E. Watari
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引用次数: 10

Abstract

Measles virus differs from other members of paramyxoviruses in its prolonged growth cycle1), its degree of involvement of the cell nucleus2), its lack of neuraminidase3) and the sedimentability of its RNA4). We have undertaken a study of mutants of this virus in the hope that the study might circumvent some of the problems encountered with temperaturesensitive (ts) mutants of other paramyxoviruses and cast light on novel features peculiar to measles5•`7). In this paper we report the isolation of 8 ts mutants of measles virus and their biological characterization. (Portions of this work were presented at the 3rd International Congress for Virology, Spain, 19758).) Seligman and Rapp's "giant cell" strain of measles virus9) which is derived from the Edmonston isolate, was used. Vero cells10) grown as stationary monolayer cultures were employed throughout. Eagle's minimal essential medium11) added with 10% calf serum, 100 IU penicillin, 100ƒÊg streptomycin per ml and doubled vitamin concentration was used with Hanks' base for initial seeding and with Earle's salts for maintenance. Virus titrations were
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麻疹病毒温度敏感突变体的分离。
麻疹病毒与其他副粘病毒成员的不同之处在于其生长周期延长、参与细胞核的程度、缺乏神经氨酸酶和rna可沉积性。我们已经对这种病毒的突变体进行了研究,希望这项研究可以规避其他副粘病毒的温度敏感突变体所遇到的一些问题,并阐明麻疹特有的新特征。本文报道了麻疹病毒8个ts突变体的分离及其生物学特性。(这项工作的部分内容已于19758年在西班牙举行的第三届国际病毒学大会上发表)。他们使用了塞利格曼和拉普从埃德蒙斯顿分离出的麻疹病毒的“巨细胞”菌株。Vero细胞作为固定的单层培养物被全程使用。Eagle的最低必需培养基添加10%的小牛血清、100 IU的青霉素、100ƒÊg链霉素每毫升和双倍浓度的维生素,与汉克斯碱一起用于初始播种,与厄尔盐一起用于维持。病毒滴定
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Isolation of temperature-sensitive mutants of measles virus. [Inhibition of nuclear protein synthesis in arbovirus infected cells]. [Effects of heat-labile toxin of B. pertussis on mouse blood cells: the determination of LPF (leukocytosis-promoting factor)]. [Yeast cell cycle and induction rates of respirtion-deficient mutants by 4-nitroquinoline 1-oxide in synchronous cell culture]. [Influence of endotoxin on lymphocytosis].
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