K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama
{"title":"Determination of D-Penicillamine in Serum by High-Performance Liquid Chromatography Using N -[4-(6-Dimethylamino-2-benzofuranyl) phenyl] maleimide","authors":"K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama","doi":"10.14921/JSCC1971B.17.2_51","DOIUrl":null,"url":null,"abstract":"A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Clinical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14921/JSCC1971B.17.2_51","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 3
Abstract
A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.
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