Phylogeny of Dengue Virus 2 based upon the NS3 Gene among USA, Thailand, Singapore, Japan and Philippine

Abstract

Dengue virus is an arbovirus belonging to family flaviviridae causes dengue fever (DF) and dengue hemorrhagic fever (DHF). Dengue virus circulates in most tropical and subtropical regions or the world with 50–100 million human cases annually. Dengue viruses have four known serotypes designated as DENV-1, DENV-2, DENV-3, and DENV-4, with each serotype can cause full spectrum of sign and symptoms associated with DF and DHF. Despite the threat of the dengue virus studies of nucleotide divergence among the different serotypes has largely been limited to a single gene. This lack of basic knowledge of viral diversity severely limits vaccine and anti-viral therapy development efforts. Previously core and pre membrane genes of Dengue have been used for phylogenetic analysis. We used NS3 gene of dengue virus, which is a conserved region of the dengue virus 2 to study the phylogeny of dengue among Philippine, Vietnam, Thailand, Japan, China, Singapore, Sri Lanka and Taiwan. Phylogenetic Analysis revealed that serotypes of Singapore, Sri Lanka, China, USA and Taiwan are likely ancestors. Serotypes of Thailand occupy an intermediate position and serotypes of Japan, Philippine and Vietnam are descendant from serotypes of Thailand.