EFFECT OF PLANT GROWTH REGULATORS IN THE PROPAGATION OF SEEDLING EXPLANT CAPSICUM ANNUUM L. VAR. ANNUUM

Abstract

This is a very initial phase of work where the object was to find out a very simple and effective process of in-vitro plant generation of Capsicum annuum L. var. annuum. For the regeneration of plants, various explants have been used including different parts of seedlings, axillary buds, and apical buds. This study revealed that basal media containing 2, 4 D (1.5mg/l.) and kinetin (0.5 mg/l.) were acting as callus-forming media. MS basal media with NAA (1.0 mg/l.) and BAP (0.5 mg/l.) was acting as callus with some roots growing media. A small quantity of kinetin along not shows any significant changes. It has been found that axillary buds and apical buds were increased in length into the kinetin (1.5mg/l.) containing media. Later axillary bud and apical buds were transferred into the MS basal media containing NAA (1.0 mg/l.) and BAP (0.5 mg/l.), which act as callus with root forming media, and help to regenerate healthy new plantlets in a short period. The survival rate of these plants is 80%. Sugar and protein estimation was done. Sugar and protein content was much higher in in-vitro plants as compared to callus.