Rhizobial diversity in shrub-tree legume-based silvopastoral systems

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Bragantia Pub Date : 2022-01-01 DOI:10.1590/1678-4499.20210336
Adriana Bezerra dos Santos, G. Fracetto, F. Fracetto, M. A. Lira Junior
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Abstract

: Silvopastoral systems based on tree legumes intercropped with forage grasses can harbor a high diversity of rhizobia, and these bacteria are good indicators of soil quality in several management systems. The objective of this work was to evaluate the morphophysiological, genetic and symbiotic diversity of cowpea [ Vigna unguiculata (L.) Walp] rhizobia from soils under silvopastoral systems based on shrub-tree legumes. The experiment was performed in a randomized block design with three treatments and three replications, consisting of signalgrass ( Urochloa decumbens Stapf.) intercropped with sabia ( Mimosa caesalpiniaefolia ); signalgrass intercropped with gliricidia ( Gliricidia sepium ) and single signalgrass. The samples were collected in the legume row (0 meter) and 4 and 8 meters away. Later, cowpea was used as a trap plant to capture the rhizobia. All strains were phenotypically characterized, authenticated, and genetically identified. Phenotypical characterization of the 431 isolates showed high diversity forming 69 groups at 100% similarity, of which 60 were able to nodulate cowpea during the authentication, and 36 presented relative efficiency superior or equal to the recommended bacteria for the crop. Most of the sequenced strains belonged to Bradyrhizobium (67%) and Methylobacterium (9%). Leifsonia (9%), Cohnella (6%), Rhizobium (3%), Burkholderia (3%), and Paenibacillus (3%) were also represented. Soils under silvopastoral systems harbor efficient rhizobia populations in cowpea with a high genetic diversity, which can be recommended for agronomic efficiency assays.
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以豆科植物为基础的灌树林系统的根瘤菌多样性
以豆科树木间作牧草为基础的森林系统可以孕育高度多样性的根瘤菌,这些细菌在一些管理系统中是土壤质量的良好指标。摘要对豇豆(Vigna unguiculata, L.)的形态、生理、遗传和共生多样性进行了研究。以灌丛豆科植物为基础的森林系统土壤中的根瘤菌。试验采用3个处理、3个重复的随机区组设计,即信号草(Urochloa decumbens Stapf.)间作含羞草(Mimosa caesalpiniaefolia);间作的信号草(gliricidia sepium)和单信号草。样品采集于豆科植物行(0米)、4米和8米处。后来,豇豆被用作捕获根瘤菌的诱捕植物。所有菌株都进行了表型表征、鉴定和遗传鉴定。431株分离菌的表型特征显示出较高的多样性,形成69个类群,类群相似性为100%,其中60个类群在鉴定过程中能够结瘤豇豆,36个类群的相对效率优于或等于作物推荐菌。大多数测序菌株属于慢生根瘤菌(67%)和甲基杆菌(9%)。Leifsonia (9%), Cohnella (6%), Rhizobium (3%), Burkholderia(3%)和Paenibacillus(3%)也有代表。在银杏系统下的土壤中,豇豆根瘤菌群具有较高的遗传多样性,可以推荐用于农艺效率分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bragantia
Bragantia AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
2.40
自引率
8.30%
发文量
33
审稿时长
4 weeks
期刊介绍: Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).
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