M. Alves, Cybelle Souza Oliveira, Gisely Moreira Vitalino, E. Carvalho, J. Oliveira, G. Fracetto, F. Fracetto, M. A. Lira Junior
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引用次数: 1
Abstract
: Some forage species, such as pangolão grass ( Digitaria eriantha Steud. cv. Survenola), are resilient in tropical semiarid regions. A possible reason for this is the presence of endophytic and rhizospheric microorganisms. Thus, this study evaluated the diversity of associative bacteria in pangolão grass. Bacteria associated with the roots, culm, leaves, and rhizospheric soil were isolated and characterized in three municipalities of Pernambuco, Brazil. An initial phenotypic characterization was followed by a genotypic assessment by based repetitive extragenic palindromic-polymerase chain reaction (BOX-PCR) and partial sequencing of the 16S rRNA gene. We obtained 325 phenotypically-characterized isolates grouped into 243 strains with 100% similarity by BOX-PCR. The most diverse sampling environment was Araripina, and all factors affected bacterial diversity. There were 135 groups with 90% similarity, that were represented by a single strain each for sequencing. Among the sequenced strains, 118 showed 96.84–99.9% similarity with previously described strains, whereas 17 could not be identified. The following 18 genera were identified from three phyla, five classes, seven orders, and 13 families: Achromobacter , Agrobacterium , Bacillus , Burkholderia , Curtobacterium , Enterobacter , Herbaspirillum , Kosakonia , Ochrobactrum , Paenibacillus , Pantoea , Priestia , Pseudomonas , Rhizobium , Serratia , Shinella , Stenotrophomonas , and Variovorax . The diversity of endophytic and rhizospheric bacteria may contribute to the resilience of pangolão , as various strains of these genera have been described as plant growth promoters. This is the first evaluation of pangolão bacterial diversity under tropical semiarid conditions. Since several of the genera include strains known to promote plant growth, we propose further research to evaluate this on crops.
期刊介绍:
Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas.
A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005.
É filiada à Associação Brasileira de Editores Científicos (ABEC).