Associative bacterial diversity of pangolão, a stressresilient tropical grass

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Bragantia Pub Date : 2022-01-01 DOI:10.1590/1678-4499.20220071
M. Alves, Cybelle Souza Oliveira, Gisely Moreira Vitalino, E. Carvalho, J. Oliveira, G. Fracetto, F. Fracetto, M. A. Lira Junior
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引用次数: 1

Abstract

: Some forage species, such as pangolão grass ( Digitaria eriantha Steud. cv. Survenola), are resilient in tropical semiarid regions. A possible reason for this is the presence of endophytic and rhizospheric microorganisms. Thus, this study evaluated the diversity of associative bacteria in pangolão grass. Bacteria associated with the roots, culm, leaves, and rhizospheric soil were isolated and characterized in three municipalities of Pernambuco, Brazil. An initial phenotypic characterization was followed by a genotypic assessment by based repetitive extragenic palindromic-polymerase chain reaction (BOX-PCR) and partial sequencing of the 16S rRNA gene. We obtained 325 phenotypically-characterized isolates grouped into 243 strains with 100% similarity by BOX-PCR. The most diverse sampling environment was Araripina, and all factors affected bacterial diversity. There were 135 groups with 90% similarity, that were represented by a single strain each for sequencing. Among the sequenced strains, 118 showed 96.84–99.9% similarity with previously described strains, whereas 17 could not be identified. The following 18 genera were identified from three phyla, five classes, seven orders, and 13 families: Achromobacter , Agrobacterium , Bacillus , Burkholderia , Curtobacterium , Enterobacter , Herbaspirillum , Kosakonia , Ochrobactrum , Paenibacillus , Pantoea , Priestia , Pseudomonas , Rhizobium , Serratia , Shinella , Stenotrophomonas , and Variovorax . The diversity of endophytic and rhizospheric bacteria may contribute to the resilience of pangolão , as various strains of these genera have been described as plant growth promoters. This is the first evaluation of pangolão bacterial diversity under tropical semiarid conditions. Since several of the genera include strains known to promote plant growth, we propose further research to evaluate this on crops.
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热带禾草穿山甲的结合力细菌多样性
:一些饲料种类,如穿山甲 (Digitaria eriantha Steud)。简历。Survenola)在热带半干旱地区具有复原力。一个可能的原因是存在内生微生物和根际微生物。因此,本研究对穿山甲草伴生菌的多样性进行了评价。与根、茎、叶和根际土壤相关的细菌在巴西伯南布哥的三个城市被分离和表征。通过重复基因外回文聚合酶链式反应(BOX-PCR)和16S rRNA基因的部分测序进行初步表型鉴定和基因型评估。我们通过BOX-PCR获得325株表型特征的分离株,分为243株,相似性为100%。采样环境以Araripina为最,各因素均影响细菌多样性。有135个相似度为90%的群体,每个群体用一个菌株来表示。测序菌株中,118株与已有菌株相似度为96.84 ~ 99.9%,17株无法鉴定。从3门5纲7目13科共鉴定出无色杆菌属、农杆菌属、芽孢杆菌属、伯氏杆菌属、Curtobacterium属、Enterobacter属、Herbaspirillum属、Kosakonia属、Ochrobactrum属、Paenibacillus属、Pantoea属、Priestia属、Pseudomonas属、Rhizobium属、Serratia属、Shinella属、窄养单胞菌属、Variovorax属。内生细菌和根际细菌的多样性可能有助于穿山甲的恢复力,因为这些属的各种菌株已被描述为植物生长促进剂。这是对热带半干旱条件下穿山甲细菌多样性的首次评估。由于一些属包括已知促进植物生长的菌株,我们建议进一步研究以评估作物的这种作用。
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来源期刊
Bragantia
Bragantia AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
2.40
自引率
8.30%
发文量
33
审稿时长
4 weeks
期刊介绍: Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).
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