Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Pesquisa Veterinaria Brasileira Pub Date : 2022-01-01 DOI:10.1590/1678-5150-pvb-7059

Andresa Guimarães, H. Santos, D. Balthazar, M. Kierulff, M. N. Baptista, Á. F. Oliveira, N. V. Stocco, Elisabeth N. Mureb, A. Costa, J. M. Raimundo, C. Baldani

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Abstract

ABSTRACT: Trypanosoma spp. infection is a problem in many tropical countries, infecting several animal species, including humans. The aim of the present study was to identify the Trypanosoma species in Neotropical primates from Rio de Janeiro state and compare the results with other reports both phylogenetically and geographically. Molecular detection was based on the 18 SSU gene. The sequences obtained in the PCR were sequenced and compared with others previously deposited in GenBank. These sequences were used to perform phylogenetic analysis and make a distribution map of primate species infected by Trypanosoma species in Brazil. Among 34 monkeys, five capuchin monkeys (Sapajus spp.) and one marmoset (Callithrix spp.) showed Trypanosoma spp. sequences in the same clade of Trypanosoma minasense and three capuchin monkeys’ sequences were in the same clade of Trypanosoma cruzi. The Atlantic Forest and the Brazilian Amazon are the regions with the highest frequency of studies about Trypanosoma spp. and variety of Neotropical primate hosts. These are areas that deserve attention regarding the conservation of biodiversity, but it also makes evident the lack of studies with Neotropical primates in other regions of the country, as well as multidisciplinary studies to better understand the host pathogen relationships.

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巴西里约热内卢州新热带灵长类动物中锥虫的分子检测和系统发育分析

摘要:锥虫感染在许多热带国家都是一个问题，它可以感染包括人类在内的多种动物。本研究的目的是鉴定巴西里约热内卢州新热带灵长类动物的锥虫种类，并将结果与其他报道的系统发育和地理位置进行比较。分子检测基于18ssu基因。对PCR获得的序列进行测序，并与先前存放在GenBank中的其他序列进行比较。这些序列被用于进行系统发育分析，并绘制了巴西被锥虫感染的灵长类物种分布图。34只猴子中，5只卷尾猴(Sapajus)和1只绒猴(Callithrix)的序列在米氏锥虫的同一支系，3只卷尾猴的序列在克氏锥虫的同一支系。大西洋森林和巴西亚马逊是研究锥虫和各种新热带灵长类宿主的频率最高的地区。在保护生物多样性方面，这些领域值得关注，但也明显缺乏对该国其他地区新热带灵长类动物的研究，以及更好地了解宿主病原体关系的多学科研究。

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来源期刊

Pesquisa Veterinaria Brasileira 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Veterinária Brasileira - Brazilian Journal of Veterinary Research (http://www.pvb.com.br), edited by the Brazilian College of Animal Pathology in partnership with the Brazilian Agricultural Research Organization (Embrapa) and in collaboration with other veterinary scientific associations, publishes original papers on animal diseases and related subjects. Critical review articles should be written in support of original investigation. The editors assume that papers submitted are not being considered for publication in other journals and do not contain material which has already been published. Submitted papers are peer reviewed. The abbreviated title of Pesquisa Veterinária Brasileira is Pesqui. Vet. Bras.