Substrate and indolebutyric acid in the rooting of pomegranate cuttings collected in two seasons

Kelly Pirola, M. Dotto, Darcieli Aparecida Cassol, Alexandre Luis Alegretti, Américo Wagner Júnior
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Abstract

The pomegranate (Punica granatum L.) presents potential for commercial exploitation as a fruit, ornamental or medicinal plant. However, for its usage in orchards, it is ideal that the seedlings come from vegetative propagation techniques. The aim of this work was to test the usage of indolebutyric acid (IBA) and substrates, at two harvesting times (before and after fruiting) in the cutting propagation of pomegranate. The work was carried out at UTFPR, Campus Dois Vizinhos. The experiments were installed in a randomized block design, in a 4 x 4 factorial scheme (IBA concentration x substrate), with four replications and ten cuttings per experimental unit. IAB was tested at concentrations 0; 1000; 2000 and 3000 mg L-1. The used substrates were Red Latosol, medium textured sand, commercial substrate MecPlant® and a mixture of Red Latosol + sand + commercial (1:1:1, v/v). The cuttings were placed in beds in a screened protected environment, with 50% shading. Irrigation was performed three times a day for 30 min., by a micro sprinkler. After 60 days, rooting (%), cuttings with callus (%), number of primary leaf and root shoots, length of the three largest roots and percentage of dead cuttings were evaluated. Cutting presented itself as a potential technique to be adopted for pomegranate, using sand, with the use of IBA at concentrations between 1600 and 1750 mg L-1. Cuttings must be collected before fruiting. However, rhizogenesis close to 50% was achieved at the time after such phenological stage.
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两季采收的石榴插枝生根的基质和吲哚丁酸
石榴(Punica granatum L.)作为一种水果、观赏或药用植物具有潜在的商业开发潜力。然而,为了在果园中使用,最理想的是幼苗来自无性繁殖技术。本研究的目的是测试吲哚丁酸(IBA)和底物在两个采收期(结果前后)在石榴扦插繁殖中的使用情况。这项工作是在Dois Vizinhos校区的UTFPR进行的。实验采用随机区组设计,采用4 × 4因子方案(IBA浓度x底物),4次重复,每个实验单元10个插片。IAB检测浓度为0;1000;2000和3000毫克L-1。所使用的基质为红色Latosol、中等纹理砂、商用基质MecPlant®和红色Latosol +砂+商用(1:1:1,v/v)的混合物。插枝被放置在有遮挡的保护环境中的床上,有50%的遮阳。每天进行三次灌溉,每次30分钟,用微型喷灌器。60 d后测定生根率(%)、带愈伤组织插条数(%)、初生叶数和根芽数、最大3根长度和死插条率。切割是石榴的一种潜在技术,使用沙子,使用浓度在1600和1750 mg L-1之间的IBA。插枝必须在结果前收集。然而,在这一物候阶段之后,根的发生接近50%。
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