Extraction, Toxicity Assay and Identification of Active Compounds of Red Algae (Eucheuma cottonii) from Sumenep Madura

Abstract

Extraction, Toxicity Assay and Identification ofActive Compounds of Red Algae Extract (Eucheuma cottonii ) from Tanjung Sumenep Madura was analysed. Extraction of active compound was conducted by maceration method  using methanol as a solvent. The methanol extract was hydrolyzed with HCl 2 N. The result was partitioned using five solvents (1-butanol, ethyl acetate, chloroform, petroleum  ether and n-hexane). Toxicity level was determined by Brine Shrimp Lethality Test (BSLT). The identification of the active compound was analyzed by phytochemical test, qualitatively. The result showed that the 1-butanol is the best solvent to extract active compounds of red algae E. Cottonii in partition process. 1-Butanol fraction gives the highest activity of toxicity with LC 50 70,32 ppm. LC 50 value for ethyl acetate, chloroform, petroleum ether and n-hexane was 143,43; 194,40; 195,28; 303,66; and 634,97 ppm, respectively. Identification of active compounds indicated that 1-butanol fraction contains steroids and alkaloids. Keywords : Eucheuma cottonii , extraction, Brine Shrimp Lethality Test, phythochemical