E-test antibiotic susceptibility of E.coli strains isolated from hospital acquired infections of Imam Khomeini hospital, Ilam, Iran

Abstract

Introduction: Escherichia coli (E.coli) as a main cause of both nosocomial and communityacquired infections in humans have a relative potential to develop resistance. Nowadays, most infections caused by ESBL-producing E.coli (ESBLEC) had mostly been described as nosocomial acquired or nursing home related. In this study, we employed E-test assay to detect antibiotic resistance of E.coli strains and determine MIC of antibiotics. Materials and methods: Thirty E.coli strains gathered from Imam Khomeini hospital of Ilam, and cultured on TSB and bacterial suspension prepared by 0.5 μF concentration for Etest. Mueller Hinton agar and E-test strips of Amikacin, Cefepime, Ceftazidime, Ceftriaxone, Gentamicin, Meropenem, Nitrofurantoin, Piperacillin/Tazobactam, Tetracycline, Ticarcillin/ Clavulanic acid, Tobramycin, Trimethoprim were used Results: Resistance to Ceftriaxone, Tobramycin, Gentamicin, Ticarcillin/ Clavulanic, Amikacin were 19.8%, 26.4%, 23.3%, 62.7%, 70.3%, respectively. Conclusion: the results indicated, E.coli strains in this study were high sensitivity to Meropenem ,Nitrofuratoin, Ciprofloxacin , Ceftazidime, Cefepime.