Zijun Zhao, Yutao Zhang*, Menglan Wu, Chenxu Yan and Zhiqian Guo*,
{"title":"A Reversible Dual-Channel Near-Infrared Flavonoid Probe for in Vivo Tracking Glutathione Dynamics in Living Mice","authors":"Zijun Zhao, Yutao Zhang*, Menglan Wu, Chenxu Yan and Zhiqian Guo*, ","doi":"10.1021/cbmi.3c00051","DOIUrl":null,"url":null,"abstract":"<p >Glutathione (GSH) plays a critical role in various biological processes maintaining oxidative homeostasis. However, current reversible probe fluorescence emission is usually in the visible region, making it difficult to monitor glutathione levels in deep tissues and <i>in vivo</i>. Here, we developed a reversible near-infrared fluorescence probe, Flav-N, for real-time tracking of GSH in cells and tissues, which undergoes fast and reversible Michael addition reactions with biothiols. This Flav-N probe showed a rapid and reversible response with GSH at a time of less than 5 s (<i>k</i> = 1286 M<sup>–1</sup>S<sup>–1</sup>, <i>t</i><sub>1/2</sub> = 729 ms). Notably, the dynamic changes in the ratio of Flav-N emission intensity at 505 and 728 nm were able to provide real-time feedback on the fluctuation of GSH concentration. We demonstrated that Flav-N enables the performance of fast and reversible imaging of intracellular GSH changes. Importantly, in light of the near-infrared emission and rapid response ability, Flav-N was successfully applied to track GSH dynamics in living mice. This reversible near-infrared NIR probe realizes advances in deep insight into the function of endogenous GSH.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00051","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical & Biomedical Imaging","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/cbmi.3c00051","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Glutathione (GSH) plays a critical role in various biological processes maintaining oxidative homeostasis. However, current reversible probe fluorescence emission is usually in the visible region, making it difficult to monitor glutathione levels in deep tissues and in vivo. Here, we developed a reversible near-infrared fluorescence probe, Flav-N, for real-time tracking of GSH in cells and tissues, which undergoes fast and reversible Michael addition reactions with biothiols. This Flav-N probe showed a rapid and reversible response with GSH at a time of less than 5 s (k = 1286 M–1S–1, t1/2 = 729 ms). Notably, the dynamic changes in the ratio of Flav-N emission intensity at 505 and 728 nm were able to provide real-time feedback on the fluctuation of GSH concentration. We demonstrated that Flav-N enables the performance of fast and reversible imaging of intracellular GSH changes. Importantly, in light of the near-infrared emission and rapid response ability, Flav-N was successfully applied to track GSH dynamics in living mice. This reversible near-infrared NIR probe realizes advances in deep insight into the function of endogenous GSH.
期刊介绍:
Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging