Pub Date : 2024-08-22DOI: 10.1021/cbmi.4c0003010.1021/cbmi.4c00030
Majed Shabsigh, and , Lee A. Solomon*,
The development of peptide-based, radiometal-labeled PET imaging agents has seen an increase in attention due to the favorable properties the peptide backbone exhibits. These include high selectivity and affinity to proteins and cells directly linked to various types of cancers. In addition, rapid clearance from circulation and low toxicity allow for unique approaches to engineering a viable peptide-based imaging agent. Utilizing peptides as the backbone allows for various modifications to improve metabolic stability, target cell affinity, and image quality and imaging capabilities and reduce toxicity. Select radiolabeled peptides have already been FDA approved, with many more in late-stage trials. This review summarizes the current state of the radiometal-labeled PET peptide imaging field as well as explores methods used by researchers to modify peptides, concluding with a look at the future of peptide-based therapy and diagnostics.
由于多肽骨架所具有的良好特性,以多肽为基础的放射性金属标记 PET 成像剂的开发越来越受到关注。这些特性包括对与各类癌症直接相关的蛋白质和细胞具有高选择性和亲和性。此外,肽在血液循环中的快速清除和低毒性使我们可以采用独特的方法来设计一种可行的肽基成像剂。利用肽作为骨架可以进行各种修饰,以提高代谢稳定性、靶细胞亲和力、成像质量和成像能力,并降低毒性。部分放射性标记肽已获得 FDA 批准,还有更多肽处于后期试验阶段。本综述总结了放射性同位素标记 PET 肽成像领域的现状,并探讨了研究人员用来修饰肽的方法,最后展望了基于肽的治疗和诊断的未来。
{"title":"Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine","authors":"Majed Shabsigh, and , Lee A. Solomon*, ","doi":"10.1021/cbmi.4c0003010.1021/cbmi.4c00030","DOIUrl":"https://doi.org/10.1021/cbmi.4c00030https://doi.org/10.1021/cbmi.4c00030","url":null,"abstract":"<p >The development of peptide-based, radiometal-labeled PET imaging agents has seen an increase in attention due to the favorable properties the peptide backbone exhibits. These include high selectivity and affinity to proteins and cells directly linked to various types of cancers. In addition, rapid clearance from circulation and low toxicity allow for unique approaches to engineering a viable peptide-based imaging agent. Utilizing peptides as the backbone allows for various modifications to improve metabolic stability, target cell affinity, and image quality and imaging capabilities and reduce toxicity. Select radiolabeled peptides have already been FDA approved, with many more in late-stage trials. This review summarizes the current state of the radiometal-labeled PET peptide imaging field as well as explores methods used by researchers to modify peptides, concluding with a look at the future of peptide-based therapy and diagnostics.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31eCollection Date: 2024-09-23DOI: 10.1021/cbmi.4c00031
Reza Reihanisaransari, Chalapathi Charan Gajjela, Xinyu Wu, Ragib Ishrak, Yanping Zhong, David Mayerich, Sebastian Berisha, Rohith Reddy
Hyperspectral photothermal mid-infrared spectroscopic imaging (HP-MIRSI) is an emerging technology with promising applications in cervical cancer diagnosis and quantitative, label-free histopathology. This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues, achieving excellent tissue type segmentation accuracy of over 95%. This achievement stems from an integrated approach of optimized data acquisition, computational data reconstruction, and the application of machine learning algorithms. The results are statistically robust, drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points. Traditional cervical cancer diagnosis methods entail biopsy, staining, and visual evaluation by a pathologist. This process is qualitative, subject to variations in staining and subjective interpretations, and requires extensive tissue processing, making it costly and time-consuming. In contrast, our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation. This label-free, quantitative method utilizes biochemical data from HP-MIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes. This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.
{"title":"Cervical Cancer Tissue Analysis Using Photothermal Midinfrared Spectroscopic Imaging.","authors":"Reza Reihanisaransari, Chalapathi Charan Gajjela, Xinyu Wu, Ragib Ishrak, Yanping Zhong, David Mayerich, Sebastian Berisha, Rohith Reddy","doi":"10.1021/cbmi.4c00031","DOIUrl":"https://doi.org/10.1021/cbmi.4c00031","url":null,"abstract":"<p><p>Hyperspectral photothermal mid-infrared spectroscopic imaging (HP-MIRSI) is an emerging technology with promising applications in cervical cancer diagnosis and quantitative, label-free histopathology. This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues, achieving excellent tissue type segmentation accuracy of over 95%. This achievement stems from an integrated approach of optimized data acquisition, computational data reconstruction, and the application of machine learning algorithms. The results are statistically robust, drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points. Traditional cervical cancer diagnosis methods entail biopsy, staining, and visual evaluation by a pathologist. This process is qualitative, subject to variations in staining and subjective interpretations, and requires extensive tissue processing, making it costly and time-consuming. In contrast, our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation. This label-free, quantitative method utilizes biochemical data from HP-MIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes. This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1021/cbmi.4c0003110.1021/cbmi.4c00031
Reza Reihanisaransari, Chalapathi Charan Gajjela, Xinyu Wu, Ragib Ishrak, Yanping Zhong, David Mayerich, Sebastian Berisha and Rohith Reddy*,
Hyperspectral photothermal mid-infrared spectroscopic imaging (HP-MIRSI) is an emerging technology with promising applications in cervical cancer diagnosis and quantitative, label-free histopathology. This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues, achieving excellent tissue type segmentation accuracy of over 95%. This achievement stems from an integrated approach of optimized data acquisition, computational data reconstruction, and the application of machine learning algorithms. The results are statistically robust, drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points. Traditional cervical cancer diagnosis methods entail biopsy, staining, and visual evaluation by a pathologist. This process is qualitative, subject to variations in staining and subjective interpretations, and requires extensive tissue processing, making it costly and time-consuming. In contrast, our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation. This label-free, quantitative method utilizes biochemical data from HP-MIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes. This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.
{"title":"Cervical Cancer Tissue Analysis Using Photothermal Midinfrared Spectroscopic Imaging","authors":"Reza Reihanisaransari, Chalapathi Charan Gajjela, Xinyu Wu, Ragib Ishrak, Yanping Zhong, David Mayerich, Sebastian Berisha and Rohith Reddy*, ","doi":"10.1021/cbmi.4c0003110.1021/cbmi.4c00031","DOIUrl":"https://doi.org/10.1021/cbmi.4c00031https://doi.org/10.1021/cbmi.4c00031","url":null,"abstract":"<p >Hyperspectral photothermal mid-infrared spectroscopic imaging (HP-MIRSI) is an emerging technology with promising applications in cervical cancer diagnosis and quantitative, label-free histopathology. This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues, achieving excellent tissue type segmentation accuracy of over 95%. This achievement stems from an integrated approach of optimized data acquisition, computational data reconstruction, and the application of machine learning algorithms. The results are statistically robust, drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points. Traditional cervical cancer diagnosis methods entail biopsy, staining, and visual evaluation by a pathologist. This process is qualitative, subject to variations in staining and subjective interpretations, and requires extensive tissue processing, making it costly and time-consuming. In contrast, our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation. This label-free, quantitative method utilizes biochemical data from HP-MIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes. This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30eCollection Date: 2024-09-23DOI: 10.1021/cbmi.4c00032
Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N Kramer, Surajit Chatterjee, Lydia Kisley
Correlation signal processing of optical three-dimensional (x, y, t) data can produce super-resolution images. The second-order cross-correlation function XC2 has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and numerically demonstrate cross-correlation analysis for diffusing particles. We then expand our fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) analysis to use cross-correlation as a postprocessing computational technique to extract both dynamic and structural information on particle diffusion in nanoscale structures simultaneously. Cross-correlation maintains the same super-resolution as auto-correlation while also increasing the sampling rates to reduce aliasing for spatial information in both simulated and experimental data. Our work demonstrates how fcsSOFI with cross-correlation can be a powerful signal-processing tool to resolve the nanoscale dynamics and structure in samples relevant to biological and soft materials.
{"title":"Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging.","authors":"Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N Kramer, Surajit Chatterjee, Lydia Kisley","doi":"10.1021/cbmi.4c00032","DOIUrl":"https://doi.org/10.1021/cbmi.4c00032","url":null,"abstract":"<p><p>Correlation signal processing of optical three-dimensional (<i>x</i>, <i>y</i>, <i>t</i>) data can produce super-resolution images. The second-order cross-correlation function <i>XC</i> <sub>2</sub> has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and numerically demonstrate cross-correlation analysis for diffusing particles. We then expand our fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) analysis to use cross-correlation as a postprocessing computational technique to extract both dynamic and structural information on particle diffusion in nanoscale structures simultaneously. Cross-correlation maintains the same super-resolution as auto-correlation while also increasing the sampling rates to reduce aliasing for spatial information in both simulated and experimental data. Our work demonstrates how fcsSOFI with cross-correlation can be a powerful signal-processing tool to resolve the nanoscale dynamics and structure in samples relevant to biological and soft materials.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.1021/cbmi.4c0003210.1021/cbmi.4c00032
Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N. Kramer, Surajit Chatterjee and Lydia Kisley*,
Correlation signal processing of optical three-dimensional (x, y, t) data can produce super-resolution images. The second-order cross-correlation function XC2 has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and numerically demonstrate cross-correlation analysis for diffusing particles. We then expand our fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) analysis to use cross-correlation as a postprocessing computational technique to extract both dynamic and structural information on particle diffusion in nanoscale structures simultaneously. Cross-correlation maintains the same super-resolution as auto-correlation while also increasing the sampling rates to reduce aliasing for spatial information in both simulated and experimental data. Our work demonstrates how fcsSOFI with cross-correlation can be a powerful signal-processing tool to resolve the nanoscale dynamics and structure in samples relevant to biological and soft materials.
{"title":"Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging","authors":"Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N. Kramer, Surajit Chatterjee and Lydia Kisley*, ","doi":"10.1021/cbmi.4c0003210.1021/cbmi.4c00032","DOIUrl":"https://doi.org/10.1021/cbmi.4c00032https://doi.org/10.1021/cbmi.4c00032","url":null,"abstract":"<p >Correlation signal processing of optical three-dimensional (<i>x</i>, <i>y</i>, <i>t</i>) data can produce super-resolution images. The second-order cross-correlation function <i>XC</i><sub>2</sub> has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and numerically demonstrate cross-correlation analysis for diffusing particles. We then expand our fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) analysis to use cross-correlation as a postprocessing computational technique to extract both dynamic and structural information on particle diffusion in nanoscale structures simultaneously. Cross-correlation maintains the same super-resolution as auto-correlation while also increasing the sampling rates to reduce aliasing for spatial information in both simulated and experimental data. Our work demonstrates how fcsSOFI with cross-correlation can be a powerful signal-processing tool to resolve the nanoscale dynamics and structure in samples relevant to biological and soft materials.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1021/cbmi.4c0004510.1021/cbmi.4c00045
David Ken Gibbs*, Maximilian Podsednik, Patrick Tapler, Maximilian Weiss, Alexander Karl Opitz, Michael Nelhiebel, Charles Derrick Quarles Jr, Silvia Larisegger and Andreas Limbeck*,
Elemental imaging in laser-induced breakdown spectroscopy is usually performed by placing laser shots adjacent to each other on the sample surface without spatial overlap. Seeing that signal intensity is directly related to the amount of ablated material, this restricts either spatial resolution (for a given excitation efficiency) or sensitivity (when reducing the laser spot size). The experimental applicability of a concept involving the spatial overlapping of shots on the sample surface is investigated and compared to the conventional approach. By systematic choice of spacing between laser shots, spatial resolution can be improved to the single digit micrometer range for a given laser spot size. Signal intensity is found to be linearly dependent on the area ablated per shot, facilitating larger signal-to-background ratios with increased spot sizes. Owing to this, the presented approach is also employed to enhance signal intensity, while preserving spatial resolution. The applicability of the method is explored by analyzing samples with distinct thickness of the surface layer, allowing for the assessment of the concept’s suitability for different sample types.
{"title":"Improving Spatial Resolution by Reinterpreting Dosage for Laser-Induced Breakdown Spectroscopy Imaging: Conceptualization and Limitations","authors":"David Ken Gibbs*, Maximilian Podsednik, Patrick Tapler, Maximilian Weiss, Alexander Karl Opitz, Michael Nelhiebel, Charles Derrick Quarles Jr, Silvia Larisegger and Andreas Limbeck*, ","doi":"10.1021/cbmi.4c0004510.1021/cbmi.4c00045","DOIUrl":"https://doi.org/10.1021/cbmi.4c00045https://doi.org/10.1021/cbmi.4c00045","url":null,"abstract":"<p >Elemental imaging in laser-induced breakdown spectroscopy is usually performed by placing laser shots adjacent to each other on the sample surface without spatial overlap. Seeing that signal intensity is directly related to the amount of ablated material, this restricts either spatial resolution (for a given excitation efficiency) or sensitivity (when reducing the laser spot size). The experimental applicability of a concept involving the spatial overlapping of shots on the sample surface is investigated and compared to the conventional approach. By systematic choice of spacing between laser shots, spatial resolution can be improved to the single digit micrometer range for a given laser spot size. Signal intensity is found to be linearly dependent on the area ablated per shot, facilitating larger signal-to-background ratios with increased spot sizes. Owing to this, the presented approach is also employed to enhance signal intensity, while preserving spatial resolution. The applicability of the method is explored by analyzing samples with distinct thickness of the surface layer, allowing for the assessment of the concept’s suitability for different sample types.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-08eCollection Date: 2024-08-26DOI: 10.1021/cbmi.4c00020
David R Smith, Jesse W Wilson, Siddarth Shivkumar, Hervé Rigneault, Randy A Bartels
We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump-probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.
{"title":"Low-Frequency Coherent Raman Imaging Robust to Optical Scattering.","authors":"David R Smith, Jesse W Wilson, Siddarth Shivkumar, Hervé Rigneault, Randy A Bartels","doi":"10.1021/cbmi.4c00020","DOIUrl":"10.1021/cbmi.4c00020","url":null,"abstract":"<p><p>We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump-probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11351428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142117047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-08DOI: 10.1021/cbmi.4c0002010.1021/cbmi.4c00020
David R. Smith, Jesse W. Wilson, Siddarth Shivkumar, Hervé Rigneault and Randy A. Bartels*,
We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump–probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.
{"title":"Low-Frequency Coherent Raman Imaging Robust to Optical Scattering","authors":"David R. Smith, Jesse W. Wilson, Siddarth Shivkumar, Hervé Rigneault and Randy A. Bartels*, ","doi":"10.1021/cbmi.4c0002010.1021/cbmi.4c00020","DOIUrl":"https://doi.org/10.1021/cbmi.4c00020https://doi.org/10.1021/cbmi.4c00020","url":null,"abstract":"<p >We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump–probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142075613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-02DOI: 10.1021/cbmi.4c0003910.1021/cbmi.4c00039
Apeksha C. Rajamanthrilage, Unaiza Uzair, Paul W. Millhouse, Matthew J. Case, Donald W. Benza and Jeffrey N. Anker*,
Measuring chemical concentrations at the surface of implanted medical devices is important for elucidating the local biochemical environment, especially during implant infection. Although chemical indicator dyes enable chemical measurements in vitro, they are usually ineffective when measuring through tissue because the background obscures the dye signal and scattering dramatically reduces the spatial resolution. X-ray excited luminescent chemical imaging (XELCI) is a recent imaging modality which overcomes these limitations using a focused X-ray beam to excite a small spot of red light on scintillator-coated medical implants with well-defined location (because X-rays are minimally scattered) and low background. A spectrochemical indicator film placed over the scintillator layer, e.g., a polymer film containing pH-indicator dyes, absorbs some of the luminescence according to the local chemical environment, and this absorption is then detected by measuring the light intensity/spectrum passing through the tissue. A focused X-ray beam is used to scan point-by-point with a spatial resolution mainly limited by the X-ray beam width with minimum increase from X-ray absorption and scattering in the tissue. X-ray resolution, implant surface specificity, and chemical sensitivity are the three key features of XELCI. Here, we study spatial resolution using optically absorptive targets. For imaging a series of lines, the 20–80% knife-edge resolution was ∼285 (±15) μm with no tissue and 475 ± 18 and 520 ± 34 μm, respectively, through 5 and 10 mm thick tissue. Thus, doubling the tissue depth did not appreciably change the spatial resolution recorded through the tissue. This shows the promise of XELCI for submillimeter chemical imaging through tissue.
测量植入式医疗器械表面的化学浓度对于阐明局部生化环境非常重要,尤其是在植入物感染期间。虽然化学指示剂染料可以在体外进行化学测量,但在通过组织进行测量时通常效果不佳,因为背景会掩盖染料信号,而且散射会大大降低空间分辨率。X 射线激发发光化学成像(XELCI)是最近出现的一种成像方式,它克服了这些局限性,利用聚焦 X 射线束在闪烁体涂层的医疗植入物上激发一小点红光,具有位置明确(因为 X 射线散射最小)和背景低的特点。放置在闪烁体层上的光谱化学指示膜(如含有 pH 值指示染料的聚合物膜)会根据当地的化学环境吸收部分发光,然后通过测量穿过组织的光强/光谱来检测这种吸收。聚焦 X 射线束用于逐点扫描,其空间分辨率主要受 X 射线束宽度的限制,组织中 X 射线吸收和散射的影响最小。X 射线分辨率、植入物表面特异性和化学灵敏度是 XELCI 的三大特点。在此,我们利用光学吸收目标研究空间分辨率。在对一系列线条成像时,无组织时 20-80% 的刀口分辨率为 ∼285 (±15) μm,而通过 5 毫米和 10 毫米厚的组织时,分辨率分别为 475 ± 18 μm 和 520 ± 34 μm。因此,将组织深度增加一倍并不会明显改变通过组织记录的空间分辨率。这表明 XELCI 有希望通过组织进行亚毫米化学成像。
{"title":"Spatial Resolution for X-ray Excited Luminescence Chemical Imaging (XELCI)","authors":"Apeksha C. Rajamanthrilage, Unaiza Uzair, Paul W. Millhouse, Matthew J. Case, Donald W. Benza and Jeffrey N. Anker*, ","doi":"10.1021/cbmi.4c0003910.1021/cbmi.4c00039","DOIUrl":"https://doi.org/10.1021/cbmi.4c00039https://doi.org/10.1021/cbmi.4c00039","url":null,"abstract":"<p >Measuring chemical concentrations at the surface of implanted medical devices is important for elucidating the local biochemical environment, especially during implant infection. Although chemical indicator dyes enable chemical measurements in vitro, they are usually ineffective when measuring through tissue because the background obscures the dye signal and scattering dramatically reduces the spatial resolution. X-ray excited luminescent chemical imaging (XELCI) is a recent imaging modality which overcomes these limitations using a focused X-ray beam to excite a small spot of red light on scintillator-coated medical implants with well-defined location (because X-rays are minimally scattered) and low background. A spectrochemical indicator film placed over the scintillator layer, e.g., a polymer film containing pH-indicator dyes, absorbs some of the luminescence according to the local chemical environment, and this absorption is then detected by measuring the light intensity/spectrum passing through the tissue. A focused X-ray beam is used to scan point-by-point with a spatial resolution mainly limited by the X-ray beam width with minimum increase from X-ray absorption and scattering in the tissue. X-ray resolution, implant surface specificity, and chemical sensitivity are the three key features of XELCI. Here, we study spatial resolution using optically absorptive targets. For imaging a series of lines, the 20–80% knife-edge resolution was ∼285 (±15) μm with no tissue and 475 ± 18 and 520 ± 34 μm, respectively, through 5 and 10 mm thick tissue. Thus, doubling the tissue depth did not appreciably change the spatial resolution recorded through the tissue. This shows the promise of XELCI for submillimeter chemical imaging through tissue.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141955872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}