Evaluation of the Anti-tuberculosis and Cytotoxic Potential of the Seaweed Padina australis

Abstract

Background: Marine seaweeds synthesize different types of compounds with various biological activities, including anti-tuberculosis and anticancer effects. Objective: The aim of this study was to evaluate anti tuberculosis of Padina australis and cytotoxicity of different fractions of this seaweed. Methods: Padina australis was collected from coasts of Persian Gulf, identified and extracted by maceration with methanol-ethyl acetate. The extract was evaporated and partitioned by Kupchan method to yield Hexane, dichloromethane, Butanol and water partitions. The anti-tuberculosis activity of the crude extract and cytotoxicity of fractions were investigated using GFPMA and MTT methods. Results: The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. The IC50 value of hexane, dichloromethane, butanol and water partitions were 2.0, 20. , 19.7 and 182.7 μg/ml respectively. The crude extract was not active against tuberculosis. Conclusion: This study reveals that different partitions of Padina australis have cytotoxic activity against cancer cell lines.