Anthelmintic resistance status of gastrointestinal nematodes of sheep to the single or combined administration of benzimidazoles and closantel in three localities in Mexico

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2016-12-07 DOI:10.21753/VMOA.3.4.374
Yazmín Alcalá Canto, L. Camberos, H. S. López, Lilia Gutiérrez Olvera, G. Pérez
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引用次数: 3

Abstract

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Alcala Canto Y, Sumano Lopez HS, Ocampo Camberos L, Gutierrez L. Anthelmintic resistance status of gastrointestinal nematodes of sheep to the single or combined administration of benzimidazoles and closantel in three localities in Mexico. Veterinaria Mexico OA. 2016;3(4). doi: 10.21753/vmoa.3.4.374 Sheep production requires the constant assessment of parasitic burden and the efficacy of existing treatments for proper management. In this study, the administration of five different treatments was evaluated for the reduction of the percentage of eggs per gram of feces (EPG) shed by gastrointestinal nematodes (GIN) from sheep on three different sheep-breeding farms in Mexico (Texcoco, Estado de Mexico; Hueytamalco, Puebla; and Tlaltizapan de Zapata, Morelos). In these farms, ivermectin and benzimidazole derivatives had been routinely administered for two consecutive years. To determine whether drugs with different pharmacological properties decreased GIN fecal egg excretion, the treatments closantel (CLOS), albendazole (ABZ) and fenbendazole (FBZ) were administered alone and in combinations of CLOS + ABZ and CLOS + FBZ, to five groups of sheep, with an additional untreated control group on each farm (n = 28 per flock). Anthelmintic resistance was determined using Fecal Egg Count Reduction Tests (FECRT) as recommended in the guidelines of the World Association for the Advancement of Veterinary Parasitology. Fecal samples were collected 14 and 21 days after treatment. The anthelmintic resistance status was determined based on the reduction in the fecal egg count arithmetic mean and 95 % confidence limits. According to the FECRT, resistance developed to CLOS, ABZ, FBZ and CLOS + FBZ because the mean percentage of EPG reduction was ≤ 95 % with a lower confidence limit of ≤ 90 %. By contrast, nematode susceptibility was confirmed for the CLOS + ABZ combination, as it reduced the percentage of GIN fecal egg output by 96.46 ± 3.04 % (day 14) and 96.88 ± 3.04 % (day 21). Based on the morphometric identification of larvae, Haemonchus spp., Cooperia spp. and Teladorsagia spp. were the most abundant genera on all farms before the administration of these five treatments. In conclusion, the use of the anthelmintic combination of closantel plus albendazole may reduce the development of anthelmintic resistance in gastrointestinal nematodes. Figure 1. Marginal means ± SD of the percentage reduction in egg count on days 14 and 21 after treatment of sheep on three farms (n = 28 per flock) with fenbendazole (FBZ), closantel (CLOS), albendazole (ABZ), closantel + fenbendazole (CLOS + FBZ) and closantel + albendazole (CLOS + ABZ).
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墨西哥3个地区绵羊胃肠道线虫对苯并咪唑和closantel单药或联合用药的抗药性状况
引用本文:Alcala Canto Y, Sumano Lopez HS, Ocampo Camberos L, Gutierrez L.墨西哥三个地区绵羊胃肠道线虫对苯并咪唑和closantel单药或联合用药的抗性状况。墨西哥兽医局。2016; 3(4)。绵羊生产需要不断评估寄生负担和现有治疗方法的有效性,以便进行适当管理。在本研究中,对墨西哥三个不同的绵羊养殖场(Texcoco, Estado de Mexico;Hueytamalco普埃布拉;以及莫雷洛斯州的Tlaltizapan de Zapata)。在这些农场,伊维菌素和苯并咪唑衍生物已连续两年常规使用。为了确定不同药理学性质的药物是否会减少GIN粪蛋的排出,对5组羊单独给予closantel (CLOS)、阿苯达唑(ABZ)和芬苯达唑(FBZ)治疗,以及CLOS + ABZ和CLOS + FBZ联合用药,每个农场另设未经治疗的对照组(n = 28 /羊群)。根据世界兽医寄生虫学促进协会指南的建议,使用粪卵计数减少试验(FECRT)确定了驱虫耐药性。治疗后14天和21天分别采集粪便样本。根据粪卵数算术平均值和95%置信限的减少来确定抗虫状况。根据FECRT,对CLOS、ABZ、FBZ和CLOS + FBZ产生耐药,因为EPG还原的平均百分比≤95%,下限置信限≤90%。相比之下,CLOS + ABZ组合对线虫敏感,使GIN粪卵排出率降低96.46±3.04%(第14天)和96.88±3.04%(第21天)。幼虫形态鉴定结果显示,在这5种处理前,所有养殖场的幼虫数量最多的属为Haemonchus spp、Cooperia spp和Teladorsagia spp。综上所述,使用closantel +阿苯达唑联合驱虫药可减少胃肠道线虫驱虫药耐药性的发生。图1所示。3个羊场(n = 28只/只)分别用芬苯达唑(FBZ)、closantel (CLOS)、阿苯达唑(ABZ)、closantel + fen苯达唑(CLOS + FBZ)和closantel +阿苯达唑(CLOS + ABZ)处理后第14和21天的蛋数减少百分率的边际平均值±SD。
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
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>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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