Effects of sodium chloride and methylthioninium chloride on Persian sturgeon, Acipenser persicus (Borodin, 1897): A histopathological and bacteriological study

Q3 Agricultural and Biological Sciences caspian journal of environmental sciences Pub Date : 2021-01-01 DOI:10.22124/CJES.2021.4308
R. Fathollahi, H. Khara, Z. Pajand, A. Shenavar
{"title":"Effects of sodium chloride and methylthioninium chloride on Persian sturgeon, Acipenser persicus (Borodin, 1897): A histopathological and bacteriological study","authors":"R. Fathollahi, H. Khara, Z. Pajand, A. Shenavar","doi":"10.22124/CJES.2021.4308","DOIUrl":null,"url":null,"abstract":"360 pieces of Persian sturgeon fingerlings (1.94 ± 0.75 g) were exposed to various sublethal doses of disinfecting chemicals commonly used in aquaculture such as sodium chloride and methylthioninium chloride in order to investigate their impacts on bacterial loads of skin, gill and surrounding water and to define the histopathological status of gill and liver tissues. The sublethal concentrations were determined after a pre-test, then the experiment was performed  in four treatments with three replicates inside the glass aquariums with stocking density of 10 fish (1-3 g) per aquarium. The treatments included 5, 6.3, 8.06 and 10.23 mg L-1 sodium chloride and  also 1, 1.56, 2.45 and 3.83 mg L-1 methylthioninium chloride. A control group (with no chemical added) was set up for each experiment. After exposure to treatments during 96 h, the microbial and histopathological examinations were carried out. Hemorrhage, elongation of secondary lamellae, adhesion of secondary lamellae, hypertrophy of supporter cartilage, mucus coagulation and secretion, hyperplasia, lamellar necrosis and clubbing of gill lamellae were observed on the fish gills. Biliary depression, hemorrhage, cell necrosis and degeneration of lipid were also found in liver. The severity of these effects was  dose-dependent. Only the sublethal concentration of methylthioninium chloride significantly influenced bacterial load (CFU g-1) on skin, while other treatments of sodium chloride and methylthioninium chloride did not exhibit any significant effects. In conclusion, the sublethal doses of methylthioninium chloride and sodium chloride showed no obvious disinfecting effect on gill, skin and surrounding water of Persian sturgeon fingerling. Nevertheless, histopathological alterations were observed on fish gill, skin and also  liver of all treatments.","PeriodicalId":9640,"journal":{"name":"caspian journal of environmental sciences","volume":"19 1","pages":"75-83"},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"caspian journal of environmental sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22124/CJES.2021.4308","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1

Abstract

360 pieces of Persian sturgeon fingerlings (1.94 ± 0.75 g) were exposed to various sublethal doses of disinfecting chemicals commonly used in aquaculture such as sodium chloride and methylthioninium chloride in order to investigate their impacts on bacterial loads of skin, gill and surrounding water and to define the histopathological status of gill and liver tissues. The sublethal concentrations were determined after a pre-test, then the experiment was performed  in four treatments with three replicates inside the glass aquariums with stocking density of 10 fish (1-3 g) per aquarium. The treatments included 5, 6.3, 8.06 and 10.23 mg L-1 sodium chloride and  also 1, 1.56, 2.45 and 3.83 mg L-1 methylthioninium chloride. A control group (with no chemical added) was set up for each experiment. After exposure to treatments during 96 h, the microbial and histopathological examinations were carried out. Hemorrhage, elongation of secondary lamellae, adhesion of secondary lamellae, hypertrophy of supporter cartilage, mucus coagulation and secretion, hyperplasia, lamellar necrosis and clubbing of gill lamellae were observed on the fish gills. Biliary depression, hemorrhage, cell necrosis and degeneration of lipid were also found in liver. The severity of these effects was  dose-dependent. Only the sublethal concentration of methylthioninium chloride significantly influenced bacterial load (CFU g-1) on skin, while other treatments of sodium chloride and methylthioninium chloride did not exhibit any significant effects. In conclusion, the sublethal doses of methylthioninium chloride and sodium chloride showed no obvious disinfecting effect on gill, skin and surrounding water of Persian sturgeon fingerling. Nevertheless, histopathological alterations were observed on fish gill, skin and also  liver of all treatments.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
氯化钠和甲基硫鎓氯对波斯鲟鱼的影响,acpenser persicus (Borodin, 1897):组织病理学和细菌学研究
将360条波斯鲟鱼种(1.94±0.75 g)暴露于各种亚致死剂量的水产养殖常用消毒化学品,如氯化钠和甲基硫代氯化铵,以研究它们对皮肤、鳃和周围水细菌负荷的影响,并确定鳃和肝脏组织的组织病理学状态。预试后测定亚致死浓度,然后在玻璃水族箱内进行4个处理,3个重复,每个水族箱放养10尾鱼(1-3 g)。处理分别为5、6.3、8.06和10.23 mg L-1氯化钠和1、1.56、2.45和3.83 mg L-1甲基硫代氯化钠。每组设1个对照组,不添加任何化学物质。处理96 h后,进行微生物和组织病理学检查。在鱼鳃上观察到出血、次级片伸长、次级片粘连、支撑软骨肥大、粘液凝固分泌、片层增生、片层坏死和鳃片棒状。肝脏还可见胆道抑制、出血、细胞坏死和脂质变性。这些影响的严重程度是剂量依赖性的。只有亚致死浓度的甲基硫鎓氯显著影响皮肤上的细菌负荷(CFU g-1),而氯化钠和甲基硫鎓氯的其他处理没有表现出任何显著影响。综上所述,亚致死剂量甲基硫代氯化铵和氯化钠对波斯鲟鱼种鳃、皮肤及周围水体的消毒效果不明显。然而,在所有治疗组的鱼鳃、皮肤和肝脏均观察到组织病理学改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
caspian journal of environmental sciences
caspian journal of environmental sciences Environmental Science-Environmental Science (all)
CiteScore
2.30
自引率
0.00%
发文量
0
审稿时长
5 weeks
期刊最新文献
Biochemical responses of some aquatic plants as indicators for the treatment of inorganic nitrogen compounds in wastewater (Case study: Domestic water treatment plant in Babil Governorate, Iraq) Influence of nitrogen fertilizers on protein productivity of vetch-wheat grain under different water supply conditions Institutional mechanisms for unrelated agricultural support in the Russian Federation and the Federal Republic of Germany Energy and economic efficiency of corn silage production with flat grain of soy bean on reclaimed lands of upper volga Features of environmentally-friendly product obtaining from sugar and fodder beets in conditions of zinc contamination of sod-podzolic sandy loam soils
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1