{"title":"AN ELECTROCHEMILUMINESCENCE SANDWICH BIOSENSOR FOR THE DETECTION OF LIPOPOLYSACCHARIDE","authors":"Xuemei Fan, Zhejian Li, Shumin Wang, Yimeng Wang, Ling-min Yu, Xin-hui Fan","doi":"10.21577/0100-4042.20170548","DOIUrl":null,"url":null,"abstract":"A novel electrochemiluminescence (ECL) biosensor for the determination of lipopolysaccharide (LPS), a biomarker of endotoxemia, was designed. The nanoprobe (Ru1@SiO2-peptide) with a specific peptide (CIGKLHSAGK) labeled with a carboxyl functionalized Ru@SiO2 (Ru1@SiO2) was prepared by the acylation reaction. The ECL peptide biosensor was obtained by the strong gold-sulfur bond between Au on the gold electrode (AuE) surface and thiol group in the peptide. In the present of LPS, the peptide on the ECL biosensor can capture effectively LPS and the LPS/peptide/AuE was fabricated. When the Ru1@SiO2-peptide nanoprobe was bound, a sandwich compound of Ru1@SiO2-peptide/LPS/peptide was formed, and Ru(bpy)3, in the sandwich compound, will do the ECL performance. The effective ECL amplification of Ru1@SiO2 exhibited excellent analytical performance to LPS with a wide detection linear range of 1.0~500 ng mL and the detection limit was 0.3 ng mL. The biosensor could provide an evaluate of LPS expression on the bacteria surface and guide a new path to the clinical analysis of LPS related diseases.","PeriodicalId":49641,"journal":{"name":"Quimica Nova","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Quimica Nova","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.21577/0100-4042.20170548","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
Abstract
A novel electrochemiluminescence (ECL) biosensor for the determination of lipopolysaccharide (LPS), a biomarker of endotoxemia, was designed. The nanoprobe (Ru1@SiO2-peptide) with a specific peptide (CIGKLHSAGK) labeled with a carboxyl functionalized Ru@SiO2 (Ru1@SiO2) was prepared by the acylation reaction. The ECL peptide biosensor was obtained by the strong gold-sulfur bond between Au on the gold electrode (AuE) surface and thiol group in the peptide. In the present of LPS, the peptide on the ECL biosensor can capture effectively LPS and the LPS/peptide/AuE was fabricated. When the Ru1@SiO2-peptide nanoprobe was bound, a sandwich compound of Ru1@SiO2-peptide/LPS/peptide was formed, and Ru(bpy)3, in the sandwich compound, will do the ECL performance. The effective ECL amplification of Ru1@SiO2 exhibited excellent analytical performance to LPS with a wide detection linear range of 1.0~500 ng mL and the detection limit was 0.3 ng mL. The biosensor could provide an evaluate of LPS expression on the bacteria surface and guide a new path to the clinical analysis of LPS related diseases.
设计了一种新的电化学发光(ECL)生物传感器,用于检测内毒素血症的生物标志物脂多糖(LPS)。通过酰化反应制备了具有羧基功能化Ru@SiO2 (Ru1@SiO2)标记的特异性肽(CIGKLHSAGK)的纳米探针(Ru1@SiO2-peptide)。ECL肽生物传感器是利用金电极(AuE)表面的Au与肽中的硫基之间的强金-硫键获得的。在LPS存在的情况下,ECL生物传感器上的肽能有效捕获LPS,制备了LPS/肽/AuE。当Ru1@SiO2-peptide纳米探针结合时,形成Ru1@SiO2-peptide/LPS/肽的夹心化合物,夹心化合物中的Ru(bpy)3将发挥ECL性能。Ru1@SiO2的ECL有效扩增对LPS具有良好的分析性能,检测线性范围为1.0~500 ng mL,检出限为0.3 ng mL。该生物传感器可评价细菌表面LPS的表达情况,为临床分析LPS相关疾病提供新的途径。
期刊介绍:
Química Nova publishes in portuguese, spanish and english, original research articles, revisions, technical notes and articles about education in chemistry. All the manuscripts submitted to QN are evaluated by, at least, two reviewers (from Brazil and abroad) of recognized expertise in the field of chemistry involved in the manuscript. The Editorial Council can be eventually asked to review manuscripts. Editors are responsible for the final edition of QN.