ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY Quimica Nova Pub Date : 2023-10-27 DOI:10.21577/0100-4042.20230071

S. Ansari, Rukhsana Satar, N. Ikram, M. Awais

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Abstract

The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size and spherical in shape. Excellent biocompatibility of sodium alginate resulted in 90% immobilization yield for β-galactosidase due to covalent attachment. Soluble and immobilized β-galactosidase exhibited its pH and temperature-optima at pH 7.0 and at 40 oC, respectively. However, the enzyme attached to ACMONPs enhanced its activity at higher and lower pH and temperature ranges, in contrast to enzyme in solution. ACMONPs bound enzyme displayed greater enzyme activity under the effect of galactose mediated product inhibition as well as in reusability experiment. Our study indicated that 88% lactose was hydrolyzed by β-galactosidase immobilized on ACMONPs at 40 oC as compared to 71% by soluble enzyme under identical temperature in controlled batch reactors. Hence, this nanomatrix can find its potential application in continuous packed-bed reactors for obtaining lactose-free dairy products.

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海藻酸盐包被的MgO-NPs提高了克鲁维酵母β-半乳糖苷酶的稳定性，可用于生物技术应用

本研究旨在将乳酸克鲁维酵母β-半乳糖苷酶固定在海藻酸盐包覆的氧化镁纳米颗粒(ACMONPs)上。透射电镜显示湿法合成的MgO-NPs尺寸为27 nm，呈球形。海藻酸钠具有良好的生物相容性，通过共价吸附使β-半乳糖苷酶的固定化率达到90%。可溶性β-半乳糖苷酶和固定化β-半乳糖苷酶的最适pH值为7.0，最适温度为40℃。然而，与溶液中的酶相比，附着在ACMONPs上的酶在较高和较低pH和温度范围内的活性增强。ACMONPs结合酶在半乳糖介导的产物抑制作用和重复利用实验中表现出更强的酶活性。我们的研究表明，在40℃时，固定在ACMONPs上的β-半乳糖苷酶对乳糖的水解率为88%，而在控制的间歇反应器中，相同温度下可溶性酶对乳糖的水解率为71%。因此，该纳米基质可以在连续填充床反应器中找到其潜在的应用，以获得无乳糖乳制品。

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来源期刊

Quimica Nova 化学-化学综合

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

2-4 weeks

期刊介绍： Química Nova publishes in portuguese, spanish and english, original research articles, revisions, technical notes and articles about education in chemistry. All the manuscripts submitted to QN are evaluated by, at least, two reviewers (from Brazil and abroad) of recognized expertise in the field of chemistry involved in the manuscript. The Editorial Council can be eventually asked to review manuscripts. Editors are responsible for the final edition of QN.