Gene flow and genetic diversity in Consolida (Ranunculaceae) using sequence related amplified polymorphism

4区农林科学 Q3 Agricultural and Biological Sciences Genetika-Belgrade Pub Date : 2022-01-01 DOI:10.2298/gensr2203183a

N. Abdulla, S. Hamarashid, Syamand A. Qadir, A. Faraj, S. Tobakari

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Abstract

The genus Consolida (DC.) Gray (Ranuculaceae) belongs to tribe Delphinieae. It comprises approximately 52 species, including the members of the genus Aconitella Spach. Iraq is one of the richest countries for the genus in South-West Asia. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Consolida species, were studied through a molecular data. Seventy individuals related to five Consolida were collected in 5 provinces. A total of 75 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of five Consolida species. These bands were produced with the combinations of 5 selective primers. Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Consolida species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iraq.

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利用序列相关扩增多态性分析毛茛属植物的基因流动和遗传多样性

牛蒡属(DC.)灰飞燕属飞燕科。它包括大约52种，包括乌头属的成员。伊拉克是西南亚最富有的国家之一。通过序列相关扩增多态性评估遗传多样性。为了揭示固结属植物的遗传多样性和物种特征，本文对固结属植物进行了分子数据研究。在5个省共收集到5种固形菌属70个个体。通过聚合酶链反应(PCR)对5种固形菌进行扩增，共扩增出75条(NTL) DNA条带。这些条带由5条选择性引物组合而成。结果表明，序列相关的扩增多态性具有鉴定和破译固形莲属植物遗传亲和力的潜力。目前的结果对生物多样性和保护计划具有启示意义。除此之外，目前的结果可以为在伊拉克选择合适的草料和牧场生态类型铺平道路。

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.