D. Urošević, D. Knežević, Gordana Branković, A. Novoselskaya-Dragovich, A. Kudryavtsev, Mirela Matković-Stojšin, D. Mićanović, V. Zečević
The aim of this study was determination of protein content and amino acid composition in seeds of bread wheat, with particular focus of evaluation essential amino acids (EAAs). For analysis used flour samples of grained seed of 10 wheat variety, which selected in different breeding center (in Novi Sad and Kragujevac, Serbia). Kjeldahl method was used for determination of nitrogen (N) contents which value multiplied with coefficient 5.7 for computing protein content (protein contents = 5.7 x % N contents). Amino acids analyses of wheat samples were performed by ion exchange chromatography, followed by the ninhydrin colour reaction and photometric detection at 570 nm and 440 nm (for proline). The results showed that the mean protein content for wheat varied from the lowest value 10.24% in Ljubicevka to the highest 14.21% in Fortuna variety. The mean contents (g 100 g-l protein) of nonessential amino acids (NEAAs) were aspartic acid 5.42%, serine 4.23%, glutamic acid 18.51%, proline 12.18%, glycine 4.17%, alanine 3.64%, tyrosine 2.52%, arginine 5.02%, while mean contents of essential amino acids (EAAs) were threonine 2.86%, valine 4.52%, methionine 1.28%, isoleucine 3.87%, leucine 5.87%, phenylalanine 4.62%, histidine 2.46%, lysine 2.91%. The wheat variety contained relatively high concentration of the most deficient EAAs. The variety Ljubicevka contained relatively high concentration of the most deficient EAAs (threonine, valine, leucine, lysine,). The highest value (29.31 g 100 g-l protein) of total essential amino acids (TEAA), 59.79 g 100 g-l protein of total nonessential amino acids (TNEAA) as well as the highest value (90.35 g 100 g-l protein) of total amino acids (TAA). Obtained results provide variability of wheat variety on the base of amino acid contents and indicate reliable variety for selecting desirable parents in breeding program for improving nutrient quality.
本研究旨在测定面包小麦种子的蛋白质含量和氨基酸组成，重点研究必需氨基酸(EAAs)的含量。为了进行分析，使用了在不同育种中心(诺维萨德和塞尔维亚克拉古耶瓦茨)选择的10个小麦品种的颗粒种子的面粉样品。用凯氏定氮法测定氮含量，其值乘以系数5.7计算蛋白质含量(蛋白质含量= 5.7 x % N含量)。采用离子交换色谱法对小麦样品进行氨基酸分析，然后进行茚三酮显色反应和570 nm和440 nm光度检测(脯氨酸)。结果表明，小麦的平均蛋白质含量从柳比采夫卡最低的10.24%到福图纳最高的14.21%不等。非必需氨基酸(neaa)的平均含量(g 100 g-l蛋白)为天冬氨酸5.42%、丝氨酸4.23%、谷氨酸18.51%、脯氨酸12.18%、甘氨酸4.17%、丙氨酸3.64%、酪氨酸2.52%、精氨酸5.02%;必需氨基酸(eaa)的平均含量为苏氨酸2.86%、缬氨酸4.52%、蛋氨酸1.28%、异亮氨酸3.87%、亮氨酸5.87%、苯丙氨酸4.62%、组氨酸2.46%、赖氨酸2.91%。该小麦品种具有较高的最缺eaa浓度。品种Ljubicevka中最缺乏的eaa(苏氨酸、缬氨酸、亮氨酸、赖氨酸)含量相对较高。总必需氨基酸(TEAA)的最大值为29.31 g 100 g-l蛋白，总非必需氨基酸(TNEAA)的最大值为59.79 g 100 g-l蛋白，总氨基酸(TAA)的最大值为90.35 g 100 g-l蛋白。所得结果为小麦品种氨基酸含量的变异性提供了依据，为提高小麦营养品质的育种计划中选择理想亲本提供了可靠的品种。
{"title":"Protein content and amino acid composition in seed of bread wheat (Triticum aestivum L.)","authors":"D. Urošević, D. Knežević, Gordana Branković, A. Novoselskaya-Dragovich, A. Kudryavtsev, Mirela Matković-Stojšin, D. Mićanović, V. Zečević","doi":"10.2298/gensr2301301u","DOIUrl":"https://doi.org/10.2298/gensr2301301u","url":null,"abstract":"The aim of this study was determination of protein content and amino acid composition in seeds of bread wheat, with particular focus of evaluation essential amino acids (EAAs). For analysis used flour samples of grained seed of 10 wheat variety, which selected in different breeding center (in Novi Sad and Kragujevac, Serbia). Kjeldahl method was used for determination of nitrogen (N) contents which value multiplied with coefficient 5.7 for computing protein content (protein contents = 5.7 x % N contents). Amino acids analyses of wheat samples were performed by ion exchange chromatography, followed by the ninhydrin colour reaction and photometric detection at 570 nm and 440 nm (for proline). The results showed that the mean protein content for wheat varied from the lowest value 10.24% in Ljubicevka to the highest 14.21% in Fortuna variety. The mean contents (g 100 g-l protein) of nonessential amino acids (NEAAs) were aspartic acid 5.42%, serine 4.23%, glutamic acid 18.51%, proline 12.18%, glycine 4.17%, alanine 3.64%, tyrosine 2.52%, arginine 5.02%, while mean contents of essential amino acids (EAAs) were threonine 2.86%, valine 4.52%, methionine 1.28%, isoleucine 3.87%, leucine 5.87%, phenylalanine 4.62%, histidine 2.46%, lysine 2.91%. The wheat variety contained relatively high concentration of the most deficient EAAs. The variety Ljubicevka contained relatively high concentration of the most deficient EAAs (threonine, valine, leucine, lysine,). The highest value (29.31 g 100 g-l protein) of total essential amino acids (TEAA), 59.79 g 100 g-l protein of total nonessential amino acids (TNEAA) as well as the highest value (90.35 g 100 g-l protein) of total amino acids (TAA). Obtained results provide variability of wheat variety on the base of amino acid contents and indicate reliable variety for selecting desirable parents in breeding program for improving nutrient quality.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68298005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morteza Ghanadpour, Nezhad Kazemi, H. Galehdari, M. Hajjari
Gastric cancer is one of the most common cancers in the world. Delayed diagnosis is the most common cause of death in patients. Long noncoding RNAs (lncRNAs) are a group of non-coding RNAs that are effective in the incidence of cancers. Studies in different cancers determined HOTAIR as an important lncRNA in tumorigenesis. In gastric cancer, the function of HOTAIR in the initiation and progression of cancer seems to be crucial. In this study, we confirmed the significant differential expression of HOTAIR between gastric tumors and normal tissues in different datasets. In the following, the regulatory function of HOTAIR and its interaction with miRNAs in development of gastric cancer was analyzed. Our analysis determined that the upregulation of HOTAIR is essential to different pathways associated with the progression of gastric cancer. Further analysis determined numerous miRNAs as potential targets for HOTAIR. Among them, we demonstrated miR-1 as a significant miRNA with negative correlation with HOTAIR in gastric tumors. Validation analysis determined that HOTAIR is a target of cisplatin as a common chemotherapy drug. Eventually, the effect of cisplatin on the expression of HOTAIR and its potential target, miR-1, was checked by an in vitro study. Cisplatin treatment on the gastric cancer cell line showed that there is a significant negative correlation between the downregulation of HOTAIR and the upregulation of miR-1 in treated cells. In conclusion, comprehensive in silico analysis and experimental study provided evidence for the importance of the HOTAIR/miR-1 axis as potential diagnostic and treatment strategies for gastric cancer.
{"title":"HOTAIR/miR1 axis acts as a potential chemotherapy target in gastric cancer","authors":"Morteza Ghanadpour, Nezhad Kazemi, H. Galehdari, M. Hajjari","doi":"10.2298/gensr2301071g","DOIUrl":"https://doi.org/10.2298/gensr2301071g","url":null,"abstract":"Gastric cancer is one of the most common cancers in the world. Delayed diagnosis is the most common cause of death in patients. Long noncoding RNAs (lncRNAs) are a group of non-coding RNAs that are effective in the incidence of cancers. Studies in different cancers determined HOTAIR as an important lncRNA in tumorigenesis. In gastric cancer, the function of HOTAIR in the initiation and progression of cancer seems to be crucial. In this study, we confirmed the significant differential expression of HOTAIR between gastric tumors and normal tissues in different datasets. In the following, the regulatory function of HOTAIR and its interaction with miRNAs in development of gastric cancer was analyzed. Our analysis determined that the upregulation of HOTAIR is essential to different pathways associated with the progression of gastric cancer. Further analysis determined numerous miRNAs as potential targets for HOTAIR. Among them, we demonstrated miR-1 as a significant miRNA with negative correlation with HOTAIR in gastric tumors. Validation analysis determined that HOTAIR is a target of cisplatin as a common chemotherapy drug. Eventually, the effect of cisplatin on the expression of HOTAIR and its potential target, miR-1, was checked by an in vitro study. Cisplatin treatment on the gastric cancer cell line showed that there is a significant negative correlation between the downregulation of HOTAIR and the upregulation of miR-1 in treated cells. In conclusion, comprehensive in silico analysis and experimental study provided evidence for the importance of the HOTAIR/miR-1 axis as potential diagnostic and treatment strategies for gastric cancer.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muchlish Adie, A. Arifin, Suyamto Suyamto, A. Krisnawati
Soybeans with small seed sizes and high seed yield potential are crucial for addressing the needs of soybean-based industries. The objectives of the study were to investigate the maternal effect and quantify the value of heterosis, heterobeltiosis, and gene action in F1 plants from soybean crosses with small-seeded. In the present investigation, the experiment was conducted in the screen house of the Indonesian Legume and Tubers Crop Research Institute (ILETRI) located in Malang (East Java, Indonesia) from March to July 2021. The F1 plants were derived from crosses of Lokal Jember ? Tidar, Tidar ? Gepak Kuning, Gepak Kuning ? Detam 3, and their reciprocals. The parental genotypes and F1 plants were all planted. The observed data were plant height, number of branches, number of nodes, number of filled pods, the weight of 10 seeds, and seed yield/plant. The performance of the F1 plants showed variation for all observed traits, which is mostly averaged between the two parents, including 10 seed weight and seed yield. There was no maternal effect for almost all agronomic traits on all crosses and their reciprocals, except for the number of nodes in the cross of Gepak Kuning ? Detam 3. Heterosis (hMP) and heterobeltiosis (hHP) of F1 derived from six cross combinations for seven agronomic traits ranged from -25.93 to 186.27 and -94.83 to 114.71, respectively. Based on the potence ratio, the gene action was differed from a cross to cross for seed yield and its components. The degree of dominance for seed size and seed yield varied from overdominance, incomplete dominance, and complete dominance. Thus, based on the present investigation, it is possible to develop soybean varieties with small seed sizes and high yield by using pedigree selection on F2.
{"title":"Estimation of heterosis, heterobeltiosis, and gene action in F1 plants from small-seeded soybean crosses","authors":"Muchlish Adie, A. Arifin, Suyamto Suyamto, A. Krisnawati","doi":"10.2298/gensr2301203a","DOIUrl":"https://doi.org/10.2298/gensr2301203a","url":null,"abstract":"Soybeans with small seed sizes and high seed yield potential are crucial for addressing the needs of soybean-based industries. The objectives of the study were to investigate the maternal effect and quantify the value of heterosis, heterobeltiosis, and gene action in F1 plants from soybean crosses with small-seeded. In the present investigation, the experiment was conducted in the screen house of the Indonesian Legume and Tubers Crop Research Institute (ILETRI) located in Malang (East Java, Indonesia) from March to July 2021. The F1 plants were derived from crosses of Lokal Jember ? Tidar, Tidar ? Gepak Kuning, Gepak Kuning ? Detam 3, and their reciprocals. The parental genotypes and F1 plants were all planted. The observed data were plant height, number of branches, number of nodes, number of filled pods, the weight of 10 seeds, and seed yield/plant. The performance of the F1 plants showed variation for all observed traits, which is mostly averaged between the two parents, including 10 seed weight and seed yield. There was no maternal effect for almost all agronomic traits on all crosses and their reciprocals, except for the number of nodes in the cross of Gepak Kuning ? Detam 3. Heterosis (hMP) and heterobeltiosis (hHP) of F1 derived from six cross combinations for seven agronomic traits ranged from -25.93 to 186.27 and -94.83 to 114.71, respectively. Based on the potence ratio, the gene action was differed from a cross to cross for seed yield and its components. The degree of dominance for seed size and seed yield varied from overdominance, incomplete dominance, and complete dominance. Thus, based on the present investigation, it is possible to develop soybean varieties with small seed sizes and high yield by using pedigree selection on F2.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Simple Sequence Repeat (SSR) markers were used to assess the genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) respectively. Experiment was carried out at the Experimental Research Farm of the Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (HP) during the years 2015 and 2016. Experimental material used in the study comprised of Khira-75, a commercial variety grown in low and mid hills of the state (Himachal Pradesh) which is late in flowering and genotype PI-618860, an inbred line collected from North Central Regional Plant Introduction Station, USA which is early in flowering and tolerates various biotic stresses. For assessment of genetic purity and earliness the parents Khira-75 and PI-618860 were crossed and F1 and F2 population were raised. Genetic purity studies revealed that one marker CSN 160 was observed to be segregating in the parents as well as the population. Further, scoring of earliness in F2 population revealed promising results using 2 co-dominant SSR00262 and SSR17922 markers, which segregated in the parents and population in correlation with the observed phenotypes.
利用SSR (Simple Sequence Repeat)标记对黄瓜(Cucumis sativus L.) F1和F2群体的遗传纯度和早熟性进行了评价。实验于2015年和2016年在喜马偕尔邦索兰瑙尼的Yashwant Singh Parmar博士园艺和林业大学蔬菜科学系的实验研究农场进行。研究中使用的实验材料包括kira -75和PI-618860, kira -75是一种生长在喜马偕尔邦(Himachal Pradesh)低山丘和中山的商业品种，开花晚，基因型PI-618860是一种从美国中北部地区植物引种站收集的自交系，开花早，耐各种生物胁迫。为评价遗传纯度和早熟性，将亲本Khira-75与PI-618860杂交，饲养F1和F2群体。遗传纯度研究表明，一个标记csn160在亲本和群体中均有分离。此外，利用ssr262和SSR17922两种共显性标记对F2群体进行早期评分显示了令人满意的结果，这些标记在亲本和群体中分离，与观察到的表型相关。
{"title":"Assessment of genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) using SSR markers","authors":"M. Thakur, Ramesh Kumar, Parul Sharma, R. Sharma","doi":"10.2298/gensr2301033t","DOIUrl":"https://doi.org/10.2298/gensr2301033t","url":null,"abstract":"Simple Sequence Repeat (SSR) markers were used to assess the genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) respectively. Experiment was carried out at the Experimental Research Farm of the Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (HP) during the years 2015 and 2016. Experimental material used in the study comprised of Khira-75, a commercial variety grown in low and mid hills of the state (Himachal Pradesh) which is late in flowering and genotype PI-618860, an inbred line collected from North Central Regional Plant Introduction Station, USA which is early in flowering and tolerates various biotic stresses. For assessment of genetic purity and earliness the parents Khira-75 and PI-618860 were crossed and F1 and F2 population were raised. Genetic purity studies revealed that one marker CSN 160 was observed to be segregating in the parents as well as the population. Further, scoring of earliness in F2 population revealed promising results using 2 co-dominant SSR00262 and SSR17922 markers, which segregated in the parents and population in correlation with the observed phenotypes.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68296911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Marjanovic, Aleksa Palibrk, V. Dobričić, Ognjen Milićević, M. Branković, Vanja Viric, Aleksandra Drinic, Gorana Mandić-Stojmenović, M. Janković, I. Basta, S. Perić, I. Novaković, E. Stefanova, Z. Stevic
Hexanucleotide repeats expansion in the C9orf72 gene is the most common cause of familial and sporadic amyotrophic lateral sclerosis (ALS) cases in Europe. In this study we aimed to determine the size and distribution of C9orf72 alleles, and investigate the possible association of the repeat size with several clinical parameters in ALS patients from Serbia. Patients were recruited from 2011-2021 and analysed using fragment length analysis and Southern blot. Out of 383 ALS patients, we have detected 31 (8.09%) patients with repeat expansion. In the total ALS cohort, clinical overlap with frontotemporal dementia (FTD) was registered in 17 (4.44%) patients, and among them, 5 (29.41%) were expansion carriers. There was no difference in the age of onset, age at the examination or disease duration, gender, and the frequency of spinal and bulbar onset between patients with and without C9orf72 expansion. The presence of positive family history (34.48% vs. 15.65%) and FTD (16.13% vs. 3.41%) was more frequent in expansion-positive vs. expansion-negative patients. In expansion-positive patients, significantly higher values of the largest detected repeat were found in patients with ALS in the family, and in expansion-negative, a higher median value of the smaller allele was noted in patients with a positive family history of ALS, dementia, and both in comparison to the rest of the group. A correlation of the repeat size was not found with the age of onset in both patients with and without the expansion. This is the first detailed study of C9orf72 sizing in ALS patients from Serbia. Our results emphasize the need for C9orf72 genetic screening in ALS patients with/without FTD.
{"title":"C9orf72 genetic screening in amyotrophic lateral sclerosis patients from Serbia","authors":"A. Marjanovic, Aleksa Palibrk, V. Dobričić, Ognjen Milićević, M. Branković, Vanja Viric, Aleksandra Drinic, Gorana Mandić-Stojmenović, M. Janković, I. Basta, S. Perić, I. Novaković, E. Stefanova, Z. Stevic","doi":"10.2298/gensr2301001m","DOIUrl":"https://doi.org/10.2298/gensr2301001m","url":null,"abstract":"Hexanucleotide repeats expansion in the C9orf72 gene is the most common cause of familial and sporadic amyotrophic lateral sclerosis (ALS) cases in Europe. In this study we aimed to determine the size and distribution of C9orf72 alleles, and investigate the possible association of the repeat size with several clinical parameters in ALS patients from Serbia. Patients were recruited from 2011-2021 and analysed using fragment length analysis and Southern blot. Out of 383 ALS patients, we have detected 31 (8.09%) patients with repeat expansion. In the total ALS cohort, clinical overlap with frontotemporal dementia (FTD) was registered in 17 (4.44%) patients, and among them, 5 (29.41%) were expansion carriers. There was no difference in the age of onset, age at the examination or disease duration, gender, and the frequency of spinal and bulbar onset between patients with and without C9orf72 expansion. The presence of positive family history (34.48% vs. 15.65%) and FTD (16.13% vs. 3.41%) was more frequent in expansion-positive vs. expansion-negative patients. In expansion-positive patients, significantly higher values of the largest detected repeat were found in patients with ALS in the family, and in expansion-negative, a higher median value of the smaller allele was noted in patients with a positive family history of ALS, dementia, and both in comparison to the rest of the group. A correlation of the repeat size was not found with the age of onset in both patients with and without the expansion. This is the first detailed study of C9orf72 sizing in ALS patients from Serbia. Our results emphasize the need for C9orf72 genetic screening in ALS patients with/without FTD.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68296778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Genetic evaluation of segregating populations is one of the primary activities of cotton breeders. Seven cotton genotypes were crossed in a line ? tester mating design. The population of 12 F1, 12 F2 and their parents were tested in Randomized Complete Block Design with three replications at the Nazilli Cotton Research Institute of T?rkiye during the 2014/2015 cotton-growing season. The genetic variability was significant for all characters studied. ?2GCA/?2SCA indicated non-additive gene action in controlling seed cotton yield (F2), ginning out-turn (F1) and fiber quality traits for both generations. The highly favourable GCA effects for seed cotton yield and fiber strength were recorded in the Carmen cultivar. Carmen ? Carisma hybrid exhibited high mean performance and SCA effects for seed cotton yield and fiber strength, while the performance of Gloria ? ST-468 was superior in terms of ginning out-turn. We concluded that individual plants with desired traits should be selected in later generations due to the preponderance of non-additive gene actions.
{"title":"Estimating gene action and combining ability for yield and fiber quality in cotton (Gossypium hirsutum L.)","authors":"Şerife Balci, V. Çınar, A. Unay","doi":"10.2298/gensr2301061b","DOIUrl":"https://doi.org/10.2298/gensr2301061b","url":null,"abstract":"Genetic evaluation of segregating populations is one of the primary activities of cotton breeders. Seven cotton genotypes were crossed in a line ? tester mating design. The population of 12 F1, 12 F2 and their parents were tested in Randomized Complete Block Design with three replications at the Nazilli Cotton Research Institute of T?rkiye during the 2014/2015 cotton-growing season. The genetic variability was significant for all characters studied. ?2GCA/?2SCA indicated non-additive gene action in controlling seed cotton yield (F2), ginning out-turn (F1) and fiber quality traits for both generations. The highly favourable GCA effects for seed cotton yield and fiber strength were recorded in the Carmen cultivar. Carmen ? Carisma hybrid exhibited high mean performance and SCA effects for seed cotton yield and fiber strength, while the performance of Gloria ? ST-468 was superior in terms of ginning out-turn. We concluded that individual plants with desired traits should be selected in later generations due to the preponderance of non-additive gene actions.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sheidai, Sogol Allahverdi, Narges Anvari, Somayeh Khamene, M. Alavijeh, Marziyeh Ghoreishi, F. Jamshidi, G. Khosravani, N. Kalhor, F. Koohdar
X-chromosome and its independent loci recently gained importance in genetic investigations concerned with disease control-case studies, population genetic analyses, and phylogenetic investigations. Infertility and lack of normal reproduction are important issues in health. Aazoospermia is a kind of infertility in men whose semen contains no sperm and can affect about 1-20% of the male population. The majority of cases of spermatogenic failure in humans are idiopathic, and the underlying causes are postulated to be genetic. We analyze the potential association between seven X-Chromosome SNPs and the neighboring sequences with azoospermia in four ethnic populations in Iran. We also carried out population genetic analyses to produce data on genetic variability, population structure, gene flow, and time of divergence in these ethnic groups, for the first time. As the X-chromosome genes/SNPs are in homozygous condition, for the association study we used multivariate statistical methods, which revealed the potential role of variable sites close to the studied SNPs in male infertility. Haplotype groups were identified for each of the seven SNPs. The results showed gene flow among ethnic groups due to migration and inter-breeding and Bayesian analysis of sequence data suggested divergence time for ethnic populations to be between 2-30 KY.
{"title":"Text-11 snps and neighboring sequences role in causing male infertility in some ethnic groups of Iranian and their potential role in estimating divergence time","authors":"M. Sheidai, Sogol Allahverdi, Narges Anvari, Somayeh Khamene, M. Alavijeh, Marziyeh Ghoreishi, F. Jamshidi, G. Khosravani, N. Kalhor, F. Koohdar","doi":"10.2298/gensr2301229s","DOIUrl":"https://doi.org/10.2298/gensr2301229s","url":null,"abstract":"X-chromosome and its independent loci recently gained importance in genetic investigations concerned with disease control-case studies, population genetic analyses, and phylogenetic investigations. Infertility and lack of normal reproduction are important issues in health. Aazoospermia is a kind of infertility in men whose semen contains no sperm and can affect about 1-20% of the male population. The majority of cases of spermatogenic failure in humans are idiopathic, and the underlying causes are postulated to be genetic. We analyze the potential association between seven X-Chromosome SNPs and the neighboring sequences with azoospermia in four ethnic populations in Iran. We also carried out population genetic analyses to produce data on genetic variability, population structure, gene flow, and time of divergence in these ethnic groups, for the first time. As the X-chromosome genes/SNPs are in homozygous condition, for the association study we used multivariate statistical methods, which revealed the potential role of variable sites close to the studied SNPs in male infertility. Haplotype groups were identified for each of the seven SNPs. The results showed gene flow among ethnic groups due to migration and inter-breeding and Bayesian analysis of sequence data suggested divergence time for ethnic populations to be between 2-30 KY.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sajid Muhammad, S. Jadoon, F. Mohammad, Hussain Khalil, Sher Ahmad, Ullah Khan
Identification of high yielding relatively stable genotypes is an integral objective of plant breeding programs. Testing of genotypes across environments is required to determine yield stability of genotypes. The specific objective of the current study was to analyze genotype by environment interaction (GEI) for grain yield of 44 bread wheat recombinant inbred lines (RILs) and six check cultivars using additive main effect and multiplicative interaction (AMMI) model. Experiments were planted using alpha lattice design with two replicates in Peshawar (E-1 and E-3), Hangu (E-2 and E-4) and Kohat (E-5) Khyber Pakhtunkhwa province, Pakistan. Analysis of variance revealed significant differences among RILs for all traits while interactions due to genotype by environment were significant for all traits except days to emergence and 1000-grain weight. Significant GEI justified environment-specific as well as AMMI analysis to identify RILs with specific and wider adaptation. The AMMI analysis revealed that the first interaction principal component analysis (IPCA 1) captured 64% of GEI sum of squares while the second interaction principal component analysis (IPCA 2) explained 25.8% of the interaction sum of square. The AMMI biplot identified G30 as high yielding genotype followed by G19 and G49, whereas low yielding RILs were G13, G8 and G7. Similarly G30, being close to IPCA1 axis, was the most stable RIL with wider adaptability followed by G31 and G25. Based on AMMI stability value (ASV), RILs G18 (2.15), G5 (2.78), G27 (3.72), G44 (4.31), G25 (4.43), G42 (4.57), G43 (5.78), G11 (5.82), G1 (7.66) and G29 (7.81) were recognized in the given order of relative stability. Stability analysis identified G49 (Wafaq ? Ghaznavi-98-3) as high yielding stable genotype among RILs which can be commercialized after fulfilling procedural requirements.
{"title":"AMMI analysis for grain yield in bread wheat recombinant inbred lines","authors":"Sajid Muhammad, S. Jadoon, F. Mohammad, Hussain Khalil, Sher Ahmad, Ullah Khan","doi":"10.2298/gensr2301125m","DOIUrl":"https://doi.org/10.2298/gensr2301125m","url":null,"abstract":"Identification of high yielding relatively stable genotypes is an integral objective of plant breeding programs. Testing of genotypes across environments is required to determine yield stability of genotypes. The specific objective of the current study was to analyze genotype by environment interaction (GEI) for grain yield of 44 bread wheat recombinant inbred lines (RILs) and six check cultivars using additive main effect and multiplicative interaction (AMMI) model. Experiments were planted using alpha lattice design with two replicates in Peshawar (E-1 and E-3), Hangu (E-2 and E-4) and Kohat (E-5) Khyber Pakhtunkhwa province, Pakistan. Analysis of variance revealed significant differences among RILs for all traits while interactions due to genotype by environment were significant for all traits except days to emergence and 1000-grain weight. Significant GEI justified environment-specific as well as AMMI analysis to identify RILs with specific and wider adaptation. The AMMI analysis revealed that the first interaction principal component analysis (IPCA 1) captured 64% of GEI sum of squares while the second interaction principal component analysis (IPCA 2) explained 25.8% of the interaction sum of square. The AMMI biplot identified G30 as high yielding genotype followed by G19 and G49, whereas low yielding RILs were G13, G8 and G7. Similarly G30, being close to IPCA1 axis, was the most stable RIL with wider adaptability followed by G31 and G25. Based on AMMI stability value (ASV), RILs G18 (2.15), G5 (2.78), G27 (3.72), G44 (4.31), G25 (4.43), G42 (4.57), G43 (5.78), G11 (5.82), G1 (7.66) and G29 (7.81) were recognized in the given order of relative stability. Stability analysis identified G49 (Wafaq ? Ghaznavi-98-3) as high yielding stable genotype among RILs which can be commercialized after fulfilling procedural requirements.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Iličić, A. Jelušić, M. Blagojević, F. Bagi, K. Vrandečić, S. Stanković, Tatjana Popovic-Milovanovic
Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Sumadija) and plum (Krusedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
{"title":"Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia","authors":"R. Iličić, A. Jelušić, M. Blagojević, F. Bagi, K. Vrandečić, S. Stanković, Tatjana Popovic-Milovanovic","doi":"10.2298/gensr2301159i","DOIUrl":"https://doi.org/10.2298/gensr2301159i","url":null,"abstract":"Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Sumadija) and plum (Krusedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Sevindik, Makbule Bozkurt, M. Yilmaz, Ezginur Şenyüz, M. Paksoy
In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Ayd?n province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 ISSR primers were used to determine the molecular characterization among the populations. For cpDNA trnL intron amplification, trnC and trnD primers were used. In ISSR analysis, a total of 70 bands were obtained. The polymorphism rate was determined to be approximately 94.28%. According to the ISSR analysis, the UPGMA dendrogram consisted of three groups. For cpDNA trnL intron sequences, phylogenetic analyses were obtained along with genetic distances. For populations, cpDNA trnL intron sequences were determined between 454 and 472 bases. The maximum likelihood phylogenetic tree consist of two clades. In addition, cpDNA trnL intron sequences of some species (Chrysophthalmum Pulicaria Inula Jasonia Stenachaenium Carpesium Blumea Iphiona Limbarda Rhanterium Lifago Duhaldea Chiliadenus) of Asteraceae family from NCBI with Dittrichia viscosa populations phylogenetic tree was constructed. As a result of the study, it was determined that the polymorphism rate of Dittrichia viscosa populations obtained by ISSR-PCR was higher than the results obtained from trnL intron sequences.
{"title":"Molecular characterization of Dittrichia viscosa (L.) greuter (Asteraceae) populations revealed by ISSR markers and chloroplast (CPDNA) tRNL intron sequences","authors":"E. Sevindik, Makbule Bozkurt, M. Yilmaz, Ezginur Şenyüz, M. Paksoy","doi":"10.2298/gensr2301217s","DOIUrl":"https://doi.org/10.2298/gensr2301217s","url":null,"abstract":"In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Ayd?n province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 ISSR primers were used to determine the molecular characterization among the populations. For cpDNA trnL intron amplification, trnC and trnD primers were used. In ISSR analysis, a total of 70 bands were obtained. The polymorphism rate was determined to be approximately 94.28%. According to the ISSR analysis, the UPGMA dendrogram consisted of three groups. For cpDNA trnL intron sequences, phylogenetic analyses were obtained along with genetic distances. For populations, cpDNA trnL intron sequences were determined between 454 and 472 bases. The maximum likelihood phylogenetic tree consist of two clades. In addition, cpDNA trnL intron sequences of some species (Chrysophthalmum Pulicaria Inula Jasonia Stenachaenium Carpesium Blumea Iphiona Limbarda Rhanterium Lifago Duhaldea Chiliadenus) of Asteraceae family from NCBI with Dittrichia viscosa populations phylogenetic tree was constructed. As a result of the study, it was determined that the polymorphism rate of Dittrichia viscosa populations obtained by ISSR-PCR was higher than the results obtained from trnL intron sequences.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68297905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：