Aflatoxigenic and ochratoxigenic moulds and their toxins in tree nuts on Serbian market

Irena Rakic, G. Dimić, M. Škrinjar, S. Kocić-Tanackov
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Abstract

In this study, moulds and mycotoxins presence in different tree nuts were investigated. The results showed that all of the 25 samples were contaminated with moulds. Mean values of total mould count varied from 1-4.9 cfu per grain. The most frequent species in hazelnut samples were Rhizopus oryzae (32.2%) and Aspergillus niger (28.9%). In walnuts A. niger (75.6%), in cashews also A. niger (42.4%) while in pistachio samples Alternaria alternata (20.7%), and Cladosporium cladosporioides (20.7%) were the most dominant. Rhizopus oligosporus was the only identified species in all almond samples (100%). Using Enzyme Linked Immunosorbent Assay (ELISA), the presence of total aflatoxins and ochratoxin A was examinated. In all analyzed samples, levels of ochratoxin A were below the limit of detection. Total aflatoxins were detected only in walnut samples with average concentration of 7.1 ?g/kg.
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塞尔维亚市场上树坚果中的黄曲霉毒素和赭曲霉毒素
在这项研究中,霉菌和真菌毒素存在于不同的树坚果进行了调查。结果表明,25份样品均存在霉菌污染。霉菌总数的平均值在每粒1-4.9 cfu之间变化。榛子样品中最常见的菌种为米根霉(32.2%)和黑曲霉(28.9%)。核桃(75.6%)、腰果(42.4%)、开心果(20.7%)中以互花霉(20.7%)和枝孢霉(20.7%)最占优势。所有杏仁样品中鉴定出的菌种只有少孢根霉(100%)。采用酶联免疫吸附试验(ELISA)检测总黄曲霉毒素和赭曲霉毒素A的存在。在所有分析的样本中,赭曲霉毒素A的含量都低于检测限度。总黄曲霉毒素仅在核桃样品中检测到,平均浓度为7.1 g/kg。
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审稿时长
8 weeks
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