A. M. Jnied, M. Rashed, M. Sallam, A. Atta, A. E. Badawy
{"title":"ISOLATION AND CHARACTERIZATION OF ACROSIN GENE (Acr), EXON 5 IN THE BUCKS OF GOAT BREEDS","authors":"A. M. Jnied, M. Rashed, M. Sallam, A. Atta, A. E. Badawy","doi":"10.21608/EJGC.2013.10194","DOIUrl":null,"url":null,"abstract":"Three Egyptian (Baladi, Barki and Zaraibi) and one Syrian (Damascus) goat breeds were used to isolate and characterize acrosin gene, exon 5. A sample of ten bucks individuals from Zaraibi and Damascus breeds and five bucks individuals from Baladi and Barki breeds were randomly taken and DNA was extracted from each individual and analyzed with the acrosin specific primers. One band with a fragment size of a 378 bp in all individual samples for all breeds represent exon 5 of Acr gene was obtained. Sequence analysis showed that Baladi and Zaraibi breeds were identical and differ from sequences of Damascus and Barki breeds (who also identical) by one base pair number 266 (T in Baladi and Zaraibi breeds while it was C in Barki and Damascus breeds). The genetic analysis for Acr gene, exon 5 showed that this region was conserved among the studied goat breeds.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"393-404"},"PeriodicalIF":0.0000,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Genetics and Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EJGC.2013.10194","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Three Egyptian (Baladi, Barki and Zaraibi) and one Syrian (Damascus) goat breeds were used to isolate and characterize acrosin gene, exon 5. A sample of ten bucks individuals from Zaraibi and Damascus breeds and five bucks individuals from Baladi and Barki breeds were randomly taken and DNA was extracted from each individual and analyzed with the acrosin specific primers. One band with a fragment size of a 378 bp in all individual samples for all breeds represent exon 5 of Acr gene was obtained. Sequence analysis showed that Baladi and Zaraibi breeds were identical and differ from sequences of Damascus and Barki breeds (who also identical) by one base pair number 266 (T in Baladi and Zaraibi breeds while it was C in Barki and Damascus breeds). The genetic analysis for Acr gene, exon 5 showed that this region was conserved among the studied goat breeds.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
