Morpho-agronomic and chemical analysis as well as RAPD markers were used to determine the genetic diversity among three Egyptian genotypes of sweet potato; Abees, Mabrouka and Gendawy. The results revealed that there is a wide variation among the three genotypes in most morphological and agronomic characters in addition to the nutritional values. Gendawy genotype had the highest values for most agronomic and chemical traits compared to the other two genotypes; therefore it is considered a good source of agronomic and nutritional traits for breeding. Regarding molecular characterization, a total of nine RAPD primers were used to assess the genetic variability and relationships among the three sweet potato genotypes. A total of 146 amplified bands were generated from the nine primers with 52.74% polymorphism indicating high genetic variability. Cluster analysis revealed a close genetic relationship between Abees and Gendawy genotypes (similarity value of 0.718), while Mabrouka was the most distinct genotype. Results concluded that RAPD analysis could not be effective in separating genotypes according to their morphological, agronomic or chemical characters. In addition, characterization based on these conventional characters should be complemented with DNA-based molecular characterization to reveal genetic diversity in the three Egyptian sweet potato genotypes.
{"title":"GENETIC CHARACTERIZATION OF THREE EGYPTIAN SWEET POTATO GENOTYPES BASED ON MORPHO-AGRONOMIC AND MOLECULAR MARKERS","authors":"O. Galal, A. Gendy","doi":"10.21608/EJGC.2018.9198","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9198","url":null,"abstract":"Morpho-agronomic and chemical analysis as well as RAPD markers were used to determine the genetic diversity among three Egyptian genotypes of sweet potato; Abees, Mabrouka and Gendawy. The results revealed that there is a wide variation among the three genotypes in most morphological and agronomic characters in addition to the nutritional values. Gendawy genotype had the highest values for most agronomic and chemical traits compared to the other two genotypes; therefore it is considered a good source of agronomic and nutritional traits for breeding. Regarding molecular characterization, a total of nine RAPD primers were used to assess the genetic variability and relationships among the three sweet potato genotypes. A total of 146 amplified bands were generated from the nine primers with 52.74% polymorphism indicating high genetic variability. Cluster analysis revealed a close genetic relationship between Abees and Gendawy genotypes (similarity value of 0.718), while Mabrouka was the most distinct genotype. Results concluded that RAPD analysis could not be effective in separating genotypes according to their morphological, agronomic or chemical characters. In addition, characterization based on these conventional characters should be complemented with DNA-based molecular characterization to reveal genetic diversity in the three Egyptian sweet potato genotypes.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49664138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The antitumor action of propolis is of clinical interest because of the need for new anticancer treatment agents. The present investigation intended to extract and assess the chemical content, cytotoxic action, the growth inhibitory activity and anticancer capability of Egyptian propolis versus Chinese propolis. This was carried out using water extract (WE) and ethanolic extract (EE) on the human hepatocellular carcinoma (HEp-2) cell line and the loss of heterozygosity (LOH) assay of Drosophila melanogaster somatic cells against the direct genotoxicity of doxorubicin. EPWE, EPEE, CPWE and CPEE extracts analyzed by HPLC showed that there were sensible and various concentrations of phenolic compounds in both. Total phenolics were determined to be 18.83, 34.87, 39.29 and 180.89 g-1 by using EPWE, CPWE, EPEE and CPEE extracts, respectively. Chinese propolis ethanol extract (CPEE) have major concentrations of total phenolics and phenolic acids and contained high concentrations of rutin (188.90 g/mL). The study of the antiproliferative capacity of propolis extractors against HEp-2 cancer cell lines showed that all the studied propolis extracts induce suppression of cell growth except CPWE extract; it gave 100% cell viability. The great majority of the propolis are strongly cytotoxic against HEp-2 cell line with 500 μg/ml CPEE. Also, PEE is the most effective in inhibition of HEp-2 cell proliferation compared to PWE. In Drosophila assay, treatment with propolis extract and DOX carcinogenic agent led to a reduction in the frequency of recombination compared to the treatment with DOX alone either in the post- and pre-treatments. In general, PEE exhibited powerful anti-proliferative effects than PWE. The ethanol extract provided the highest protection against Doxorubicin (DOR) induced genotoxicity, a fact that supports their anti-cancer activity. The results demonstrate that PEE is a good source of a natural antitumor operator able to inhibit cancer cell proliferation.
{"title":"MODULATOR IMPACTS OF PROPOLIS EXTRACT AGAINST DOXORUBICIN MEDIATED CARCINOGENESIS ON HEPATOCELLULAR CARCINOMA AND Drosophila SOMATIC CELLS","authors":"Naglaa M. Ebeed, Sawsan M. Abdelmegeed","doi":"10.21608/EJGC.2018.9211","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9211","url":null,"abstract":"The antitumor action of propolis is of clinical interest because of the need for new anticancer treatment agents. The present investigation intended to extract and assess the chemical content, cytotoxic action, the growth inhibitory activity and anticancer capability of Egyptian propolis versus Chinese propolis. This was carried out using water extract (WE) and ethanolic extract (EE) on the human hepatocellular carcinoma (HEp-2) cell line and the loss of heterozygosity (LOH) assay of Drosophila melanogaster somatic cells against the direct genotoxicity of doxorubicin. EPWE, EPEE, CPWE and CPEE extracts analyzed by HPLC showed that there were sensible and various concentrations of phenolic compounds in both. Total phenolics were determined to be 18.83, 34.87, 39.29 and 180.89 g-1 by using EPWE, CPWE, EPEE and CPEE extracts, respectively. Chinese propolis ethanol extract (CPEE) have major concentrations of total phenolics and phenolic acids and contained high concentrations of rutin (188.90 g/mL). The study of the antiproliferative capacity of propolis extractors against HEp-2 cancer cell lines showed that all the studied propolis extracts induce suppression of cell growth except CPWE extract; it gave 100% cell viability. The great majority of the propolis are strongly cytotoxic against HEp-2 cell line with 500 μg/ml CPEE. Also, PEE is the most effective in inhibition of HEp-2 cell proliferation compared to PWE. In Drosophila assay, treatment with propolis extract and DOX carcinogenic agent led to a reduction in the frequency of recombination compared to the treatment with DOX alone either in the post- and pre-treatments. In general, PEE exhibited powerful anti-proliferative effects than PWE. The ethanol extract provided the highest protection against Doxorubicin (DOR) induced genotoxicity, a fact that supports their anti-cancer activity. The results demonstrate that PEE is a good source of a natural antitumor operator able to inhibit cancer cell proliferation.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"46 1","pages":"389-407"},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48083708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The cytochrome P450 (CYP) superfamily of heme-containing enzymes catalyzes Phase I biotransformation of endogenous and xenobiotic compounds, including fatty acids, steroids, drugs, and environmental contaminants. Common variations (polymorphisms) in cytochrome P450 genes can affect the function of the enzymes. The effects of polymorphisms are most prominently seen in the breakdown of medications. In fish, members of the CYP2 and CYP3A families play a major role in the metabolism of xenobiotics and endogenous compounds. The current study aimed to isolate and compare cytochrome P450 3A40 gene from Oreochromis niloticus (Nile tilapia) to understand its diversity and evolution in comparison with fresh water fish species available in the GenBank database. Total length of 1300 bp was obtained and its polymorphism with similar samples from O. niloticus in the GenBank was determined. Site no. 41 T>A changed the amino acid from Phenylalanine (F) to Tyrosine (Y), while sites no. 43 and 54 caused no effect (silent mutations), and site no. 74 A>G changes the amino acid from Glutamic Acid (E) to Glycine (G), in which the two later mutations formed a different protein isoform in its conformational structure. Phylogenetic analysis reflected a clear divergence of fresh water families (Cichlidae and Poecilidae) from other families, while fixed and shared mutations between families were found. Phylogenetic analysis provided strong support for the identity of the majority of CYPs in fresh water fishes. In the current study, conservative regions among the studied families were found.
{"title":"DIVERSITY AND EVOLUTION OF CYP MITOCHONDRIAL GENE IN NILE TILAPIA (Oreochromis niloticus L.)","authors":"M. Rashed, Amira El-Kerady, Mahmoud Magdy","doi":"10.21608/EJGC.2018.9199","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9199","url":null,"abstract":"The cytochrome P450 (CYP) superfamily of heme-containing enzymes catalyzes Phase I biotransformation of endogenous and xenobiotic compounds, including fatty acids, steroids, drugs, and environmental contaminants. Common variations (polymorphisms) in cytochrome P450 genes can affect the function of the enzymes. The effects of polymorphisms are most prominently seen in the breakdown of medications. In fish, members of the CYP2 and CYP3A families play a major role in the metabolism of xenobiotics and endogenous compounds. The current study aimed to isolate and compare cytochrome P450 3A40 gene from Oreochromis niloticus (Nile tilapia) to understand its diversity and evolution in comparison with fresh water fish species available in the GenBank database. Total length of 1300 bp was obtained and its polymorphism with similar samples from O. niloticus in the GenBank was determined. Site no. 41 T>A changed the amino acid from Phenylalanine (F) to Tyrosine (Y), while sites no. 43 and 54 caused no effect (silent mutations), and site no. 74 A>G changes the amino acid from Glutamic Acid (E) to Glycine (G), in which the two later mutations formed a different protein isoform in its conformational structure. Phylogenetic analysis reflected a clear divergence of fresh water families (Cichlidae and Poecilidae) from other families, while fixed and shared mutations between families were found. Phylogenetic analysis provided strong support for the identity of the majority of CYPs in fresh water fishes. In the current study, conservative regions among the studied families were found.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47290512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Z. Ahmed, N. Rashed, A. Agorio, S. Filleur, N. Abdallah, A. Hemeida, M. Nasr
Egyptian native (nondomesticated) plant species from different families such as Capparis orientalis, Lycium shawii and Zygophyllum album were collected from North Western coast of Marsa Matrouh Governorate, Egypt. Plant vacuolar Na+/H+ antiporter candidate gene from tolerant plant species; several are localized on the tonoplast, plays an important role in several plant species (Halophytes and xerophytes) under abiotic stress. Then, once the genes will be identified from tolerant plant species, the overall goal of this study is to identify partial the vacuolar antiporter NHX1 candidate gene. According to NHX1 family homologous sequence conservative region; one degenerate oligonucleotide primer pair was used to amplify core (partial middle) fragment of cDNAs vacuolar Na+/H+ antiporter with about size of 600 bp, approximately. Touchdown PCR program (TD-PCR) of cDNAs were success to increase specificity, sensitivity and yield to amplify core cDNA of vacuolar Na+/H+ antiporter gene. Sequence analysis provided us with a novel partial fragment length of cDNAs about 548 bp, 557 bp and 557 bp of a novel CoNHX1, LsNHX and ZaNHX was deposited in GenBank database with NCBI, GenBank accession no. KJ452345.1, KJ452346.1 and KJ452347.1 and amino acid sequences about 182 a.a, 185 a.a and 185 a.a with GenBank accession no. AHY19036.1, AHY19037.1 and AHY19038.1, respectively. BLASTN of sequences result and phylogenetic relationship analysis indicated that all were clustered into the vacuolar Na+/H+ antiporter group. The deduced amino acid sequences showed high identities with other plant vacuolar-type Na+/H+ antiporters. Taken together, these results suggest that CoNHX, LsNHX and ZaNHX are new members of the vacuolar Na+/H+ antiporter family. The ultimate goal of this study provided a basic foundation information about a Novel partial vacuolar Na+/H+ antiporter gene to develop 5` and 3` RACE technique (Rapid amplification cDNA Ends).
{"title":"ISOLATION AND SEQUENCE ANALYSIS OF A NOVEL PARTIAL VACUOLAR Na+/H+ ANTIPORTER cDNA FROM Capparis orientalis, Lycium shawii AND Zygophyllum album","authors":"M. Z. Ahmed, N. Rashed, A. Agorio, S. Filleur, N. Abdallah, A. Hemeida, M. Nasr","doi":"10.21608/EJGC.2018.9194","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9194","url":null,"abstract":"Egyptian native (nondomesticated) plant species from different families such as Capparis orientalis, Lycium shawii and Zygophyllum album were collected from North Western coast of Marsa Matrouh Governorate, Egypt. Plant vacuolar Na+/H+ antiporter candidate gene from tolerant plant species; several are localized on the tonoplast, plays an important role in several plant species (Halophytes and xerophytes) under abiotic stress. Then, once the genes will be identified from tolerant plant species, the overall goal of this study is to identify partial the vacuolar antiporter NHX1 candidate gene. According to NHX1 family homologous sequence conservative region; one degenerate oligonucleotide primer pair was used to amplify core (partial middle) fragment of cDNAs vacuolar Na+/H+ antiporter with about size of 600 bp, approximately. Touchdown PCR program (TD-PCR) of cDNAs were success to increase specificity, sensitivity and yield to amplify core cDNA of vacuolar Na+/H+ antiporter gene. Sequence analysis provided us with a novel partial fragment length of cDNAs about 548 bp, 557 bp and 557 bp of a novel CoNHX1, LsNHX and ZaNHX was deposited in GenBank database with NCBI, GenBank accession no. KJ452345.1, KJ452346.1 and KJ452347.1 and amino acid sequences about 182 a.a, 185 a.a and 185 a.a with GenBank accession no. AHY19036.1, AHY19037.1 and AHY19038.1, respectively. BLASTN of sequences result and phylogenetic relationship analysis indicated that all were clustered into the vacuolar Na+/H+ antiporter group. The deduced amino acid sequences showed high identities with other plant vacuolar-type Na+/H+ antiporters. Taken together, these results suggest that CoNHX, LsNHX and ZaNHX are new members of the vacuolar Na+/H+ antiporter family. The ultimate goal of this study provided a basic foundation information about a Novel partial vacuolar Na+/H+ antiporter gene to develop 5` and 3` RACE technique (Rapid amplification cDNA Ends).","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46375041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Six Egyptian clover genotypes, i.e., Helaly, Sakha 4, Gemmeiza 1, Serw1, Giza6 and Sakha composite were used in this study. Data was calculated on four cuts and seasonal yield for fresh and dry forage yield. The results indicated that the mean square values were highly significant for genotypes for all studied traits. Helaly cultivar gave the highest values in four cuts and seasonal yield for fresh forage yield. High heritability values were recorded for all studied traits except 1st fresh yield which recorded moderate value. Phenotypic coefficient of variability (PCV) was higher than genotypic coefficient of variability (GCV) in all cuts and seasonal yields. Genetic variations among the six Egyptian clover cultivars were evaluated using universal rice primers (URP) and inter simple sequence repeat (ISSR) markers. URP fingerprinting detected more polymorphic loci (90%) than the ISSR fingerprinting (77.55%). Mean PIC (polymorphism information content) and band informativness (Ib) for each of these marker systems were (0.21, 0.26 for ISSR and 0.32, 0.46 for URP) respectively, suggested that URP marker systems were effective than ISSR in determining polymorphisms. Pairwise similarity index values ranged from 0.37 to 0.72 for ISSR, 0.23 to 0.62 for URP and 0.29 to 0.65 for combined ISSRs and URP indicated the genetic distinctness among the studied genotypes.
{"title":"ASSESSMENT OF GENETIC COMPONENTS AND GENETIC DIVERSITY OF SIX EGYPTIAN CLOVER (Trifolium alexandrinum L.) GENOTYPES USING ISSR AND URP MARKERS","authors":"A. El-Banna, M. Ghazy","doi":"10.21608/EJGC.2018.9206","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9206","url":null,"abstract":"Six Egyptian clover genotypes, i.e., Helaly, Sakha 4, Gemmeiza 1, Serw1, Giza6 and Sakha composite were used in this study. Data was calculated on four cuts and seasonal yield for fresh and dry forage yield. The results indicated that the mean square values were highly significant for genotypes for all studied traits. Helaly cultivar gave the highest values in four cuts and seasonal yield for fresh forage yield. High heritability values were recorded for all studied traits except 1st fresh yield which recorded moderate value. Phenotypic coefficient of variability (PCV) was higher than genotypic coefficient of variability (GCV) in all cuts and seasonal yields. Genetic variations among the six Egyptian clover cultivars were evaluated using universal rice primers (URP) and inter simple sequence repeat (ISSR) markers. URP fingerprinting detected more polymorphic loci (90%) than the ISSR fingerprinting (77.55%). Mean PIC (polymorphism information content) and band informativness (Ib) for each of these marker systems were (0.21, 0.26 for ISSR and 0.32, 0.46 for URP) respectively, suggested that URP marker systems were effective than ISSR in determining polymorphisms. Pairwise similarity index values ranged from 0.37 to 0.72 for ISSR, 0.23 to 0.62 for URP and 0.29 to 0.65 for combined ISSRs and URP indicated the genetic distinctness among the studied genotypes.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45172797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Non-Alcoholic fatty liver disease (NAFLD) is known as a common public health problem worldwide. Genetic variant as well as environmental factor interact to develop NAFLD. The allele frequency of PNPLA3 and GCKR variant varied in different ethnic groups. Our study aimed to recognize the association of the PNPLA3 rs738409 and GCKR rs1260326 with the occurrence and progression of NAFLD in obese Egyptian children. PNPLA3 rs738409 and GCKR rs1260326 were genotyped using RFLPPCR in 80 patients with NAFLD (40 with NAFL and 40 with non-alcoholic steatohepatitis [NASH]) and 80 control subjects. Comparison was made between NAFLD and control as well as between NAFL and NASH. The risk allele (G) allele frequency of PNPLA3 rs738409 was 0.456 in patients and 0.363 in control. Whereas, the risk allele (T) allele frequency of GCKR rs1260326 was 0.488 in patients and 0.331 in control. These two alleles show highly association with NAFLD (P = 0.00). Comparison between NAFL and NASH revealed that the frequencies of the (G) allele in PNPLA3 rs738409 were 0.3875, 0.5 in NAFL and NASH, respectively. Moreover, the allele frequencies of the (T) allele in GCKR rs1260326 were 0.413 and 0.575 in NAFL and NASH, respectively. Our findings demonstrate that both SNPs were highly associated with NASH. It is concluded that the PNPLA3 rs738409 and GCKR rs1260326 may have an important role in the development of NAFLD in obese Egyptian Children.
{"title":"ASSOCIATION OF PNPLA3 (rs738409) AND GCKR (rs1260326) GENE POLYMORPHISMS WITH THE DEVELOPMENT OF NONALCOHOLIC FATTY LIVER DISEASE IN OBESE EGYPTIAN CHILDREN","authors":"G. El-Nady, G. A. El-Fath","doi":"10.21608/EJGC.2018.9197","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9197","url":null,"abstract":"Non-Alcoholic fatty liver disease (NAFLD) is known as a common public health problem worldwide. Genetic variant as well as environmental factor interact to develop NAFLD. The allele frequency of PNPLA3 and GCKR variant varied in different ethnic groups. Our study aimed to recognize the association of the PNPLA3 rs738409 and GCKR rs1260326 with the occurrence and progression of NAFLD in obese Egyptian children. PNPLA3 rs738409 and GCKR rs1260326 were genotyped using RFLPPCR in 80 patients with NAFLD (40 with NAFL and 40 with non-alcoholic steatohepatitis [NASH]) and 80 control subjects. Comparison was made between NAFLD and control as well as between NAFL and NASH. The risk allele (G) allele frequency of PNPLA3 rs738409 was 0.456 in patients and 0.363 in control. Whereas, the risk allele (T) allele frequency of GCKR rs1260326 was 0.488 in patients and 0.331 in control. These two alleles show highly association with NAFLD (P = 0.00). Comparison between NAFL and NASH revealed that the frequencies of the (G) allele in PNPLA3 rs738409 were 0.3875, 0.5 in NAFL and NASH, respectively. Moreover, the allele frequencies of the (T) allele in GCKR rs1260326 were 0.413 and 0.575 in NAFL and NASH, respectively. Our findings demonstrate that both SNPs were highly associated with NASH. It is concluded that the PNPLA3 rs738409 and GCKR rs1260326 may have an important role in the development of NAFLD in obese Egyptian Children.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45624868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present work was carried out to examine the effect of exogenous application of stigmasterol on mitotic cell division; some growth parameters and protein banding pattern using two salt stressed Lupinus termis cultivars (cv. Giza 1 and Giza 2). On the other hand, the mutagenic and the antimutagenic effect of stigmasterol were studied using Allium cepa assay. The results of germination percentage revealed that cv. Giza 2 had higher response to the interaction between salinity and stigmasterol than cv. Giza 1. Also, highly significant inhibition in growth parameters (shoot and root growth) by increasing the salinity stress in Giza 1 than Giza 2 and this inhibition was significantly alleviated with stigmasterol treatment. Salinity induced a considerable variation in the protein patterns among these cultivars. These changes have been appeared in the novel expression of some polypeptides, the absence of the other and the over expression of a third class of polypeptides. Treatment with sodium chloride (100 and 200 Mm) has mitoclassic impact on cell division. Few types of mitotic abnormalities were induced in different treatments. Stigmasterol treatments were minimize the inhibition effect of NaCl and showed a considerable increase in the mitotic index. This study detected that stigmasterol has antimutagenic effect against sodium chloride that induced chromosomal aberrations in Allium cepa root meristematic cells.
{"title":"ANTIMUTAGENICS EFFECTS OF STIGMASTEROL ON TWO SALT STRESSED Lupinus termis CULTIVARS","authors":"H. Mahfouz, Walaa A. Rayan","doi":"10.21608/EJGC.2018.9196","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9196","url":null,"abstract":"The present work was carried out to examine the effect of exogenous application of stigmasterol on mitotic cell division; some growth parameters and protein banding pattern using two salt stressed Lupinus termis cultivars (cv. Giza 1 and Giza 2). On the other hand, the mutagenic and the antimutagenic effect of stigmasterol were studied using Allium cepa assay. The results of germination percentage revealed that cv. Giza 2 had higher response to the interaction between salinity and stigmasterol than cv. Giza 1. Also, highly significant inhibition in growth parameters (shoot and root growth) by increasing the salinity stress in Giza 1 than Giza 2 and this inhibition was significantly alleviated with stigmasterol treatment. Salinity induced a considerable variation in the protein patterns among these cultivars. These changes have been appeared in the novel expression of some polypeptides, the absence of the other and the over expression of a third class of polypeptides. Treatment with sodium chloride (100 and 200 Mm) has mitoclassic impact on cell division. Few types of mitotic abnormalities were induced in different treatments. Stigmasterol treatments were minimize the inhibition effect of NaCl and showed a considerable increase in the mitotic index. This study detected that stigmasterol has antimutagenic effect against sodium chloride that induced chromosomal aberrations in Allium cepa root meristematic cells.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42638813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Aboelenin, K. Mahrous, Amira El-Kerady, M. Rashed
Cytochrome P450 aromatase (CYP19) gene catalyzes the last step in the steroidogenesis which convert the androgens to estrogens. Therefore, the objective of this study was to investigate the polymorphism in CYP19 gene and its potential effects in female buffaloes fertility. The DNA was extracted from the blood samples of 81 Egyptian river buffalo females and a 419 bp fragment contained a part of CYP19- distal promoter P1.1 was amplified by PCR which subsequently treated with PvuII restriction enzyme. The PCR-RFLP pattern showed that all the animals had a fixed GG genotype and A allele was not detected. Sequencing of the amplified fragment (GenBank accession No. MF490278 and MF490279) followed by sequence alignment with the GenBank database revealed that the homology among the Egyptian river buffalo target sequence and its orthologues sequences in cattle, sheep and goat was 97, 95 and 93%, respectively. G to A transition SNP (G197A) was detected among individuals representing the Egyptian river buffalo by sequencing. G allele was detected only in the Egyptian buffalo and not in the other buffalo records in the GenBank. Seventeen transcription factor binding sites (TFBSs) span along the sequence were predicted.
{"title":"MOLECULAR CHARACTERIZATION OF CYTOCHROME P450 AROMATASE (CYP19) GENE IN EGYPTIAN RIVER BUFFALOES","authors":"M. Aboelenin, K. Mahrous, Amira El-Kerady, M. Rashed","doi":"10.21608/EJGC.2018.9205","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9205","url":null,"abstract":"Cytochrome P450 aromatase (CYP19) gene catalyzes the last step in the steroidogenesis which convert the androgens to estrogens. Therefore, the objective of this study was to investigate the polymorphism in CYP19 gene and its potential effects in female buffaloes fertility. The DNA was extracted from the blood samples of 81 Egyptian river buffalo females and a 419 bp fragment contained a part of CYP19- distal promoter P1.1 was amplified by PCR which subsequently treated with PvuII restriction enzyme. The PCR-RFLP pattern showed that all the animals had a fixed GG genotype and A allele was not detected. Sequencing of the amplified fragment (GenBank accession No. MF490278 and MF490279) followed by sequence alignment with the GenBank database revealed that the homology among the Egyptian river buffalo target sequence and its orthologues sequences in cattle, sheep and goat was 97, 95 and 93%, respectively. G to A transition SNP (G197A) was detected among individuals representing the Egyptian river buffalo by sequencing. G allele was detected only in the Egyptian buffalo and not in the other buffalo records in the GenBank. Seventeen transcription factor binding sites (TFBSs) span along the sequence were predicted.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49167617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Ali, M. Rashed, A. Abdel-Azeem, T. N. El-Din, E. Metry
Genetic transformation is considered as one of the most favorable options for improvement of crop traits. In this study the regeneration frequency and transformation system were established on the Egyptian sweet potato (Ipomoea batatas (L.) Lam.) cv. Abees and Mabruka. The effect of different hormone combinations and type of explant on shoot regeneration was evaluated. The regeneration percentages from Abees and Mabruka cv. 26.3 and 13.3%, respectively were obtained on Murashige and Skoog MS basal salt mixture + 1.0 mg/l BA + 30.0 g/l sucrose + 2.2 g/l Phytagel with Abees cv. and the same media was used for cv. Mabruka with only cytokinin type different as 5.0 Kin and shoots were rooted on MS medium + 30 g/l sucrose and 2.2 g/l Phytagel. The Agrobacterium-mediated and microprojectile bombardement transformation system were successfully introducing the reporter gus and selectable bar marker genes in the sweet potato explants under pressure of 900 and 1100 psi and microcarrier travel distance (6 and 9 cm). Incorporation and expression of the gus and bar genes into sweet potato plants were confirmed using polymerase chain reaction (PCR) and GUS histochemical assay. Several factors were found to be important for regeneration and transformation in sweet potato. The most effective factors were plant genotype and the type of explants. Co-cultivation time and optical density of the Agrobacterium suspension were also critical for sweet potato transformation. This work is an attempt to open the door for further genetic improvement of sweet potato using important agronomic traits.
{"title":"REGENERATION AND TRANSFORMATION SYSTEM IN EGYPTIAN SWEET POTATO (Ipomoea batatas Lam.) CULTIVARS","authors":"N. Ali, M. Rashed, A. Abdel-Azeem, T. N. El-Din, E. Metry","doi":"10.21608/EJGC.2018.9207","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9207","url":null,"abstract":"Genetic transformation is considered as one of the most favorable options for improvement of crop traits. In this study the regeneration frequency and transformation system were established on the Egyptian sweet potato (Ipomoea batatas (L.) Lam.) cv. Abees and Mabruka. The effect of different hormone combinations and type of explant on shoot regeneration was evaluated. The regeneration percentages from Abees and Mabruka cv. 26.3 and 13.3%, respectively were obtained on Murashige and Skoog MS basal salt mixture + 1.0 mg/l BA + 30.0 g/l sucrose + 2.2 g/l Phytagel with Abees cv. and the same media was used for cv. Mabruka with only cytokinin type different as 5.0 Kin and shoots were rooted on MS medium + 30 g/l sucrose and 2.2 g/l Phytagel. The Agrobacterium-mediated and microprojectile bombardement transformation system were successfully introducing the reporter gus and selectable bar marker genes in the sweet potato explants under pressure of 900 and 1100 psi and microcarrier travel distance (6 and 9 cm). Incorporation and expression of the gus and bar genes into sweet potato plants were confirmed using polymerase chain reaction (PCR) and GUS histochemical assay. Several factors were found to be important for regeneration and transformation in sweet potato. The most effective factors were plant genotype and the type of explants. Co-cultivation time and optical density of the Agrobacterium suspension were also critical for sweet potato transformation. This work is an attempt to open the door for further genetic improvement of sweet potato using important agronomic traits.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47664721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abo-Doma, Marwa Mahmoud Shehata, Nahla El-Sherif, M. Ammar, K. Khar
This work aimed to assess the biodiversity of eighteen almond genotypes grown in Libya on the bases of some agronomical, biochemical and molecular characteristics. The agronomical traits were: fruit length, fruit diameter, fruit fresh and dry weights, number of fruits/tree and total yield/tree. The biochemical traits were: total oil, linoleic acid and protein contents. In this context, it was very important to establish an accurate DNA fingerprints for cultivar characterization in order to identify the genetic diversity among these genotypes. For this purpose, 20 combinations of SRAP and 9 primers of ISSR molecular markers were applied to discriminate the 18 almond (Prunus Amygdalus, dulcis L.) genotypes. SRAP generated polymorphic and unique bands for all genotypes. SRAP generated 98 bands, out of these, 22 were common bands while 14 were unique ones used as molecular markers, eight bands out of these unique bands could be considered as positive markers and six as negative markers. ISSR generated 111 bands, out of which, there were three common bands and 19 unique ones, fourteen bands out of them could be considered as positive markers and five as negative markers for a particular genotype. Similarity indices generated shuffling in the arrangement in the cultivar relations according to the two used molecular techniques (SRAP and ISSR), as in SRAP, the two most closely related cultivars were Ansperabilla and Faccinado with similarity value of 0.819, while it was 0.683 between genotypes Ansperabilla and Faccinado as revealed from ISSR data. On the other hand, the two most distantly related cultivars, according to SRAP data, were Avola and Castilla, with similarity value of 0.423, while it was 0.184 between City Bianca and Castilla genotypes according to ISSR data. Dendrogram was conducted for the eighteen genotypes under investigation using the two used molecular techniques. The results revealed different distances in their genetic relationships among the eighteen almond genotypes under investigation.
{"title":"BIODIVERSITY ASSESSMENT FOR SOME ALMOND GENOTYPES CULTIVATED IN LIBYA USING SRAP AND ISSR","authors":"A. Abo-Doma, Marwa Mahmoud Shehata, Nahla El-Sherif, M. Ammar, K. Khar","doi":"10.21608/EJGC.2018.9212","DOIUrl":"https://doi.org/10.21608/EJGC.2018.9212","url":null,"abstract":"This work aimed to assess the biodiversity of eighteen almond genotypes grown in Libya on the bases of some agronomical, biochemical and molecular characteristics. The agronomical traits were: fruit length, fruit diameter, fruit fresh and dry weights, number of fruits/tree and total yield/tree. The biochemical traits were: total oil, linoleic acid and protein contents. In this context, it was very important to establish an accurate DNA fingerprints for cultivar characterization in order to identify the genetic diversity among these genotypes. For this purpose, 20 combinations of SRAP and 9 primers of ISSR molecular markers were applied to discriminate the 18 almond (Prunus Amygdalus, dulcis L.) genotypes. SRAP generated polymorphic and unique bands for all genotypes. SRAP generated 98 bands, out of these, 22 were common bands while 14 were unique ones used as molecular markers, eight bands out of these unique bands could be considered as positive markers and six as negative markers. ISSR generated 111 bands, out of which, there were three common bands and 19 unique ones, fourteen bands out of them could be considered as positive markers and five as negative markers for a particular genotype. Similarity indices generated shuffling in the arrangement in the cultivar relations according to the two used molecular techniques (SRAP and ISSR), as in SRAP, the two most closely related cultivars were Ansperabilla and Faccinado with similarity value of 0.819, while it was 0.683 between genotypes Ansperabilla and Faccinado as revealed from ISSR data. On the other hand, the two most distantly related cultivars, according to SRAP data, were Avola and Castilla, with similarity value of 0.423, while it was 0.184 between City Bianca and Castilla genotypes according to ISSR data. Dendrogram was conducted for the eighteen genotypes under investigation using the two used molecular techniques. The results revealed different distances in their genetic relationships among the eighteen almond genotypes under investigation.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47399031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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