{"title":"DEVELOPMENT OF LATEX AGGLUTINATION TEST FOR RAPID DETECTION OF CITRUS TRISTEZA CLOSTEROVIRUS","authors":"K. Abdalla, H. N. El-Din","doi":"10.21608/EJGC.2013.9959","DOIUrl":null,"url":null,"abstract":"Reliable, simple, and rapid detection tests are needed to better monitor and manage plant virus infection. Microsphere-based diagnostic tests can fulfill these needs and are inexpensive and portable for any laboratory. In the present study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting Citrus tristeza virus (CTV) was developed. Polyclonal antibodies were immobilized on the surface of latex beads and tested for the ability to detect CTV. Under optimized conditions, LAT revealed successful detection of the viral particles in the infected citrus plant.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"42 1","pages":"223-231"},"PeriodicalIF":0.0000,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Genetics and Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EJGC.2013.9959","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Reliable, simple, and rapid detection tests are needed to better monitor and manage plant virus infection. Microsphere-based diagnostic tests can fulfill these needs and are inexpensive and portable for any laboratory. In the present study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting Citrus tristeza virus (CTV) was developed. Polyclonal antibodies were immobilized on the surface of latex beads and tested for the ability to detect CTV. Under optimized conditions, LAT revealed successful detection of the viral particles in the infected citrus plant.
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