GENETIC ANALYSIS IN SOME Cucurbitaceae PLANTS

Abstract

The present study was carried out to analysis the genetic differentiation between seven species of Cucurbitaceae by using biochemical and molecular markers. Analysis of SDS-PAGE of soluble proteins for the seven Cucurbitaceae species such as: Cucumis melo L., Citrullus lanatus L., Citrullus sativtus L., Citrullus colocynthis L., Cucumis prophetarum, Cucurbita pepo L. and Corallocarpus schempri, revealed polymorphism of 98%. Seven isozyme systems including Acp (acid phosphatase), Adh (alchol dehydrogenase), α- and β-Est (esterases), Ao (Aldehyde oxidase), Mdh (malate dehydrogenase) and Prx (peroxidase) successfully indicated genetic variability estimated as 70% polymorphism discriminating between seven Cucurbitaceae species. Six preselected RAPD primers were used in the present study to discriminating between the seven Cucurbitaceae species. RAPD markers revealed (99%) polymorphism that is considerable the highest polymorphism among seven Cucurbitaceae species and the lowest similarity (1%), ISSR markers revealed (98%) polymorphism and finally, AFLP gave (54.8%) of polymorphism with the lowest polymorphism indicating the highest similarity (46%). Based on the results and polymorphism percentages, RAPD, ISSR, SDS-PAGE of soluble proteins, isozymes and AFLP were useful molecular and biochemical tools to discriminate between the seven Cucurbitaceae species.