DETERMINATION OF THE RELATIONSHIPS BETWEEN Pasteurella multocida ISOLATED FROM DIFFERENT FARM ANIMALS AND THEIR HOST RANGE CONTACTS IN EGYPT USING BIOCHEMICAL AND MOLECULAR TECHNIQUES

Abstract

The aim of the present study was to identify and compare the variations among clinical of P. multocida isolates originating from different hosts, sheep (n = 4), buffalo (n = 4) and cattle (n = 4). These isolates were obtained from various locations in the Fayoum governorate, Egypt and they were analyzed by morphological, API kit and RAPD PCR at molecular level to check the distribution of Pneumonia and hemorrhagic septicemia in different farm animals. The chosen isolates exhibited luxuriant growth on blood agar with translucent grayish or yellowish green colonies. However, they showed no growth on MacConkey and no hemolysis of blood; also isolates were positive for oxidase, catalase and H2S production, negative of urease and can ferment glucose, fructose and glucose but can't ferment lactose. Antibiotic sensitivity showed that isolates were sensitive to tetracycline, clindamycin and erythromycin but resistant to ampicillin, trimethoprim/sulfamethoxazole and flucloxacillin. To determine the genetic diversity nine random primers OPA-01  OPA09 were used to which generate different profiles. The distinct RAPD profiles of p. multocida strains showed specificity with regard to the host and the disease induced.