DETECTION OF SOMACLONAL VARIATIONS IN TOMATO USING RAPD MARKERS

Egyptian Journal of Genetics and Cytology Pub Date : 2018-01-01 DOI:10.21608/EJGC.2018.9505

A. Ali, M. E. El-denary, A. El‐Gendy, O. Galal, M. Ahmad, Tahany R. El-Sayed

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引用次数: 6

Abstract

Eleven random primers were used to study somaclonal variation among sixteen somaclones derived from four parental genotypes (Super Strain B, Castle Rock, Advanttage II and Edkawy). Out of eleven random primers used, only six primers (OPA01, OPA02, OPA03, OPA04, OPA05 and OPB11) successfully produced scoreable RAPD bands for all the tested genotypes. DNA amplification with the six different primers generated 285 bands 130 of them were polymorphic and 155 monomorphic bands in all the genotypes studied. Among the primers used, OPA05 produced the highest number of bands (16 bands) while primers OPA04 produced the lowest number (12 bands). RAPD patterns generated by these primers achieved high polymorphic percentage, indicating high level of genetic variations among somaclones and their parental genotypes. Super Strain B cultivar showed the highest number of polymorphic percentage 54.8%, while Advanttage II and Edkawy cultivars showed the lowest percentage of somaclonal variations 42.3% and 42.2%, respectively. The relatively high frequency of variations detected here might bias stable plant propagation. However it could be regarded as a novel source of tomato improvement.

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利用快速标记检测番茄体细胞无性系变异

利用11条随机引物对来自4个亲本基因型(Super Strain B、Castle Rock、advantage II和Edkawy)的16个体细胞无性系进行变异研究。在所使用的11个随机引物中，只有6个引物(OPA01、OPA02、OPA03、OPA04、OPA05和OPB11)成功地对所有测试的基因型产生可评分的RAPD条带。6种引物扩增得到285条条带，其中多态性条带130条，单态条带155条。在所使用的引物中，引物OPA05产生的条带数最多(16条)，引物OPA04产生的条带数最少(12条)。这些引物生成的RAPD模式具有较高的多态性百分比，表明体细胞无性系及其亲本基因型之间存在较高的遗传变异。超级品系B品种的体细胞无性系变异率最高，为54.8%，优势品系II和Edkawy品种的体细胞无性系变异率最低，分别为42.3%和42.2%。这里检测到的相对高频率的变异可能会影响植物的稳定繁殖。然而，它可以被视为番茄改良的新来源。

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Egyptian Journal of Genetics and Cytology

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12 weeks