EPIDEMOLOGI MOLEKULER GENOTIPE Human Immunodeficiency Virus -1 (HIV-l) PADA ORANG DENGAN HIV/AIDS (Acquired Immunodeficiency Syndrome) atau ODHA di JAWA TIMUR DAN DKI JAKARTA

Abstract

Human Immunodeficiency Virus is retrovirus that can decrease immune system in human body. There are two types of HIV which could be genetically specified as HIV-I and HIV-2. HIV-I consists of three groups: M, N, and 0. The 'M' group is further classified as nine subtypes i. e, A, B, C, D, E, F, G, H, and I subtypes. In certain condition, two viruses from different subtypes can mix and form a hybrid virus, called Circulating Recombinant Forms (CRFs). The aim of this research is to map the distribution of HIV subtypes in all of Indonesia's provinces. Sera from HIV patients from hospitals in DKI Jakarta and East Java were taken and examined for genetic analysis. As we may already aware that DKI Jakarta and East Java are provinces that have high prevalence of HIV. All of the specimens were tested and analyzed using RT-PCR technique followed by PCR Nested and sequences confirmation. In East Java Province, HIV-I AE subtypes was the most dominant with 74%followed by Band E subtypes, each of them I3%. In DKI Jakarta province, HIV-I E subtype was the most dominant 60%, AE and B subtypes were 35%, and 5% respectively