Assessment of genotoxic effects of a fungicide product and its active substances on human peripheral blood mononuclear cells

Hayal Cobanoglu, B. Çoşkun, A. Çayır
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引用次数: 1

Abstract

The genotoxic potential of the plant protection product Signum? and its two active substances (pyraclostrobin and boscalid) was investigated by the comet assay (pH>13). Leukocytes isolated from whole blood were treated with different concentrations (0.1-25 ?g/ml) of the fungicide for 2 h and 20 h. The Trypan Blue exclusion test showed higher cell viability (>84%) under all three concentrations and in the two treatment periods. The obtained results revealed that both Signum? and pyraclostrobin induced statistically significant DNA damage. In contrast, boscalid did not cause statistically significant DNA damage after 2 h exposure although it caused DNA damage at higher concentrations after a longer time exposure (20 h). It is deducible that pyraclostrobin and Signum? might be genotoxic. However, within the studied concentration ranges, none of the fungicides was found to be cytotoxic in the two treatment periods.
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杀菌剂产品及其活性物质对人外周血单个核细胞的遗传毒性评价
植物保护产品Signum的基因毒性潜力?用彗星法(pH值bbbb13)对其活性物质pyraclostrobin和boscalid进行了研究。用不同浓度(0.1 ~ 25 μ g/ml)的杀菌剂处理全血白细胞2 h和20 h,台锥蓝排斥试验显示,在这3种浓度和2个处理时期,细胞活力均较高(>84%)。所得结果显示,Signum?pyraclostrobin引起的DNA损伤有统计学意义。相比之下,boscalid在暴露2 h后没有造成统计学意义上的DNA损伤,尽管在暴露较长时间(20 h)后,boscalid在较高浓度下会造成DNA损伤。可能是基因毒性的。然而,在研究的浓度范围内,在两个处理期间,没有发现任何杀菌剂具有细胞毒性。
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审稿时长
4 weeks
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