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Can Sclerotinia stem and root rot be managed effectively without causing environmental imbalance in soil? 能否在不造成土壤环境失衡的情况下有效治理菌核病茎、根腐病?
Pub Date : 2023-01-01 DOI: 10.2298/pif2301011m
M. Mihajlović, E. Rekanović, J. Hrustić, M. Grahovac, Marija Stevanović, B. Tanović
Sclerotinia stem and root rot, caused by Sclerotinia sclerotiorum, is considered to be an important soil-borne disease of over 400 plant species, including a wide range of species important for agriculture. In vitro and in vivo sensitivity of S. sclerotiorum to several commercial fungicides and biofungicides was studied. The highest efficacy was achieved by boscalid (98%) and fluopyram (80%), and the lowest by a B. subtilis-based product (5%). The isolate was sensitive to all tested products in vitro. Considering the tested synthetic fungicides, fluopyram exhibited the highest toxicity (EC50=0.003 mg/l), while captan exhibited the lowest (EC50=8.94mg/l). Even lower efficacy was achieved by tea tree oil and B. subtilis-based products. The environmental impact of pesticides and biopesticides used for Sclerotinia control was assessed. Modeling of predicted environmental concentrations in soil (PECsoil), coupled with literature toxicity data, served for assessment of pesticides soil risks. A high long-term risk for earthworms was revealed for captan and thiophanate-methyl. Based on both efficacy and risk assessment results, fluopyram was found to have the best properties of all tested conventional pesticides, while tea tree oil exerted better performance than the Bacillus product. Further investigation of combined use of conventional and biopesticides might reveal new perspectives regarding effective Sclerotinia control, while simultaneously reducing negative environmental impact.
菌核病是由菌核菌(Sclerotinia sclerotiorum)引起的一种重要的土传病害,影响了400多种植物,其中包括广泛的农业重要物种。研究了菌核菌对几种市售杀菌剂和生物杀菌剂的体内外敏感性。boscalid(98%)和fluopyram(80%)的效果最高,枯草芽孢杆菌产品的效果最低(5%)。该分离物对所有体外试验产品均敏感。在所测试的合成杀菌剂中,氟吡喃的毒性最高(EC50=0.003 mg/l),而队长的毒性最低(EC50=8.94mg/l)。茶树精油和枯草芽孢杆菌产品的效果更低。评价了用于防治菌核病的农药和生物农药对环境的影响。预测土壤环境浓度模型(PECsoil),结合文献毒性数据,用于农药土壤风险评估。研究显示,船长和甲基硫代盐对蚯蚓有很高的长期危害。从功效和风险评价结果来看,氟吡喃在所有被试的常规农药中性能最好,而茶树油的性能优于芽孢杆菌产品。对常规农药和生物农药联合使用的进一步研究可能为有效防治菌核菌提供新的视角,同时减少对环境的负面影响。
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引用次数: 0
Sensitivity of Cuscuta species and their hosts to Anethum graveolens essential oil 菟丝子及其寄主对茴香精油的敏感性
Pub Date : 2023-01-01 DOI: 10.2298/pif2301033s
M. Sarić-Krsmanović, J. Gajić-Umiljendić, L. Radivojević, L. Santric, T. Djordjevic, Rada Djurovic-Pejcev
The purpose of this study was to examine in vitro the herbicidal effect of an essential oil of dill (Anethum graveolens) on germination and early seedling growth of two parasitic flowering plants in the genus Cuscuta (Cuscuta campestris and C. epithymum), as well as its phytotoxic impact on germination and early seedling growth of two host plants (alfalfa and red clover). Chemical analysis of the essential oil extracted from dill leaves and flowers showed that carvone (51.69%) and limonene (39.88%) predominated. The results of a seed bioassay showed inhibitory effects of different concentrations (1%, 0.5%, 0.1%, and 0.01% v v-1) of the essential oil of dill leaves on germination and early seedling growth of both tested species of the genus Cuscuta. Germination of C. campestris seeds was inhibited between 67% and 94%, while seed germination of C. epithymum was inhibited between 67% and 100%. A similar inhibitory effect was observed regarding the seedling length parameter. Moreover, the bioassay results indicated significant phytotoxic effects of dill essential oil on the seed germination and early seedling growth of host plants. Red clover proved more sensitive as even the lowest concentration inhibited germination by 35%, while higher concentrations caused 70-100% inhibition. On the other hand, lower concentations inhibited germination of alfalfa seeds by 5%, while higher concentrations caused greater inhibition (36-100%). Future research should include both pot experiments and field micro-trials to determine herbicidal, i.e. phytotoxic, effects of dill essential oil on parasitic flowering plants and cultivated species.
本研究旨在体外研究莳萝精油对菟丝子属两种寄生开花植物(Cuscuta campestris和C.上皮植物)萌发和早苗生长的除草作用,以及对两种寄主植物(苜蓿和红三叶草)萌发和早苗生长的植物毒性影响。从莳萝叶和花中提取的精油化学成分分析表明,香芹酮(51.69%)和柠檬烯(39.88%)含量最高。种子生物测定结果表明,不同浓度(1%、0.5%、0.1%和0.01% v-1)的莳萝叶精油对两种菟丝子属植物的萌发和幼苗生长均有抑制作用。对油菜种子萌发的抑制率为67% ~ 94%,对芽孢杆菌上皮细胞种子萌发的抑制率为67% ~ 100%。在幼苗长度参数上也观察到类似的抑制效应。此外,生物测定结果表明,莳萝精油对寄主植物的种子萌发和幼苗早期生长有显著的植物毒性作用。红三叶草对草籽萌发的抑制作用较强,最低浓度的草籽萌发抑制率为35%,较高浓度的草籽萌发抑制率为70-100%。另一方面,低浓度对苜蓿种子萌发的抑制作用为5%,而高浓度对苜蓿种子萌发的抑制作用更大(36-100%)。未来的研究应包括盆栽试验和田间微试验,以确定莳萝精油对寄生开花植物和栽培物种的除草作用,即植物毒性作用。
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引用次数: 0
Comparative toxicity of spinetoram to Trialeurodes vaporariorum Westwood and its parasitoid Eencarsia formosa Gahan 刺螨对白刺螟及其寄生蜂台湾飞蛾的毒性比较
Pub Date : 2023-01-01 DOI: 10.2298/pif2302065d
Tanja Drobnjakovic, Mirjana Prijovic, Emanuele Porcu, Michele Ricupero, Gaetano Siscaro, Lucia Zappala, Antonio Biondi
The role of selective new generation bioisecticides, beside their effectiveness against key pests, relies on their safety to beneficial arthropods. Spinetoram, a semi-synthetic analogue of the microbial-derived bioinsecticide spinosad is registered worldwide for application in numerous crops, but assessment of its ecotoxicological risk to beneficial arthropods has scarcely been documented. Moreover, this is the first report on toxic effects of spinetoram on a pest, the greenhouse whitefly Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), and/or its successful biocontrol agent, the parasitoid Encarsia formosa Gahan (Hymenoptera: Aphelinidae). Under laboratory conditions, we assessed the acute toxicity of spinetoram insecticide (25% a.i.) to adults, nymphs and eggs of the greenhouse whitefly, as well as to parasitoid adults and pupae. In all concentration-response bioassays, the spinetoram insecticide was applied to tobacco leaves settled onto 1% agar layer in ventilated Petri dishes using a Potter spray tower. The parameters of spinetoram acute toxicity to adults of both the pest and the parasitoid were evaluated in residual contact bioassays, while whitefly eggs and nymphs, and parasitoid pupae were topically treated with a series of spinetoram concentrations, covering a range of 10-90% mortality. Lethal spinetoram effects on the parasitoid E. formosa were assessed through selectivity ratio (SR) estimations, showing the ratios beetween median lethal concentrations (LC50s) estimated for the parasitoid, and LC50s estimated for the pest. The following LC50 values were obtained: 4.593, 15.027 and 11.73 mg a.i./l for whitefly adults, nymphs and eggs, respectively, and 0.686 and 1.715 mg a.i./l for parasitoid adults and pupae, respectively. The calculated SR estimations were below 1, indicating that spinetoram insecticide is non-selective to both tested stages of the parasitoid E. formosa. A more detailed understanding of spinetoram impact on E. formosa in whitefly integrated management requires further evaluation of sublethal effects and greenhouse trials, with an emphasis on population-level responses.
选择性新一代生物杀虫剂的作用,除了对主要害虫有效外,还取决于它们对有益节肢动物的安全性。Spinetoram是一种微生物衍生生物杀虫剂spinosad的半合成类似物,已在全球范围内注册用于许多作物,但其对有益节肢动物的生态毒理学风险评估几乎没有记录。此外,这是第一次报道spinetoram对温室白蛉(半翅目:白蛉科)和/或其成功的生物防治剂——寄生蜂(膜翅目:白蛉科)的毒性作用。在实验室条件下,测定了25%杀虫剂spinetoram对温室白蝇成虫、若虫和卵以及寄生蜂成虫和蛹的急性毒性。在所有浓度响应生物试验中,使用波特喷雾塔将spinetoram杀虫剂施用于烟叶,烟叶沉降在通风培养皿中1%的琼脂层上。采用残留接触生物测定法对白蝇和拟寄生虫成虫的急性毒性参数进行了评价,并对白蝇卵、若虫和拟寄生虫蛹进行了不同浓度的刺虫虫局部处理,死亡率在10 ~ 90%之间。通过选择性比(SR)估计,评估了刺曲仑对台湾寄生蜂的致死效应,显示了寄生蜂估计的中位致死浓度(lc50)与害虫估计的lc50之间的比率。结果表明:粉虱成虫、若虫和卵的LC50分别为4.593、15.027和11.73 mg a.i./l,寄生蜂成虫和蛹的LC50分别为0.686和1.715 mg a.i./l。计算得到的SR值均小于1,表明该杀虫剂对台湾寄生蜂的两个试验阶段均无选择性。为了更详细地了解白蝇综合管理中spinetoram对台湾白蝇的影响,需要进一步评估亚致死效应和温室试验,重点是种群水平的反应。
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引用次数: 0
Antioxidant activity, phytotoxicity and allelopathic potential of green walnut (Juglans regia L.) fruit extract 青核桃果实提取物的抗氧化活性、植物毒性和化感作用
Pub Date : 2023-01-01 DOI: 10.2298/pif2302075s
Marija Saric-Krsmanovic, Jelena Gajic-Umiljendic, Tijana Djordjevic, Ljiljana Radivojevic, Ljiljana Santric, Dragana Bozic, Sava Vrbnicanin
The potential allelopathic effect of a green walnut fruit extract on seed germination and early growth of three weed species (Chenopodium album, Amaranthus retroflexus, Daucus carota) was tested, as well as its phytotoxic effect on seed germination and early growth of maize (Zea mays). Another objective was to analyze the plant extract and assess its antioxidative activity. Antioxidative activity of the plant extract was evaluated based on its ability to neutralize DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and capacity for iron reduction using the FRAP method. Inhibition of the evaluated parameters (total germination and seedling length) decreased proportionally with decreasing concentrations of extract solution of green walnut fruit. The parameters of seedling growth were also found to show greater susceptibility than total seed germination of all three of the tested weed species. Although the seed bioassay results indicated a high inhibitory effect on germination and early growth of the tested weeds, they also revealed phytotoxic activity during early growth of the cultivated Z. mays.
研究了青核桃果提取物对三种杂草(Chenopodium album, Amaranthus retroflexus, Daucus carota)种子萌发和早期生长的潜在化感作用,以及对玉米(Zea mays)种子萌发和早期生长的植物毒性作用。另一个目的是分析植物提取物并评估其抗氧化活性。采用FRAP法对植物提取物的抗氧化活性进行了评价,评价了其对DPPH(2,2-二苯基-1-苦味酰肼)自由基的中和能力和对铁的还原能力。青核桃果浸膏溶液浓度越低,对总发芽率和幼苗长度的抑制率越低。幼苗生长参数对三种杂草的敏感性均高于总种子萌发率。虽然种子生物测定结果显示对被试杂草的萌发和早期生长有很高的抑制作用,但它们也显示出在培养的麦穗草的早期生长中具有植物毒性活性。
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引用次数: 0
Influence of Xanthomonas euvesicatoria on quality parameters of pepper seed from Serbia 辣椒黄单胞菌对塞尔维亚辣椒种子品质参数的影响
Pub Date : 2023-01-01 DOI: 10.2298/pif2301001z
Ivana Živković, R. Iličić, G. Barać, J. Damnjanovic, D. Cvikic, N. Trkulja, Tatjana Popovic-Milovanovic
The present study focused on detecting bacteria of the Xanthomonas spp. complex (X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri) and examining their influence on certain quality parameters of pepper seed collected from the territory of Smederevska Palanka (Serbia). The analysis included 27 non-commercial pepper seed genotypes (including chili and sweet pepper) collected in 2021. Several parameters of the quality of analyzed pepper seed (germination energy, total germination, moisture and seed health) were determined. The results showed that out of a total of 27 analyzed samples of pepper seed, the presence of X. euvesicatoria was detected in 13 of them. The presence of X. vesicatoria, X. gardneri and X. perforans was not confirmed. Germination energy of infected seed was 52-84%, and of bacteria-free seed 63-90%; total germination of infected seed was 66-91%, and of bacteria-free seed 80-95%. Seed moisture of infected seed samples was 6.1-12%, and of bacteria-free seed 6.2-8.1%. These parameters did not show significant statistical difference (p>0.05). The presence of seed-borne fungi Fusarium sp. accounted for up to 3% in 25 samples, while it was up to 6% in the remaining two; Alternaria sp. ranked from 1-4% in 25 samples, and up to 5% in only two samples. The results led to a conclusion that the bacterium X. euvesicatoria is the predominant pathogen of the Xanthomonas spp. complex, but it did not affect the quality parameters of the tested pepper seed.
本研究对采自塞尔维亚斯梅代夫斯卡帕兰卡地区辣椒种子的黄单胞菌(Xanthomonas spp. complex) (X. euvesicatoria、X. vesicatoria、X. perforans和X. gardneri)进行了细菌检测,并考察了它们对辣椒种子某些品质参数的影响。该分析包括2021年收集的27种非商业辣椒种子基因型(包括辣椒和甜椒)。测定了所分析辣椒种子的发芽能、总发芽率、水分和种子健康等质量参数。结果表明,在27份辣椒种子样品中,有13份样品中检出了绿僵菌。没有确定是否存在膀胱x线虫、加德纳x线虫和穿孔x线虫。感染种子萌发率为52 ~ 84%,无菌种子萌发率为63 ~ 90%;感染种子总发芽率为66-91%,无菌种子总发芽率为80-95%。感染种子样品的水分为6.1 ~ 12%,无菌种子样品的水分为6.2 ~ 8.1%。两组间差异无统计学意义(p < 0.05)。25份样品中镰刀菌的存在率高达3%,其余2份样品中镰刀菌的存在率高达6%;在25个样本中,交替孢属的排名在1-4%之间,只有两个样本的排名高达5%。结果表明,辣椒黄单胞菌复合体的主要病原菌为葡萄球菌,但对辣椒种子的质量参数没有影响。
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引用次数: 0
Stemphylium vesicarium (wallr.) E.G. Simmons: An onion plant pathogen and options for suppression 水仙茎(壁)一种洋葱植物病原体及其抑制方法
Pub Date : 2023-01-01 DOI: 10.2298/pif2302043t
Ana Takac, Slavica Vukovic
Onion (Allium cepa L.) is one of the most important vegetable species grown worldwide, including the Republic of Serbia. Leaf blight, caused by the fungus Stemphylium vesicarium, is a serious and destructive disease of onion leaves around the world, which limits the quality and quantity of bulbs and seeds. Yield decrease occurs due to a reduced photosynthetic area, which leads to the formation of smaller bulbs of poorer quality. The recommended strategy for control and reduction of SLB inoculum includes crop rotation with other vegetable species or cereals that are not hosts of these fungi, the use of resistant onion genotypes, weed removal, adequate use of nitrogen fertilizers, control of thrips (Thrips spp.), as well as seed treatment, considering that seeds play a significant role in the spread of pathogens. Timely and correct application of foliar fungicides is certainly the key strategy. The timing of application of fungicides with different modes of action is crucial for controlling Stemphylium vesicarium in onion.
洋葱(Allium cepa L.)是世界上最重要的蔬菜品种之一,包括塞尔维亚共和国。洋葱叶枯病是由洋葱茎枯菌(Stemphylium vesicarium)引起的一种严重的破坏性洋葱叶病,严重影响洋葱鳞茎和种子的质量和数量。由于光合作用面积减少,产量下降,导致球茎变小,质量变差。控制和减少SLB接种量的建议策略包括与不是这些真菌宿主的其他蔬菜或谷物轮作,使用抗性洋葱基因型,除草,充分使用氮肥,控制thrips (thrips spp.),以及考虑到种子在病原体传播中起重要作用的种子处理。及时、正确地施用叶面杀菌剂当然是关键策略。不同作用方式的杀菌剂的施用时机是防治洋葱茎枯病的关键。
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引用次数: 0
Corrigendum to “The dark-red spider mite, Tetranychus ludeni Zacher (Acari: Tetranychidae) - a new pest in Serbian acarofauna” Ivana Maric, Irena Medjo and Dejan Marcic (volume 37, issue 3, pp. 85-93; doi: https://doi.org/10.2298/PIF2203085M) Ivana Maric, Irena Medjo和Dejan Marcic(第37卷,第3期,第85-93页)对“深红色蜘蛛螨,Tetranychus ludeni Zacher(螨亚纲:叶螨科)-一种新的塞尔维亚螨纲害虫”的更正;doi: https://doi.org/10.2298/PIF2203085M)
Pub Date : 2023-01-01 DOI: 10.2298/pif2301041e
E. Editorial
The original published version of the article contained two errors: (1) In Table 1B: tomato was not indicated as a host plant for T. evansi (2) In Figure 1: After carefully looking at the previous drawings of T. ludeni body parts, we concluded that it is necessary to provide new and more precise drawings of the male aedeagus and female tibia/tarsus I The corrigendum presents the corrected version of Table 1B and Figure 1.

Link to the corrected article 10.2298/PIF2203085M
这篇文章最初发表的版本有两个错误:(1)在表1B中:番茄没有被指出是T. evansi的寄主植物(2)在图1中:在仔细查看了T. ludeni身体部位的先前图纸后,我们得出结论,有必要提供新的和更精确的雄性aedeagus和雌性胫骨/跗骨的图纸。链接到更正后的文章10.2298/PIF2203085M
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引用次数: 0
Management of ginger bacterial wilt (Ralstonia solanacearum) epidemics by soil solarization and botanical mulching at Tepi, Southwestern Ethiopia 埃塞俄比亚西南部Tepi地区土壤日晒和植物覆盖对生姜青枯病(Ralstonia solanacearum)流行的治理
Pub Date : 2023-01-01 DOI: 10.2298/pif2301023j
Merga Jibat, Abukiya Getu
Ginger is one of the most valuable cash crops for farmers in different parts of Ethiopia. Bacterial wilt disease, caused by Ralstonia solanacearum, creates major limitation to production of ginger in Ethiopia. Heavy losses due to the disease occur regularly, causing an additional impediment for production in infected areas. Field tests were conducted at Tepi, South- Western Ethiopia, during the 2019 and 2020 main cropping seasons to assess the effects of soil solarization and botanical mulch on epidemics of bacterial wilt of ginger. Four soil solarization periods, lasting two, four, six or eight weeks before planting were integrated with four different botanical mulch treatments after planting: vetivar grass, lemon grass, Chinese chive and Lantana camara. Unsolarized and unmulched plots were used as the control for comparison. Treatments were arranged in factorial arrangements with randomized complete block design with three replications. The outcomes indicated that soil solarization integrated with lemon grass mulch treatments significantly reduced bacterial wilt mean incidence by 22.1% up to 42.2%, compared to control plots. These treatments also dramatically reduced AUDPC and disease progress rates. Soil solarization for eight weeks integrated with lemon grass mulch resulted in the lowest (42.2%) final mean disease severity and AUDPC (33.8%) in comparison to the control. Typical results of this study indicated that soil solarization integrated with botanical mulch treatments were effective in slowing dawn the epidemics of bacterial wilt and in recovering ginger production and productivity, and they are consequently recommended for application in the study areas along with other crop management schemes.
姜是埃塞俄比亚不同地区农民最有价值的经济作物之一。由青枯病引起的细菌性枯萎病对埃塞俄比亚的生姜生产造成了重大限制。这种疾病造成的严重损失经常发生,对受感染地区的生产造成了额外的障碍。在2019年和2020年主要种植季,在埃塞俄比亚西南部的Tepi进行了田间试验,以评估土壤日晒和植物覆盖对姜青枯病流行的影响。种植前分别进行2周、4周、6周和8周的4个土壤日晒期,种植后进行香根草、柠檬草、韭菜和山楂四种不同的植物覆膜处理。以未晒地和未覆盖地为对照。处理按因子排列,采用随机完全区组设计,3个重复。结果表明,与对照区相比,土壤光照加柠檬草覆盖可显著降低青枯病平均发病率(22.1% ~ 42.2%)。这些治疗也显著降低了AUDPC和疾病进展率。与对照相比,土壤光照8周并覆盖柠檬草的最终平均疾病严重程度(42.2%)和AUDPC(33.8%)最低。本研究的典型结果表明,土壤日晒与植物覆盖相结合可有效减缓青枯病的流行,恢复生姜的产量和生产力,因此推荐与其他作物管理方案一起在研究地区应用。
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引用次数: 0
Bionomy of Coccus pseudomagnoliarum (Kuwana) (Hemiptera: Coccidae), a new species in the fauna of Serbia 塞尔维亚区系一新种伪magnolia球菌生物学(半翅目:球虫科)
Pub Date : 2023-01-01 DOI: 10.2298/pif2302055d
Marina Dervisevic, Aleksandar Stojanovic, Draga Graora
Citricola scale, Coccus pseudomagnoliarum (Kuwana) (Hemiptera: Coccidae) was for the first time registered in Serbia in 2015 in the area of Belgrade on Celtis occidentalis L. C. pseudomagnoliarum develops one generation annually and overwinters as the second-instar nymph on host twigs. It forms numerous colonies on infested plants, and symptoms of its feeding appear in the form of drying leaves and twigs. In addition, large amounts of honeydew that this scale secretes reduce photosynthesis and transpiration in plants, which accelerates their decay. Citricola scale attracts many entomophagous insects which are able to reduce pest population. The parasitoid wasps Coccophagus lycimnia (Walker), Coccophagus piceae Erdos, Coccophagus scutellaris (Dalman), Coccophagus shillongensis (Hayat and Singh) (Aphelinidae), Cheiloneurus claviger Thomson and Metaphycus stanleyi Compere (Encyrtidae) were reared. C. piceae and M. stanleyi are new species in the fauna of Serbia. C. pseudomagnoliarum is a new host for the species M. stanleyi. The predators Coccinella septempunctata L., Exochomus quadripustulatus (L.) (Coccinellidae) and Chrysoperla carnea (Stephens) (Chrysopidae) were found in scale colonies. The most efficient natural enemy of C. pseudomagnoliarum nymphs was C. lycimnia, reducing scale populations by 11-26%.
2015年,在塞尔维亚贝尔格莱德地区首次发现了一种名为pseudomagnoliarum (Kuwana)(半翅目:球虫科)的瓢虫,寄生于Celtis occidentalis l.c. . pseudomagnoliarum,每年发育一代,在寄主树枝上以二龄若虫的形态越冬。它在受感染的植物上形成大量的菌落,其进食的症状表现为叶子和树枝干燥。此外,这种鳞片分泌的大量蜜露会减少植物的光合作用和蒸腾作用,从而加速植物的腐烂。柑桔鳞片吸引了许多食虫昆虫,可以减少害虫的数量。饲养拟寄生蜂有:lycimnia Coccophagus (Walker), piceae Erdos, scutellaris (Dalman), shillongensis (Hayat and Singh)(蚜虫科),Cheiloneurus claviger Thomson和Metaphycus stanleycompere (encytidae)。C. piceae和M. stanleyi是塞尔维亚区系的新种。C. pseudomagnoliarum是M. stanleyi的新寄主。在鳞片群落中发现捕食者有七星瓢虫(Coccinella七星瓢虫)、四足瓢虫(Exochomus quadripustulatus L.)和瓢虫科(Chrysoperla carnea Stephens)。对假木兰若虫最有效的天敌为lycimnia,可使蚧虫种群减少11% ~ 26%。
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引用次数: 0
Fusarium species associated with wheat head blight disease in Algeria: Characterization and effects of triazole fungicides 阿尔及利亚与小麦头疫病相关的镰刀菌:三唑杀菌剂的特性和效果
Pub Date : 2022-01-01 DOI: 10.2298/pif2202049i
Belabed Imane, Abed Hannane, B. Amor, Rouag Noureddine
Fusarium head blight is an important disease of durum wheat which requires several fungicide treatments of seeds to achieve satisfactory control. The current study was carried out to evaluate commercially available fungicides in vitro for their efficacy against eighteen Fusarium spp. isolates collected from different fields in the north-eastern part of Algeria. The morphological and molecular characterization reveals the presence in wheat seeds of the main species complexes F. acuminatum, F. equiseti, F. avenaceum, F. solani, F. culomorum, F. incarnatum-equiseti, as well as F. tricinctum species complex and F. chlamydosporum species complex. Antifungal activity of fungicides shows that all triazoles tested have proven their effectiveness in inhibiting the mycelial growth of various strains of Fusarium tested. However, their sensitivity varies between them significantly (p<0.05) depending on the dose applied and period of exposure to each fungicide. The results showed that tebuconazole (Raxil and T?buzole) and the combination fludioxonil + difenoconazole greatly reduced the mycelial growth of Fusarium isolates by 84.31%, 82.94%, 81.33%, respectively, as compared to difenoconazole alone (73.16%) at the recommended dose after five days of exposure. Regarding their effect on conidia germination, tebuconazole was more effective than fludioxonil + difenoconazole, which leads to deformation of cell wall structure and fragmentation of conidia. These results will provide useful information to select suitable fungicides for seed treatment and management of wheat head blight disease.
赤霉病是硬粒小麦的重要病害,需要对种子进行多种杀菌剂处理才能达到满意的防治效果。本研究旨在评价市售杀菌剂对阿尔及利亚东北部不同地区18株镰刀菌分离株的体外药效。形态和分子特征表明,小麦种子中主要存在尖锐镰刀菌、马尾镰刀菌、avenaceum镰刀菌、solani镰刀菌、culomorum镰刀菌、incarnatum-equiseti镰刀菌、tricinctum镰刀菌和chlamydosporum镰刀菌复合种。杀菌剂的抑菌活性表明,所测三唑均能有效抑制各种镰刀菌菌丝的生长。然而,它们之间的敏感性差异显著(p<0.05),这取决于所施用的剂量和暴露于每种杀菌剂的时间。结果表明,暴露5 d后,戊唑唑(拉克西和托唑)和氟恶菌腈+二苯醚康唑联用对镰刀菌菌丝生长的抑制作用分别较推荐剂量下的单用(73.16%)显著降低84.31%、82.94%和81.33%。在对分生孢子萌发的影响方面,戊康唑的效果优于氟恶菌腈+异苯康唑,导致细胞壁结构变形,分生孢子碎裂。这些结果将为选择合适的杀菌剂进行小麦穗疫病的种子处理和管理提供有用的信息。
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Pesticidi I Fitomedicina
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