High proportion of mixed virus infections in raspberry plantations in Serbia

S. Paunovic, D. Jevremović
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Abstract

Samples of red raspberry (Rubus idaeus L.) from the most important growing areas in Serbia were analyzed for the presence of four most important raspberry viruses: raspberry leaf blotch virus (RLBV), raspberry leaf mottle virus (RLMV), black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and their incidence in individual and mixed infected samples. Seventy-four samples of eight raspberry cultivars, selected from 32 main cultivation sites were tested, of which 62 leaf samples showed symptoms that could be caused by viruses and 12 samples were asymptomatic. The presence of viruses was tested by appropriate RT-PCR and PCR methods, using virus-specific primers. All four viruses were detected with highly significant differences of incidence in a total of 61 infected symptomatic samples. Infection with one of the four tested viruses was detected in 59.0% of all infected samples. The most prevalent was RLBV, which was associated with raspberry leaf blotch disorder in coexistence with its vector raspberry leaf and bud mite P. gracillis, and it was detected in 70.5% of the infected samples. It was followed by RYNV with an incidence of 42.6%, BRNV with 36.1% and RLMV with 9.8% incidence, considering all infected samples. The obtained results for the first time in Serbia showed a high proportion (41.0%) of mixed infections involving between two and four viruses. The most common were infections with two viruses, 68.0% of all mixed-infected samples, and among them coinfections with RLBV and RYNV (44.0%). Viruses of the raspberry mosaic complex, BRNV, RYNV and RLMV, including coinfections of 2-3 listed viruses along with RLBV, were found in a high joined proportion of 14.7% of all infected samples, i.e. 36.0% of mixed infected samples.
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塞尔维亚覆盆子种植园混合病毒感染比例高
对塞尔维亚主要产区的红覆盆子(Rubus idaeus L.)样品进行了覆盆子叶斑病毒(RLBV)、覆盆子叶斑驳病毒(RLMV)、黑覆盆子坏死病毒(BRNV)和覆盆子黄网病毒(RYNV)四种最重要的覆盆子病毒的检测,并分析了它们在单个和混合感染样品中的发病率。对32个主要栽培地点的8个覆盆子品种74个样品进行了检测,其中62个样品显示可能由病毒引起的症状,12个样品无症状。利用病毒特异性引物,采用适当的RT-PCR和PCR方法检测病毒的存在。在61份有症状的感染样本中检测到所有四种病毒,其发病率存在高度显著差异。在所有感染样本中,59.0%检测到四种检测病毒中的一种感染。其中最常见的是RLBV,其与载体树莓叶芽螨共存,与树莓叶斑病有关,在感染样品中检测到70.5%的RLBV。所有感染样本中,RYNV感染率为42.6%,BRNV为36.1%,RLMV为9.8%。在塞尔维亚首次获得的结果显示,涉及两种和四种病毒的混合感染比例很高(41.0%)。最常见的是两种病毒感染,占所有混合感染样本的68.0%,其中RLBV和RYNV共感染(44.0%)。覆盆子花叶复体BRNV、RYNV和RLMV病毒,包括2-3种列示病毒与RLBV共感染,在所有感染样本中占14.7%,在混合感染样本中占36.0%。
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发文量
4
审稿时长
4 weeks
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