R. Iličić, F. Bagi, M. Blagojević, Jovica Gosic, P. Milovanović, T. Popović
{"title":"Etiology of bacterial diseases of young walnut trees in Serbia","authors":"R. Iličić, F. Bagi, M. Blagojević, Jovica Gosic, P. Milovanović, T. Popović","doi":"10.2298/pif2103101i","DOIUrl":null,"url":null,"abstract":"In the summer and autumn of 2019-2020, young walnut orchards were monitored for the presence of bacterial diseases. Diseased walnut samples comprising trunks and branches with symptoms of vertical oozing canker (VOC), walnut bacterial blight (WBB) and superficial bark necrosis were collected from eight locations in Serbia. Based on phenotypic features, pathogenicity, and molecular assays using PCR with specific primers, 49 isolates obtained from samples showing VOC and WBB symptoms were identified as Xanthomonas arboricola pv. juglandis, while further two isolates obtained from bark necrosis were identified as Brenneria rubrifaciens. One tested X. a. pv. juglandis isolate obtained from a VOC sample produced deep cankers in the bark of inoculated trunks of young walnut trees (cultivars Chandler, Franquette and Sejnovo). Therefore, this is the first report of an association between X. a. pv. juglandis and VOC symptom in Serbia. Considering that X. a. pv. juglandis significantly endangers walnut production, the presence of this pathogen in walnut transplant imports needs to be assessed by an authorised laboratory. Furthermore, as this is also the first report of B. rubrifaciens on walnut trees in Serbia, it is noteworthy that this pathogen is not particularly harmful to young walnut trees.","PeriodicalId":19801,"journal":{"name":"Pesticidi I Fitomedicina","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticidi I Fitomedicina","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/pif2103101i","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
In the summer and autumn of 2019-2020, young walnut orchards were monitored for the presence of bacterial diseases. Diseased walnut samples comprising trunks and branches with symptoms of vertical oozing canker (VOC), walnut bacterial blight (WBB) and superficial bark necrosis were collected from eight locations in Serbia. Based on phenotypic features, pathogenicity, and molecular assays using PCR with specific primers, 49 isolates obtained from samples showing VOC and WBB symptoms were identified as Xanthomonas arboricola pv. juglandis, while further two isolates obtained from bark necrosis were identified as Brenneria rubrifaciens. One tested X. a. pv. juglandis isolate obtained from a VOC sample produced deep cankers in the bark of inoculated trunks of young walnut trees (cultivars Chandler, Franquette and Sejnovo). Therefore, this is the first report of an association between X. a. pv. juglandis and VOC symptom in Serbia. Considering that X. a. pv. juglandis significantly endangers walnut production, the presence of this pathogen in walnut transplant imports needs to be assessed by an authorised laboratory. Furthermore, as this is also the first report of B. rubrifaciens on walnut trees in Serbia, it is noteworthy that this pathogen is not particularly harmful to young walnut trees.
在2019-2020年夏、秋两季对核桃幼园进行了细菌性病害监测。从塞尔维亚的8个地点收集了患病核桃样本,包括树干和树枝,具有垂直渗液溃疡病(VOC)、核桃细菌性枯萎病(WBB)和表面树皮坏死的症状。基于表型特征、致病性和特异引物的PCR分子分析,从VOC和WBB症状的样品中获得的49株分离物被鉴定为树黄单胞菌pv。另外两株从树皮坏死中分离得到的菌株被鉴定为红布氏布伦纳氏菌。一个测试x。a。pv。从挥发性有机化合物样品中获得的juglandis分离物在幼龄核桃树(栽培品种Chandler, Franquette和Sejnovo)接种后的树干树皮上产生深度溃疡病。因此,这是第一个关于X. a. pv。塞尔维亚的腺炎和挥发性有机化合物症状。考虑到x。a。pv。Juglandis严重危害核桃生产,在核桃移植进口中是否存在这种病原体需要由授权实验室进行评估。此外,由于这也是塞尔维亚核桃树上首次报道红分枝杆菌,值得注意的是,该病原体对幼树的危害并不特别大。