Monitoring of pharmaceutical residues of non-steroidal drugs with use of Escherichia coli-gfp biosensors

Abstract

Escherichia coli strains containing a three different plasmid-borne transcriptional fusion between genotoxin-inducible recA, kat G and sodA promoters involved in the SOS regulon and bacteria stress response and mutated form of gfp reporter gene, have been used. GFP-based bacterial biosensors allowed for detection of a cytotoxic and genotoxic activity of ibuprofenum, ketoprofenum and paracetamolum – conventional non-steroidal anti-inflammatory drugs in PBS buffer and surface water. For experimental tests drugs were used at concentration of 10; 10; 10; 10 and 10 mg/dm, with bacteria strains time incubation of 3 and 24 hours. Experimental data indicated, that three promoters fusions with gfp gene as reporter were differently sensitive to applied drugs. Bacteria strains, recA, kat G and sodA promoters were a good bioindicator for cytotoxic and genotoxic effect monitoring of tested drugs in PBS buffer and surface water. The results showed, that applied in this experiment E. coli gfp biosensors strains could be potentially useful for environmental monitoring of cytotoxic and genotoxic effect of pharmacist residues of drugs in surface water.