Assessment of the molecular cytogenetic, morphometric and biochemical parameters of Deschampsia antarctica from its southern range limit in maritime Antarctic

IF 0.9 4区 地球科学 Q4 ECOLOGY Polish Polar Research Pub Date : 2018-01-01 DOI:10.24425/118759
D. Navrotska, Igor Andreev, A. Betekhtin, Magdalena Rojek, I. Parnikoza, G. Myryuta, O. Poronnik, N. Miryuta, Joanna SZYMANOWSKA-PU, Ka, V. Grakhov, R. Ivannikov, R. Hasterok, V. Kunakh
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引用次数: 9

Abstract

Different chromosomal forms of Deschampsia antarctica Desv. (Poaceae), including diploids (2n=26), hypotriploid (2n=36–38) and a genotype with an occasional occurrence of B chromosome (2n=26+0-1B) that originated from southern marginal populations (Argentine Islands region, maritime Antarctic) were studied using molecular cytogenetic, morphometric and biochemical methods. FISH analysis revealed variations in the number of rDNA sites between the diploid and hypotriploid plants. The genome size varied among plants with a different chromosome number and was on average 10.88 pg/2C for diploids and 16.46 pg/2C for hypotriploid. The mean values of leaf length of plants grown in vitro varied within a range of 5.23–9.56 cm. The total phenolic content ranged from 51.10 to 105.40 mg/g, and the total flavonoid content ranged from 1.22 to 4.67 mg/g. The amount of phenolic compounds did not differ significantly between the genotypes, while a variation in the flavonoid content was observed for L59 and DAR12. The diploids did not differ significantly among each other in terms of the number of rDNA loci, but differed slightly in their genome size. The individuals of DAR12 carrying B chromosome were similar to other diploids in terms of their genome size, but Daria Navrotska et al. 526 statistically differed in leaf length. The hypotriploid had both a greater number of rDNA sites and a larger genome size. No statistical correlations were observed between the genome size and leaf length or genome size and accumulation of phenolic and flavonoid compounds. The results of this study suggest that D. antarctica plants from the southern edge of the range are characterised by the heterogeneity of the studied parameters.
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南极海洋南缘南极德尚蚤的分子细胞遗传学、形态计量学和生化参数评价
南极斑蝶的不同染色体形态。采用分子细胞遗传学、形态计量学和生物化学等方法,对阿根廷群岛、南极海域等南部边缘种群中二倍体(2n=26)、半三倍体(2n=36 ~ 38)和一个偶有B染色体的基因型(2n=26+0 ~ 1b)进行了研究。FISH分析显示二倍体和半三倍体植株的rDNA位点数量存在差异。不同染色体数目的植株基因组大小不同,二倍体平均为10.88 pg/2C,半三倍体平均为16.46 pg/2C。离体植株叶长平均值在5.23 ~ 9.56 cm之间。总酚含量为51.10 ~ 105.40 mg/g,总黄酮含量为1.22 ~ 4.67 mg/g。酚类化合物含量在不同基因型间差异不显著,而类黄酮含量在L59和DAR12基因型间存在差异。二倍体在rDNA位点数量上差异不显著,但在基因组大小上差异不大。携带B染色体的DAR12个体在基因组大小上与其他二倍体相似,但Daria Navrotska等526在叶片长度上存在统计学差异。亚三倍体具有更多的rDNA位点和更大的基因组大小。基因组大小与叶片长度、基因组大小与酚类化合物和类黄酮化合物积累均无统计学相关性。本研究结果表明,南缘的南极D. antarctica植物具有研究参数的异质性特征。
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来源期刊
Polish Polar Research
Polish Polar Research ECOLOGY-GEOSCIENCES, MULTIDISCIPLINARY
CiteScore
2.00
自引率
7.70%
发文量
0
审稿时长
>12 weeks
期刊介绍: The quarterly Polish Polar Research edited by the Committee on Polar Research of the Polish Academy of Sciences is an international journal publishing original research articles presenting the results of studies carried out in polar regions. All papers are peer-reviewed and published in English. The Editorial Advisory Board includes renowned scientist from Poland and from abroad.
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