Assessment of the molecular cytogenetic, morphometric and biochemical parameters of Deschampsia antarctica from its southern range limit in maritime Antarctic

IF 0.9 4区 地球科学 Q4 ECOLOGY Polish Polar Research Pub Date : 2018-01-01 DOI:10.24425/118759
D. Navrotska, Igor Andreev, A. Betekhtin, Magdalena Rojek, I. Parnikoza, G. Myryuta, O. Poronnik, N. Miryuta, Joanna SZYMANOWSKA-PU, Ka, V. Grakhov, R. Ivannikov, R. Hasterok, V. Kunakh
{"title":"Assessment of the molecular cytogenetic, morphometric and biochemical parameters of Deschampsia antarctica from its southern range limit in maritime Antarctic","authors":"D. Navrotska, Igor Andreev, A. Betekhtin, Magdalena Rojek, I. Parnikoza, G. Myryuta, O. Poronnik, N. Miryuta, Joanna SZYMANOWSKA-PU, Ka, V. Grakhov, R. Ivannikov, R. Hasterok, V. Kunakh","doi":"10.24425/118759","DOIUrl":null,"url":null,"abstract":"Different chromosomal forms of Deschampsia antarctica Desv. (Poaceae), including diploids (2n=26), hypotriploid (2n=36–38) and a genotype with an occasional occurrence of B chromosome (2n=26+0-1B) that originated from southern marginal populations (Argentine Islands region, maritime Antarctic) were studied using molecular cytogenetic, morphometric and biochemical methods. FISH analysis revealed variations in the number of rDNA sites between the diploid and hypotriploid plants. The genome size varied among plants with a different chromosome number and was on average 10.88 pg/2C for diploids and 16.46 pg/2C for hypotriploid. The mean values of leaf length of plants grown in vitro varied within a range of 5.23–9.56 cm. The total phenolic content ranged from 51.10 to 105.40 mg/g, and the total flavonoid content ranged from 1.22 to 4.67 mg/g. The amount of phenolic compounds did not differ significantly between the genotypes, while a variation in the flavonoid content was observed for L59 and DAR12. The diploids did not differ significantly among each other in terms of the number of rDNA loci, but differed slightly in their genome size. The individuals of DAR12 carrying B chromosome were similar to other diploids in terms of their genome size, but Daria Navrotska et al. 526 statistically differed in leaf length. The hypotriploid had both a greater number of rDNA sites and a larger genome size. No statistical correlations were observed between the genome size and leaf length or genome size and accumulation of phenolic and flavonoid compounds. The results of this study suggest that D. antarctica plants from the southern edge of the range are characterised by the heterogeneity of the studied parameters.","PeriodicalId":49682,"journal":{"name":"Polish Polar Research","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish Polar Research","FirstCategoryId":"89","ListUrlMain":"https://doi.org/10.24425/118759","RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 9

Abstract

Different chromosomal forms of Deschampsia antarctica Desv. (Poaceae), including diploids (2n=26), hypotriploid (2n=36–38) and a genotype with an occasional occurrence of B chromosome (2n=26+0-1B) that originated from southern marginal populations (Argentine Islands region, maritime Antarctic) were studied using molecular cytogenetic, morphometric and biochemical methods. FISH analysis revealed variations in the number of rDNA sites between the diploid and hypotriploid plants. The genome size varied among plants with a different chromosome number and was on average 10.88 pg/2C for diploids and 16.46 pg/2C for hypotriploid. The mean values of leaf length of plants grown in vitro varied within a range of 5.23–9.56 cm. The total phenolic content ranged from 51.10 to 105.40 mg/g, and the total flavonoid content ranged from 1.22 to 4.67 mg/g. The amount of phenolic compounds did not differ significantly between the genotypes, while a variation in the flavonoid content was observed for L59 and DAR12. The diploids did not differ significantly among each other in terms of the number of rDNA loci, but differed slightly in their genome size. The individuals of DAR12 carrying B chromosome were similar to other diploids in terms of their genome size, but Daria Navrotska et al. 526 statistically differed in leaf length. The hypotriploid had both a greater number of rDNA sites and a larger genome size. No statistical correlations were observed between the genome size and leaf length or genome size and accumulation of phenolic and flavonoid compounds. The results of this study suggest that D. antarctica plants from the southern edge of the range are characterised by the heterogeneity of the studied parameters.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
南极海洋南缘南极德尚蚤的分子细胞遗传学、形态计量学和生化参数评价
南极斑蝶的不同染色体形态。采用分子细胞遗传学、形态计量学和生物化学等方法,对阿根廷群岛、南极海域等南部边缘种群中二倍体(2n=26)、半三倍体(2n=36 ~ 38)和一个偶有B染色体的基因型(2n=26+0 ~ 1b)进行了研究。FISH分析显示二倍体和半三倍体植株的rDNA位点数量存在差异。不同染色体数目的植株基因组大小不同,二倍体平均为10.88 pg/2C,半三倍体平均为16.46 pg/2C。离体植株叶长平均值在5.23 ~ 9.56 cm之间。总酚含量为51.10 ~ 105.40 mg/g,总黄酮含量为1.22 ~ 4.67 mg/g。酚类化合物含量在不同基因型间差异不显著,而类黄酮含量在L59和DAR12基因型间存在差异。二倍体在rDNA位点数量上差异不显著,但在基因组大小上差异不大。携带B染色体的DAR12个体在基因组大小上与其他二倍体相似,但Daria Navrotska等526在叶片长度上存在统计学差异。亚三倍体具有更多的rDNA位点和更大的基因组大小。基因组大小与叶片长度、基因组大小与酚类化合物和类黄酮化合物积累均无统计学相关性。本研究结果表明,南缘的南极D. antarctica植物具有研究参数的异质性特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Polish Polar Research
Polish Polar Research ECOLOGY-GEOSCIENCES, MULTIDISCIPLINARY
CiteScore
2.00
自引率
7.70%
发文量
0
审稿时长
>12 weeks
期刊介绍: The quarterly Polish Polar Research edited by the Committee on Polar Research of the Polish Academy of Sciences is an international journal publishing original research articles presenting the results of studies carried out in polar regions. All papers are peer-reviewed and published in English. The Editorial Advisory Board includes renowned scientist from Poland and from abroad.
期刊最新文献
Novel cold-active amylase-producing bacterium from Chukchi Sea and its enzyme properties Salutogenic outcomes and their personality predictors in participants of year-long Antarctic expeditions History of female Polish pioneers and expeditioners in polar regions 150020 149207
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1