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Novel cold-active amylase-producing bacterium from Chukchi Sea and its enzyme properties 来自楚科奇海的新型冷活性淀粉酶产生菌及其酶特性
IF 0.9 4区 地球科学 Q4 ECOLOGY Pub Date : 2024-07-23 DOI: 10.24425/ppr.2024.150879
Min Ju Kim, Jin A Kim, H. Park, J. Yim, Il-Chan Kim, S. Han
A cold-active amylase was purified from Alteromonas sp. KS7913 isolated from the Chukchi Sea in the Arctic Ocean. After purification with use of ammonium sulfate precipitation, phenyl column chromatography, and size exclusion chromatography, 200.34 U mg−1 of purified amylase was obtained. The final yield was 3.4%, and the activity was 5.7-fold higher than that of the initial culture broth. KS7913 origin amylase showed a molecular weight of 70 kDa and optimal activity at 25℃, pH 7.0 in Tris-HCl buffer. The amylase was highly active, especially at 5℃, and maintained stability at basic conditions below 25℃. Copper and zinc ions inhibited enzyme activity, whereas manganese, barium, and calcium ions exhibited positive effects. This activity was maintained even in the presence of alcohol. The findings of this study supplement our understanding of cold-active amylases, and may have practical applications in low-temperature industries.
从北冰洋楚科奇海分离的 Alteromonas sp. KS7913 中纯化出一种冷活性淀粉酶。经硫酸铵沉淀、苯基柱层析和尺寸排除层析纯化后,获得了 200.34 U mg-1 的纯化淀粉酶。最终产率为 3.4%,活性是初始培养液的 5.7 倍。KS7913 起源的淀粉酶分子量为 70 kDa,在 25℃、pH 7.0 的 Tris-HCl 缓冲液中具有最佳活性。淀粉酶的活性很高,尤其是在 5℃时,并在 25℃以下的碱性条件下保持稳定。铜离子和锌离子抑制酶的活性,而锰、钡和钙离子则有积极作用。即使在酒精存在的情况下,酶的活性也能保持。这项研究的结果补充了我们对低温活性淀粉酶的认识,并可能在低温工业中得到实际应用。
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引用次数: 0
Salutogenic outcomes and their personality predictors in participants of year-long Antarctic expeditions 为期一年的南极探险参与者的致乐结果及其人格预测因素
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2024-05-17 DOI: 10.24425/ppr.2024.150021
Oleg Kokun, Larysa Bakhmutova
Prolonged stays in extreme living and working conditions at Antarctic stations can result in both negative psychological manifestations and possible positive, salutogenic effects. The aim of this study was to check an assumption about existing salutogenic outcomes and their personality predictors in expeditioners who participated in year-long expeditions. We examined 62 expeditioners who participated in expeditions to the Ukrainian Antarctic Akademik Vernadsky station between 1996 and 2021, including 59 men and three women aged 27 to 68 years. We used the Posttraumatic Growth Inventory—Expanded, the General Self-Efficacy Scale, the Eysenck Personality Questionnaire, the Sixteen Personality Factor Questionnaire and the Professional Hardiness Questionnaire. The majority of expeditioners (55–71%, on various grounds) recorded personal growth following Antarctic deployment, at a level from moderate to high. Based on personality characteristics diagnosed in the abovementioned questionnaires, we created an informative prognostic model explaining 30–45% of the variation in several indicators of expeditioners’ post-expedition growth. The most important predictors of expeditioners’ post-expedition growth were indicators of professional hardiness. Our findings provide additional opportunities to improve psychological evaluation and training for Antarctic expedition personnel.
在南极考察站长期处于极端的生活和工作条件下,既可能导致负面的心理表现,也可能产生积极的致盐效应。本研究的目的是检验参加为期一年探险的探险队员现有的致盐结果及其人格预测因素的假设。我们调查了 1996 年至 2021 年期间参加乌克兰南极阿卡德米克-维尔纳茨基站探险的 62 名探险队员,其中包括 59 名男性和 3 名女性,年龄在 27 岁至 68 岁之间。我们使用了创伤后成长量表(Posttraumatic Growth Inventory-Expanded)、一般自我效能量表(General Self-Efficacy Scale)、艾森克人格问卷(Eysenck Personality Questionnaire)、十六种人格因素问卷(Sixteen Personality Factor Questionnaire)和职业坚韧性问卷(Professional Hardiness Questionnaire)。大多数探险队员(55-71%,基于各种理由)在南极部署后都有个人成长记录,成长程度从中度到高度不等。根据上述问卷调查所显示的个性特征,我们创建了一个信息丰富的预测模型,可以解释远征队员在远征后成长的若干指标中 30% 至 45% 的变化。预测远征队员远征后成长的最重要指标是职业坚韧性指标。我们的研究结果为改进南极考察人员的心理评估和培训提供了更多机会。
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引用次数: 0
History of female Polish pioneers and expeditioners in polar regions 波兰女性极地开拓者和探险家的历史
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2024-05-17 DOI: 10.24425/ppr.2024.150022
Dagmara Bożek, Joanna Plenzler, Katarzyna Greń
In the paper, we present a gradual transition from domination of men to equal treatment for men and women during the several decades of Polish expeditions to scientific stations in the Arctic and the Antarctic. This research has been based on the data available in the archives and at the database created within the project Female polar explorers (Polarniczki in Polish), which contains information about the number of female participants of expeditions to Polish research stations. Women participated in the seasonal expeditions almost from the very beginning when Polish research stations were established in 1970s and 1980s, but a significant increase of female participants is visible especially at year-round stations in the second decade of the 21st century. In 1984, the first Polish female scientist overwintered in the Antarctic and in 1996 in the Arctic. In 1986, the first woman became a leader of a summer scientific expedition to Spitsbergen, and in 1991 a first woman became the leader of a year-long expedition to Arctowski Station. In the presented database, there are 825 records, that correspond to 417 surnames of female participants in Polish polar expeditions, 145 participated in more than one expedition. Women who were working at Polish polar research stations represented a variety of occupations, but most of them were scientists or combined science and station service. The collected data clearly show that the days when the presence of women at polar stations was unwelcome are a thing of the past.
在本文中,我们介绍了波兰在北极和南极科学考察站进行考察的几十年间,从男性主导逐渐过渡到男女平等的情况。这项研究以档案中的数据和在 "女性极地探险家"(波兰语为 Polarniczki)项目中创建的数据库为基础,该数据库包含了波兰研究站探险活动的女性参与者人数信息。几乎从 20 世纪 70 年代和 80 年代波兰研究站建立之初,女性就参加了季节性探险活动,但在 21 世纪第二个十年,女性参与者的人数显著增加,尤其是在常年研究站。1984 年,第一位波兰女科学家在南极越冬,1996 年在北极越冬。1986 年,第一位女性成为斯匹次卑尔根夏季科学考察队的队长,1991 年,第一位女性成为阿克托夫斯基站一年考察队的队长。在所提供的数据库中,有 825 条记录,对应 417 个参加波兰极地考察的女性姓氏,其中 145 人参加了一次以上的考察。在波兰极地考察站工作的女性代表了不同的职业,但她们中的大多数是科学家或科学与考察站服务相结合的人员。收集到的数据清楚地表明,极地考察站不欢迎女性的时代已经成为过去。
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引用次数: 0
150020 150020
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2024-03-21 DOI: 10.24425/ppr.2024.150020
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引用次数: 0
149207 149207
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2024-01-25 DOI: 10.24425/ppr.2024.149207
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引用次数: 0
In Memoriam Professor Aleksander Guterch (1936–2023) an outstanding Polish geophysicist and polar researcher 悼念杰出的波兰地球物理学家和极地研究者 Aleksander Guterch 教授(1936-2023 年
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2024-01-25 DOI: 10.24425/ppr.2024.149208
T. Janik, W. Czuba
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引用次数: 0
Importance of snow as component of surface mass balance of Arctic glacier (Hansbreen, southern Spitsbergen) 雪作为北极冰川表面物质平衡组成部分的重要性(汉斯布林,斯匹次卑尔根南部)
IF 1.3 4区 地球科学 Q2 Environmental Science Pub Date : 2023-11-06 DOI: 10.24425/PPR.2019.130901
Aleksander Uszczyk, M. Grabiec, Michał Laska, M. Kuhn, D. Ignatiuk
: Snowmelt is a very important component of freshwater resources in the polar environment. Seasonal fluctuations in the water supply to glacial drainage systems influence glacier dynamics and indirectly affect water circulation and stratification in fjords. Here, we present spatial distribution of the meltwater production from the snow cover on Hansbreen in southern Spitsbergen. We estimated the volume of freshwater coming from snow deposited over this glacier. As a case study, we used 2014 being one of the warmest season in the 21 st century. The depth of snow cover was measured using a high frequency Ground Penetrating Radar close to the maximum stage of accumulation. Simultaneously, a series of studies were conducted to analyse the structure of the snowpack and its physical properties in three snow pits in different glacier elevation zones. These data were combined to construct a snow density model for the entire glacier, which together with snow depth distribution represents essential parameters to estimate glacier winter mass balance. A temperature index model was used to calculate snow ablation, applying an average temperature lapse rate and surface elevation changes. Applying variable with altitude degree day factor, we estimated an average daily rate of ablation between 0.023 m d -1 °C -1 (for the ablation zone) and 0.027 m d -1 °C -1 (in accumulation zone). This melting rate was further validated by direct ablation data at reference sites on the glacier. An average daily water production by snowmelt in 2014 ablation season was 0.0065 m w.e. (water equivalent) and 41.52·10 6 m 3 of freshwater in total. This ablation concerned 85.5% of the total water accumulated during winter in snow cover. Extreme daily melting exceeded 0.020 m w.e. in June and September 2014 with a maximum on 6 th July 2014 (0.027 m w.e.). The snow cover has completely disappeared at the end of ablation season on 75.8% of the surface of Hansbreen.
在极地环境中,融雪是淡水资源的重要组成部分。冰川排水系统供水的季节性波动影响冰川动力学,并间接影响峡湾的水循环和分层。本文研究了斯匹次卑尔根南部汉斯布林地区积雪融水产量的空间分布。我们估计了冰川上积雪的淡水量。作为一个案例研究,我们用2014年作为21世纪最温暖的季节之一。利用高频探地雷达在最大积雪阶段附近测量积雪深度。同时,对不同冰川高程带3个雪坑的积雪结构及其物理性质进行了分析。结合这些数据构建了整个冰川的雪密度模型,该模型与雪深分布是估算冰川冬季物质平衡的重要参数。利用平均温度递减率和地表高程变化,采用温度指数模型计算积雪消融。应用高度日因子,我们估计平均日消融速率在0.023 m d -1°C -1(消融区)和0.027 m d -1°C -1(堆积区)之间。冰川参考点的直接消融数据进一步证实了这一融化速度。2014年消融季融雪日平均出水量为0.0065 m w.e.(水当量),淡水总量为41.52·10.6 m³。消融量占积雪冬季累计水量的85.5%。2014年6月和9月极端日融雪量超过0.020 m w.e, 7月6日最大(0.027 m w.e)。消融季结束时,汉山75.8%的地表积雪完全消失。
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引用次数: 6
Growth form classification for sessile suspension feeders and their distribution in Antarctic fjord, King George Island 乔治王岛南极峡湾固定式悬浮食饵的生长形态分类及其分布
4区 地球科学 Q2 Environmental Science Pub Date : 2023-11-06 DOI: 10.24425/ppr.2022.140364
: Sessile suspension feeders depend primarily on availability of a space to settle and access to the water column. Their sessile nature incapacitates displacement during disturbances thus they rely on their morphology to overcome selective processes. We classified the assemblage of SSF from Mackellar Inlet (King George Island, Antarctica) according to their growth forms (GF) and epibiotic association type, the latter based on direct observation of the epibiotic behaviour of every individual. Organisms that did not comply with any previously established GF were grouped into ‘other GF’. Sampling stations were distributed across the fjord following a gradient based primarily on the distance to Domeyko Glacier (inner, middle, outer sections). Seven GF were recognised in the glaciomarine fjord: tree, bush, stalk, mound, flat, runner, and sheet. Four types of epibiotic associations were identified: basibiont, both facultative epibiont and basibiont, facultative epibiont (non-basibiont), and epibiont. Our results showed that the tree GF were found in the inner and middle sections, mound in middle and outer, and flat across all fjord sections. These GF enhanced GF-diversity since they constituted additional substrate for most of the ‘other GF’ which had primarily an epibiotic strategy. Contrastingly, bush, runner and stalk GF were only found in the outer section of the fjord, thus the most distanced from periglacial disturbances. The GF distribution was consistent with distance to glacier, both in number and strategies. These results highlight the potentialities of the morpho-functional classification applied to Antarctic sessile suspension feeders to help understand their distribution based on adaptive capabilities.
{"title":"Growth form classification for sessile suspension feeders and their distribution in Antarctic fjord, King George Island","authors":"","doi":"10.24425/ppr.2022.140364","DOIUrl":"https://doi.org/10.24425/ppr.2022.140364","url":null,"abstract":": Sessile suspension feeders depend primarily on availability of a space to settle and access to the water column. Their sessile nature incapacitates displacement during disturbances thus they rely on their morphology to overcome selective processes. We classified the assemblage of SSF from Mackellar Inlet (King George Island, Antarctica) according to their growth forms (GF) and epibiotic association type, the latter based on direct observation of the epibiotic behaviour of every individual. Organisms that did not comply with any previously established GF were grouped into ‘other GF’. Sampling stations were distributed across the fjord following a gradient based primarily on the distance to Domeyko Glacier (inner, middle, outer sections). Seven GF were recognised in the glaciomarine fjord: tree, bush, stalk, mound, flat, runner, and sheet. Four types of epibiotic associations were identified: basibiont, both facultative epibiont and basibiont, facultative epibiont (non-basibiont), and epibiont. Our results showed that the tree GF were found in the inner and middle sections, mound in middle and outer, and flat across all fjord sections. These GF enhanced GF-diversity since they constituted additional substrate for most of the ‘other GF’ which had primarily an epibiotic strategy. Contrastingly, bush, runner and stalk GF were only found in the outer section of the fjord, thus the most distanced from periglacial disturbances. The GF distribution was consistent with distance to glacier, both in number and strategies. These results highlight the potentialities of the morpho-functional classification applied to Antarctic sessile suspension feeders to help understand their distribution based on adaptive capabilities.","PeriodicalId":49682,"journal":{"name":"Polish Polar Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135636268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increase of tourist traffic on Spitsbergen: An environmental challenge or chance for progress in the region? 斯匹次卑尔根岛旅游客流量增加:环境挑战还是该地区进步的机遇?
4区 地球科学 Q2 Environmental Science Pub Date : 2023-11-06 DOI: 10.24425/ppr.2021.136601
: The Arctic region is under considerable pressure from the rapidly growing global tourism industry. The goal of this article is to present the current state of tourism in Spitsbergen, including the results of empirical research about the type of tourists and their preferences in organizing a trip, i.e. motivation, choice of attractions, etc . The survey in Longyearbyen town was conducted in 2018 and 2019 during two summer and two winter trips. As a result, a total of n=214 questionnaires were collected, presenting interesting insights into tourists’ profiles and their motivation in coming to the island. Based on the results, the Arctic tourism sector is characterized by seasonality, which significantly affects the distribution of tourist arrivals throughout the year. During the last 15 years, Spitsbergen island noticed the greatest boom in tourism linked to the development of transport infrastructure (new ports and airports) and accommodation (hotels). From one side, the tourism industry contributes significantly to employment in Longyearbyen, still from the other side, it burdens the local community and challenges their lives due to increased pollution of air, water and ground. The increased tourism also poses serious threats to the natural environment, which is already under pressure due to global warming resulting in melting Arctic glaciers and increased sea level. Therefore, it is crucial to implement new regulations to control and manage the development of tourism while protecting nature and its residents.
{"title":"Increase of tourist traffic on Spitsbergen: An environmental challenge or chance for progress in the region?","authors":"","doi":"10.24425/ppr.2021.136601","DOIUrl":"https://doi.org/10.24425/ppr.2021.136601","url":null,"abstract":": The Arctic region is under considerable pressure from the rapidly growing global tourism industry. The goal of this article is to present the current state of tourism in Spitsbergen, including the results of empirical research about the type of tourists and their preferences in organizing a trip, i.e. motivation, choice of attractions, etc . The survey in Longyearbyen town was conducted in 2018 and 2019 during two summer and two winter trips. As a result, a total of n=214 questionnaires were collected, presenting interesting insights into tourists’ profiles and their motivation in coming to the island. Based on the results, the Arctic tourism sector is characterized by seasonality, which significantly affects the distribution of tourist arrivals throughout the year. During the last 15 years, Spitsbergen island noticed the greatest boom in tourism linked to the development of transport infrastructure (new ports and airports) and accommodation (hotels). From one side, the tourism industry contributes significantly to employment in Longyearbyen, still from the other side, it burdens the local community and challenges their lives due to increased pollution of air, water and ground. The increased tourism also poses serious threats to the natural environment, which is already under pressure due to global warming resulting in melting Arctic glaciers and increased sea level. Therefore, it is crucial to implement new regulations to control and manage the development of tourism while protecting nature and its residents.","PeriodicalId":49682,"journal":{"name":"Polish Polar Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135636745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Limno-terrestrial diatom flora in two stream valleys near Arctowski Station, King George Island, Antarctica 南极乔治国王岛阿尔托斯基站附近的两个溪谷中的林诺-陆生硅藻区系
4区 地球科学 Q2 Environmental Science Pub Date : 2023-11-06 DOI: 10.24425/ppr.2020.134793
{"title":"Limno-terrestrial diatom flora in two stream valleys near Arctowski Station, King George Island, Antarctica","authors":"","doi":"10.24425/ppr.2020.134793","DOIUrl":"https://doi.org/10.24425/ppr.2020.134793","url":null,"abstract":"","PeriodicalId":49682,"journal":{"name":"Polish Polar Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135636976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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Polish Polar Research
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