Light-induced Membrane Hyperpolarization Promotes Osteoblast Differentiation in MC3T3 Osteoblast-like Cells

IF 0.3 4区 医学 Q4 ENGINEERING, BIOMEDICAL Journal of Hard Tissue Biology Pub Date : 2021-01-01 DOI:10.2485/jhtb.30.347
T. Notomi, R. Kobayashi, M. Otsuka, Chie Kise, Y. Momota, Y. Ezura, T. Kawazoe
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引用次数: 1

Abstract

Bone mass is regulated by bone remodeling, which involves bone formation by osteoblasts and bone resorption by osteoclasts. To prevent and treat bone loss, a basic understanding of the mechanism of bone formation is essential, including osteoblast differentiation, and its responses to mechanical stimuli that induce changes in membrane potential. During osteoblast differentiation, hyperpolarized membrane potential was observed. To understand osteoblast differentiation in response to membrane hyperpolarization, as well as the long-term effects of changes in membrane potential, we developed a light-controllable membrane potential system in MC3T3-E1 osteoblast-like cells by stably expressing the light-driven outward proton pump, archaerhodopsin-3. Archaerhodopsin-3 activation by yellow-green light hyperpolarizes the cell membrane Light-induced hyperpolarization accelerated osteoblast mineralization, as assessed by Alizarin Red staining, alkaline phosphatase activity, and expression levels of osteoblast differentiation markers. This promotion of osteoblast mineralization is related to voltage-gated Ca channels. Our study revealed a novel role of membrane potential in non-excitable osteoblast-like cells.
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光诱导膜超极化促进MC3T3成骨细胞样细胞的成骨分化
骨量受骨重塑调节,骨重塑包括成骨细胞的骨形成和破骨细胞的骨吸收。为了预防和治疗骨质流失,对骨形成机制的基本了解是必不可少的,包括成骨细胞分化及其对诱导膜电位变化的机械刺激的反应。在成骨细胞分化过程中,观察到超极化膜电位。为了了解成骨细胞对膜超极化的分化反应,以及膜电位变化的长期影响,我们在MC3T3-E1成骨细胞样细胞中通过稳定表达光驱动的外向质子泵古藻紫红素-3,建立了光可控的膜电位系统。通过茜素红染色、碱性磷酸酶活性和成骨细胞分化标志物的表达水平来评估,光诱导的超极化加速了成骨细胞的矿化。这种促进成骨细胞矿化与电压门控钙通道有关。我们的研究揭示了膜电位在不可兴奋的成骨细胞样细胞中的新作用。
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来源期刊
Journal of Hard Tissue Biology
Journal of Hard Tissue Biology ENGINEERING, BIOMEDICAL-
CiteScore
0.90
自引率
0.00%
发文量
28
审稿时长
6-12 weeks
期刊介绍: Information not localized
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