Dong hwi Kim, J. Byun, Daeok Kim, Bosun Kim, W. Lim
{"title":"Geometric Evaluation of Biomimetic 3D Printed Rat Femur","authors":"Dong hwi Kim, J. Byun, Daeok Kim, Bosun Kim, W. Lim","doi":"10.2485/jhtb.32.133","DOIUrl":"https://doi.org/10.2485/jhtb.32.133","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atsushi Saito, Kanji Hirashima, R. Ikeda, Kiyomi Ogawa, Sumie Sato, Ken-ichiro Kikuchi
{"title":"Postnatal Changes in the Expression and Localization of Jmjd3 in the Mouse Submandibular Gland","authors":"Atsushi Saito, Kanji Hirashima, R. Ikeda, Kiyomi Ogawa, Sumie Sato, Ken-ichiro Kikuchi","doi":"10.2485/jhtb.32.83","DOIUrl":"https://doi.org/10.2485/jhtb.32.83","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shintaro Sakatoku, Y. Hayashi, Taku Futenma, R. Ishizaka, Chikako Gemba, H. Nawa
{"title":"Wnt10a Is a Candidate as a Non-Cellular Agent for Induction of Dental Pulp Regeneration with Dentine-Inducing Capacity.","authors":"Shintaro Sakatoku, Y. Hayashi, Taku Futenma, R. Ishizaka, Chikako Gemba, H. Nawa","doi":"10.2485/jhtb.32.41","DOIUrl":"https://doi.org/10.2485/jhtb.32.41","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68967223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Treatment of tumors in the head and neck region using anticancer agents or radiotherapy often causes oral mucositis, which is associated with severe mucosal pain. Hyaluronic acid (HA) is a moisturizing agent used clinically to treat oral mucositis; however, HA does not remain long-term in the mucositis region due to its liquid nature. We recently developed the original sheet form HA using a freeze-drying method. In this study, we evaluated the effect of topical application of the newly developed HA sheets on oral mucositis healing using an animal model. Experimental oral mucositis was induced in 6-week-old male Syrian hamsters by applying filter paper soaked with acetic acid to the cheek pouches. After determining the appropriate conditions for obtaining uniform mucositis region useful for evaluating the healing process, the effect of HA sheets on oral mucositis healing were evaluated macroscopically and histopathologically comparing three groups: an HA group, in which HA sheets were applied to the mucositis region from the second day after mucositis induction; a carboxymethylcellulose (CMC) control group treated with CMC sheets; and a control group in which no sheets were applied. Compared to the control group, the HA group exhibited significantly smaller mucositis region on Days 2-4. Histopathology analysis on Day 2 showed mild inflammatory cell infiltration, reduced edematous changes, partial regeneration of the oral mucosal epithelium, and parallel thick bundles of collagen fibers in the superficial layer in the HA group compared with the Control and CMC groups. In conclusion, a hamster oral mucositis model was established that allows for convenient evaluation of the healing process. Daily topical application of freeze-dried sheets of HA accelerated the healing of oral mucositis in this hamster model. These results suggest that HA sheets could be an effective material in the treatment of oral mucositis in cancer patients.
{"title":"Topical Administration of Freeze-Dried Sheets of Hyaluronic Acid Promotes the Healing of Oral Mucositis in a Hamster Model","authors":"Kei Suzuki-Mishima, Akiko Tanaka, Nahoko Kato-Kogoe, Koji Yamanegi, Azumi Hirata, Kunio Yoneto, Chika Yoneto, Wataru Hamada, Hidemasa Katsumi, Tomoyuki Furubayashi, Hiroyuki Nakano, Toshiyasu Sakane, Takaaki Ueno","doi":"10.2485/jhtb.32.223","DOIUrl":"https://doi.org/10.2485/jhtb.32.223","url":null,"abstract":"Treatment of tumors in the head and neck region using anticancer agents or radiotherapy often causes oral mucositis, which is associated with severe mucosal pain. Hyaluronic acid (HA) is a moisturizing agent used clinically to treat oral mucositis; however, HA does not remain long-term in the mucositis region due to its liquid nature. We recently developed the original sheet form HA using a freeze-drying method. In this study, we evaluated the effect of topical application of the newly developed HA sheets on oral mucositis healing using an animal model. Experimental oral mucositis was induced in 6-week-old male Syrian hamsters by applying filter paper soaked with acetic acid to the cheek pouches. After determining the appropriate conditions for obtaining uniform mucositis region useful for evaluating the healing process, the effect of HA sheets on oral mucositis healing were evaluated macroscopically and histopathologically comparing three groups: an HA group, in which HA sheets were applied to the mucositis region from the second day after mucositis induction; a carboxymethylcellulose (CMC) control group treated with CMC sheets; and a control group in which no sheets were applied. Compared to the control group, the HA group exhibited significantly smaller mucositis region on Days 2-4. Histopathology analysis on Day 2 showed mild inflammatory cell infiltration, reduced edematous changes, partial regeneration of the oral mucosal epithelium, and parallel thick bundles of collagen fibers in the superficial layer in the HA group compared with the Control and CMC groups. In conclusion, a hamster oral mucositis model was established that allows for convenient evaluation of the healing process. Daily topical application of freeze-dried sheets of HA accelerated the healing of oral mucositis in this hamster model. These results suggest that HA sheets could be an effective material in the treatment of oral mucositis in cancer patients.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Periosteal distraction is promising for augmenting new bone. This study aimed to develop a protocol for achieving stable bone augmentation. We applied mandibular alveolar ridge augmentation in beagles to test a trial distraction device. Premolars and molars were extracted bilaterally, and adjacent buccal cortical bone (3–4-mm thick) was removed to mimic atrophy. A 0.9-mm-diameter titanium screw was placed under the periosteum for later use to induce distraction. After healing (16 weeks), a titanium periosteal distraction device was applied on both sides in three beagles (six sides), and the periosteum was distracted laterally (0.5 mm/day for 6 days). One beagle was a control (two sides). No infection or displacement of the device was observed. All beagles were euthanized 10 weeks after distraction. Specimens were harvested and the mandibles were sectioned in half side was decalcified with formic acid and hematoxylin-eosin stained. Histological examination showed dense bone formation with a lamellar structure, with a bone width increase of 0.79 ± 0.11 mm compared with the control specimens. Bone formation can be induced by a periosteal distraction device, suggesting potential clinical utility.
{"title":"Histological Analysis on Decalcified Specimens in Alveolar Ridge Augmentation Using a Periosteal Distraction Device","authors":"Hiroki Inoue, Atsushi Abe, Hiroshi Furuta, Yoshihiko Sugita, Hatsuhiko Maeda","doi":"10.2485/jhtb.32.239","DOIUrl":"https://doi.org/10.2485/jhtb.32.239","url":null,"abstract":"Periosteal distraction is promising for augmenting new bone. This study aimed to develop a protocol for achieving stable bone augmentation. We applied mandibular alveolar ridge augmentation in beagles to test a trial distraction device. Premolars and molars were extracted bilaterally, and adjacent buccal cortical bone (3–4-mm thick) was removed to mimic atrophy. A 0.9-mm-diameter titanium screw was placed under the periosteum for later use to induce distraction. After healing (16 weeks), a titanium periosteal distraction device was applied on both sides in three beagles (six sides), and the periosteum was distracted laterally (0.5 mm/day for 6 days). One beagle was a control (two sides). No infection or displacement of the device was observed. All beagles were euthanized 10 weeks after distraction. Specimens were harvested and the mandibles were sectioned in half side was decalcified with formic acid and hematoxylin-eosin stained. Histological examination showed dense bone formation with a lamellar structure, with a bone width increase of 0.79 ± 0.11 mm compared with the control specimens. Bone formation can be induced by a periosteal distraction device, suggesting potential clinical utility.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135263080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Jia, Xinyue Li, Yongqiang Ma, Zhi-Tao Wang, Jian Kang
{"title":"Comparison of the Clinical and Radiographical Outcomes of Regenerative Surgery and Open Flap Debridement in the Treatment of Shallow No-Contentive Periodontal Infra-Bony Defects","authors":"Yan Jia, Xinyue Li, Yongqiang Ma, Zhi-Tao Wang, Jian Kang","doi":"10.2485/jhtb.32.143","DOIUrl":"https://doi.org/10.2485/jhtb.32.143","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Topical Application of Microneedling and a Hyaluronic Acid Gel Promotes Periodontal Soft Tissue Regeneration in Vivo","authors":"Lin Fan, Daqing He, Song Ren, Jiang Sun","doi":"10.2485/jhtb.32.167","DOIUrl":"https://doi.org/10.2485/jhtb.32.167","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68967036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dental pulp stem cells (DPSCs) invoke wide interest due to their roles in in dentin regeneration and repair, and more research is required to illustrate the molecular basis. In this study, we induced osteogenic differentiation in human DPSCs to investigate the role of interferon-induced transmembrane (IFITM) family in osteogenic differentiation. Our results demonstrated that genes of the IFITM family, IFITM1, IFITM3, and IFITM5 were upregulated during the osteogenic differentiation of DPSCs. Loss-of-function and gain-of-function assays indicated that IFITM3 knockdown suppressed osteogenic differentiation, assessed by the reduction of alkaline phosphatase (ALP) activity, nodule mineralization, and the expressions of several osteogenic differentiation-associated genes; in contrast, IFITM3 overexpression increased ALP activity, nodule mineralization, and the expressions of osteogenic differentiation-associated genes. Further investigation revealed that IFITM3 was involved in the regulation of mitogen-activated protein kinase (MAPK) signaling during osteogenic differentiation, and pharmacological inhibition of both ERK and p38 suppressed the effects of IFITM3 overexpression in osteogenic differentiation. These results suggest that IFITM3 promotes osteogenic differentiation of DPSCs by regulating MAPK signaling. Our findings enhance the existing knowledge on the role of IFITM family in osteogenic differentiation and the molecular basis of IFITM3.
{"title":"IFITM3 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells by Modulating MAPK Signaling","authors":"Wanjun Xu, Yina Wang","doi":"10.2485/jhtb.32.205","DOIUrl":"https://doi.org/10.2485/jhtb.32.205","url":null,"abstract":"Dental pulp stem cells (DPSCs) invoke wide interest due to their roles in in dentin regeneration and repair, and more research is required to illustrate the molecular basis. In this study, we induced osteogenic differentiation in human DPSCs to investigate the role of interferon-induced transmembrane (IFITM) family in osteogenic differentiation. Our results demonstrated that genes of the IFITM family, IFITM1, IFITM3, and IFITM5 were upregulated during the osteogenic differentiation of DPSCs. Loss-of-function and gain-of-function assays indicated that IFITM3 knockdown suppressed osteogenic differentiation, assessed by the reduction of alkaline phosphatase (ALP) activity, nodule mineralization, and the expressions of several osteogenic differentiation-associated genes; in contrast, IFITM3 overexpression increased ALP activity, nodule mineralization, and the expressions of osteogenic differentiation-associated genes. Further investigation revealed that IFITM3 was involved in the regulation of mitogen-activated protein kinase (MAPK) signaling during osteogenic differentiation, and pharmacological inhibition of both ERK and p38 suppressed the effects of IFITM3 overexpression in osteogenic differentiation. These results suggest that IFITM3 promotes osteogenic differentiation of DPSCs by regulating MAPK signaling. Our findings enhance the existing knowledge on the role of IFITM family in osteogenic differentiation and the molecular basis of IFITM3.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"miR-141 Improve Osteoporosis by Promoting Osteoblast Differentiation through Targeting RICTOR","authors":"Siyuan Chen, Jinsong Wei, Weixiong Guo","doi":"10.2485/jhtb.32.111","DOIUrl":"https://doi.org/10.2485/jhtb.32.111","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayako Miyasaka, R. Ikeda, Kanji Hirashima, Sumie Sato, Ken-ichiro Kikuchi, T. Miyasaka, T. Satomi
{"title":"Examination of Changes in the Remaining Submandibular Gland after Resection of the Contralateral Salivary Gland","authors":"Ayako Miyasaka, R. Ikeda, Kanji Hirashima, Sumie Sato, Ken-ichiro Kikuchi, T. Miyasaka, T. Satomi","doi":"10.2485/jhtb.32.57","DOIUrl":"https://doi.org/10.2485/jhtb.32.57","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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