SSR Genotyping of Wild Grape Species and Grape Cultivars of Vitis vinifera and V. vinifera × V. labrusca

N. Goto-Yamamoto, A. Azuma, N. Mitani, S. Kobayashi
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引用次数: 9

Abstract

To determine the relationship among wild grape species and grape cultivars, genotyping with 8 SSR loci was carried out using a wide range of grape species and cultivars, i.e., North American species, East Asian species, cultivars of Vitis vinifera, and those of V. vinifera × V. labrusca. SSR genotyping showed that wild grape species are more highly diverse than cultivated grapes. Principal coordinate analysis based on the distance (1-proportion of shared alleles) among accessions showed a clear separation between wild and cultivated grapes. A native Japanese cultivar, ‘Koshu’, and a Chinese cultivar, ‘Niunai’, were plotted among the cultivars of V. vinifera, even though these two Far East cultivars have some unique alleles.
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葡萄品种和葡萄品种的SSR基因分型研究
为确定野生葡萄品种与栽培品种的亲缘关系,利用北美品种、东亚品种、葡萄品种、葡萄×葡萄品种等8个SSR位点进行了基因分型。SSR基因分型表明,野生葡萄品种多样性高于栽培葡萄品种。基于距离(共享等位基因的1-比例)的主坐标分析表明,野生葡萄和栽培葡萄之间存在明显的分离。一个日本本土栽培品种“Koshu”和一个中国栽培品种“Niunai”被绘制在葡萄品种中,尽管这两个远东栽培品种具有一些独特的等位基因。
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