SEASONAL VARIATION IN ROOTING BEHAVIOUR OF GINKGO BILOBA L. CUTTINGS

N. Bhattarai, S. D. Joshi
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引用次数: 2

Abstract

Propagation by cuttings is an effective method for ex-situ conservation of plants. In the present study, propagation of Ginkgo biloba L. was conducted using hardwood and semi hard wood cuttings. The cuttings from basal part of the tree were selected for the experiment and planted during rainy (i.e. July) and summer season (i.e. April) at Central Department of Botany, T.U. Cuttings beds were prepared using 3:1 sand and soil. Different concentration of auxins such as IAA, NAA and IBA were found effective in inducing rooting. During July, the stem cuttings treated with 1000 ppm IAA for 30 minutes induced highest percentage (90 %) and statistically signifi cant (P< 0.05) rooting while those treated with same concentration and time induced only 40% rooting during April. Like wise other auxins NAA and IBA induced more rooting during July and less rooting during April. Cuttings planted as control, during July showed 10 % rooting while during April did not show any rooting. Among all the auxins studied, IAA hormone and rainy season was found to be the most effective for rooting of G. biloba cuttings. From the anatomical study it was revealed that vascular cambium, secondary phloem, cortical cells and pericycle cells took part on rooting. Successfully rooted cuttings were then transferred to the natural soil condition. J. Nat. Hist. Mus. Vol. 26, 2012: 175-180
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银杏扦插生根行为的季节变化
扦插繁殖是植物迁地保护的一种有效方法。本研究采用硬木和半硬木扦插繁殖银杏。试验选用树基部插枝,于雨季(即7月)和夏季(即4月)在清华大学中央植物学系进行扦插,扦插床采用3:1沙土配制。不同浓度的生长素(IAA、NAA和IBA)均有诱导生根的效果。7月,1000ppm IAA处理30min的茎条生根率最高(90%),且有统计学意义(P< 0.05); 4月,相同浓度和时间处理的茎条生根率仅为40%。与其他生长素一样,NAA和IBA在7月的生根率较高,4月的生根率较低。对照扦插7月生根率为10%,4月则不生根。在所有生长素中,IAA激素和雨季对百叶插条生根最有效。解剖研究表明,维管形成层、次生韧皮部、皮质细胞和中柱鞘细胞参与了生根过程。然后将成功生根的插枝转移到自然土壤条件下。J.纳特。亩。Vol. 26, 2012: 175-180
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