{"title":"Effect of Mobile Phase ph on the Separation of Drugs using High-Performance Liquid Chromatography","authors":"V. Gupta, M. Mathew","doi":"10.3109/03639049509026647","DOIUrl":null,"url":null,"abstract":"AbstractWhen developing a HPLC assay method for drugs, the pH of the mobile phase can be effectively used as a tool to accomplish complete separation. Using two weak acids (hydrocortisone sodium succinate and methylprednisolone sodium succinate) and a weak base (promethazine hydrochloride), the changes in the chromatograms (retention times and separation of peaks) have been determined at 7 different pH values of a mobile phase with other conditions remaining the same. It has been shown that the retention times of weak acids will decrease with increasing pH values while those of weak bases will increase with increasing pH. The optimum pH value for the separation of these drugs has been determined to be 5.","PeriodicalId":11263,"journal":{"name":"Drug Development and Industrial Pharmacy","volume":"21 1","pages":"833-837"},"PeriodicalIF":2.4000,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/03639049509026647","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug Development and Industrial Pharmacy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3109/03639049509026647","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 7
Abstract
AbstractWhen developing a HPLC assay method for drugs, the pH of the mobile phase can be effectively used as a tool to accomplish complete separation. Using two weak acids (hydrocortisone sodium succinate and methylprednisolone sodium succinate) and a weak base (promethazine hydrochloride), the changes in the chromatograms (retention times and separation of peaks) have been determined at 7 different pH values of a mobile phase with other conditions remaining the same. It has been shown that the retention times of weak acids will decrease with increasing pH values while those of weak bases will increase with increasing pH. The optimum pH value for the separation of these drugs has been determined to be 5.
期刊介绍：
The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.
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