Analysis of the Mechanism of T Lymphocytes Promoting Immune Platelet Transfusion Refractoriness by Gene Chip Technique

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY Indian Journal of Pharmaceutical Sciences Pub Date : 2023-01-01 DOI:10.36468/pharmaceutical-sciences.spl.671
C. Song, Wen Liu, Jing Wang, Jing Liang
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Abstract

Technique The main objective of this study is to analyze the expression levels of messenger ribonucleic acid and long non-coding ribonucleic acid in patients with platelet transfusion refractoriness and reveal the mechanism of T lymphocytes in immune platelet transfusion refractoriness. The Agilent expression profile chip was used to detect the expression levels; gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis were performed on the differential genes to determine their main biological functions. Unsupervised hierarchical clustering was used to process differential genes and the differential genes among samples were represented in a heat map. The differentially expressed messenger ribonucleic acids and long non-coding ribonucleic acids in different groups were found as 720 and 1719 in normal control group vs. platelet transfusion effective group; 4254 and 12491 in normal control group vs. platelet transfusion ineffective group and 1806 and 6216 in platelet transfusion effective group vs. platelet transfusion ineffective group. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis were performed on differentially expressed genes, and the results were annotated to be related to T cells. The co-expression of the target gene Ras-related protein 1A and long non-coding transactive response deoxyribonucleic acid binding protein 2 was determined through the national center for biotechnology information database and the interaction between micro ribonucleic acid-4739 and Ras-related protein 1A was predicted using the starBase and TargetScan databases. T lymphocytes play an important role in immune platelet transfusion refractoriness and long non-coding transactive response deoxyribonucleic acid binding protein 2 may affect the differentiation of T lymphocytes and promote the occurrence of immune platelet transfusion refractoriness through micro ribonucleic acid-4739 targeting Ras-related protein 1A gene regulation.
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利用基因芯片技术分析T淋巴细胞促进免疫血小板输注难耐性的机制
技术本研究的主要目的是分析血小板输注难治性患者信使核糖核酸和长链非编码核糖核酸的表达水平,揭示T淋巴细胞在免疫血小板输注难治性中的作用机制。采用Agilent表达谱芯片检测表达水平;对差异基因进行基因本体和京都基因百科全书富集分析,确定其主要生物学功能。采用无监督分层聚类对差异基因进行处理,并用热图表示样本间的差异基因。正常对照组与血小板输注有效组的信使核糖核酸和长非编码核糖核酸差异表达量分别为720和1719;正常对照组与血小板输注无效组比较,分别为4254、12491例;血小板输注有效组与血小板输注无效组比较,分别为1806、6216例。对差异表达基因进行基因本体和京都基因百科全书及基因组富集分析,结果标注与T细胞相关。目的基因ras相关蛋白1A和长链非编码交互应答脱氧核糖核酸结合蛋白2的共表达通过国家生物技术信息中心数据库确定,微核糖核酸-4739与ras相关蛋白1A的相互作用通过starBase和TargetScan数据库预测。T淋巴细胞在免疫血小板输注难耐性中发挥重要作用,长链非编码交互反应脱氧核糖核酸结合蛋白2可能通过微核糖核酸-4739靶向ras相关蛋白1A基因调控,影响T淋巴细胞分化,促进免疫血小板输注难耐的发生。
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期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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