Polymorphism of HvDRO1 and HvqSOR1 associated with root growth angle in barley accessions

IF 1 Q3 PLANT SCIENCES Plant Root Pub Date : 2022-01-01 DOI:10.3117/plantroot.16.1
Y. Nakano, Junpei Konishi, Hirotake Ito, Tsuyoshi Tanaka, M. Seki, H. Aoki, T. Nagamine
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引用次数: 1

Abstract

: The root growth angle (RGA) is an important breeding target that confers high crop adaptability to deleterious environments. In barley, natural variations in RGA among accessions have been observed, but many of the genetic factors that cause of this variation remains unclear. In this study, we explored the orthologs of OsDRO1 ( DEEPER ROOTING 1 ) and OsqSOR1 (quantitative trait locus for SOIL SURFACE ROOTING 1) , which play a critical role in RGA regulation in rice, from barley genome and analyzed the polymorphisms of these genes among barley accessions. BLASTP search detected putative orthologs of OsDRO1 and OsqSOR1 in barley (HvDRO1 and HvqSOR1) with more than 60% amino acid similarity. Sequence analysis identified SNPs causing mis-splicing and nonsynonymous amino acid substitution in HvDRO1 and HvqSOR1 , respectively. These SNPs were associated with RGA variation among the 47 barley accessions. Phylogenetic analysis using the 105 barley accessions revealed that the alleles of HvDRO1 and HvqSOR1 are related to the genetic background of the accessions. Further-more, the mutant allele of HvDRO1 is mainly shared in the Hokuriku/Nagano subpopulation, suggesting that the mutant allele is involved in local adaptation of barley cultivars to the soil environment of the region. Our findings suggest that the polymorphisms of HvDRO1 and HvqSOR1 are possible determinants of RGA variation in barley, at least in Japanese accessions, and provide information on allelic variants of the genes for marker-assisted selection to genetic improvement of RGA of barley.
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大麦种质中与根生长角相关的HvDRO1和HvqSOR1多态性
根系生长角(RGA)是一项重要的育种指标,它能提高作物对有害环境的适应性。在大麦中,已观察到不同品种间RGA的自然变异,但导致这种变异的许多遗传因素仍不清楚。本研究从大麦基因组中寻找了在水稻RGA调控中起关键作用的OsDRO1 (DEEPER生根1)和OsqSOR1 (SOIL SURFACE生根数量性状位点1)的同源基因,并分析了这两个基因在大麦材料中的多态性。BLASTP搜索发现大麦中OsDRO1和OsqSOR1的推定同源基因(HvDRO1和HvqSOR1)具有超过60%的氨基酸相似性。序列分析发现,在HvDRO1和HvqSOR1中分别存在导致错误剪接和非同义氨基酸取代的snp。这些snp与47份大麦材料的RGA变异有关。对105份大麦材料的系统发育分析表明,HvDRO1和HvqSOR1等位基因与材料的遗传背景有关。此外,HvDRO1突变等位基因主要在北陆/长野亚群中共享,表明该突变等位基因参与了大麦品种对该地区土壤环境的局部适应。我们的研究结果表明,HvDRO1和HvqSOR1的多态性可能是大麦(至少在日本品种中)RGA变异的决定因素,并为大麦RGA遗传改良的标记辅助选择提供了基因等位变异的信息。
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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