{"title":"Comparison of effect of salt stress on the cell death in seminal root and lateral root of rye seedlings by the modified TUNEL method","authors":"Atsushi Ogawa, S. Shirado, K. Toyofuku","doi":"10.3117/PLANTROOT.6.5","DOIUrl":null,"url":null,"abstract":"Cell death in the lateral root tip of rye seedlings under salt stress conditions was analyzed quantitatively by the modified terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method and the frequency of cell death was compared in the seminal root tip. There were no significant differences in total root length and the number of root tips among control, 10 mM and 100 mM NaCl treatments, although the root elongation and initiation was inhibited in the 250 mM NaCl treatment. The frequency of cell death was increased in 100 mM and 250 mM NaCl treatments compared with the control, significantly. There was no significant difference in the frequency of cell death between seminal root and lateral root in all stress treatments. Moreover, technical advantage of the modified TUNEL method was discussed by comparing with the classical TUNEL method.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"6 1","pages":"5-9"},"PeriodicalIF":1.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.6.5","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Root","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3117/PLANTROOT.6.5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 4
Abstract
Cell death in the lateral root tip of rye seedlings under salt stress conditions was analyzed quantitatively by the modified terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method and the frequency of cell death was compared in the seminal root tip. There were no significant differences in total root length and the number of root tips among control, 10 mM and 100 mM NaCl treatments, although the root elongation and initiation was inhibited in the 250 mM NaCl treatment. The frequency of cell death was increased in 100 mM and 250 mM NaCl treatments compared with the control, significantly. There was no significant difference in the frequency of cell death between seminal root and lateral root in all stress treatments. Moreover, technical advantage of the modified TUNEL method was discussed by comparing with the classical TUNEL method.
采用改良的末端脱氧核苷酸转移酶(TdT)介导的dUTP缺口末端标记(TUNEL)方法，定量分析了盐胁迫条件下黑麦幼苗侧根尖的细胞死亡情况，并比较了种子根尖细胞死亡的频率。对照、10 mM和100 mM NaCl处理的总根长和根尖数量无显著差异，但250 mM NaCl处理的根伸长和萌发受到抑制。与对照相比，100 mM和250 mM NaCl处理的细胞死亡频率显著增加。在所有胁迫处理下，种子根和侧根的细胞死亡频率无显著差异。通过与经典隧道隧道法的比较，讨论了改进隧道隧道法的技术优势。
期刊介绍：
Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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