Content of Endogenous Phytohormones and Isoenzymes of Peroxidase in Male and Female Pistacia chinensis Plants Bunge Leaves

Abstract

To reveal the physiology differences in male and female plants of Pistacia chinensis Bunge,the contents of gibberellin(GA3),abscisic acid(ABA),indole-3-aceticacid(IAA),spermidine(Spd),spermine(Spm),putrescine(Put) and peroxidase isoenzyme in the leaves and petioles of male and female plants were determined.The contents of GA3 in female and male plants reached maximum values of 100.9 μg/g FW and 92.9 μg/g FW,respectively,on October 8,and showed very significant differences from June to September.The leaf ABA contents in male plants were higher than that in female plants,and showed very significant differences on May 21 and July 8,and reached minimum values of 222.0 ng/g FW and 340.1 ng/g FW,respectively,on May 21.The leaf IAA contents in male plants were higher than that in female plants,with the contents in female and male maximum values of 984.8 ng/g FW and 1000.6 ng/g FW,respectively,reached on September 8,and very significant differences exhibited on August 8.The ratios of leaf GA3/ABA in female plants were very significantly higher than that in male plants from July to September,and reached a maximum difference value of 42.8 on September 8.The maximum difference value of leaf IAA/ABA in male and female plants was 0.54 on May 21.The contents of Spd in female and male plants reached maximum values of 47.9 μg/g FW and 42.8 μg/g FW,respectively,in July,and there existed significant or very significant differences.The leaf Spm contents in male plants were higher than that in female plants,showing very significant differences on October 8 and reaching maximum values of 24.3 μg/g FW and 34.5 μg/g FW,respectively.The leaf Put contents in male plants were lower than that in female plants,with significant or very significant differences observed(except on May 21),and maximum values of 156.0 μg/g FW and 135.1 μg/g FW,respectively,on September 8.Significant differences were observed in peroxidase isoenzymes among male and female leaves and petioles.The peroxidase isoenzyme bands in female plant leaves were POD-1,POD-2,POD-3,POD-4,and POD-5,but the male plants bands were POD-2,POD-3,POD-4,and POD-5.The peroxidase isoenzyme bands in female plant petioles were POD-1',POD-3',POD-4',and POD-5',but the male plant bands were POD-2',POD-4',and POD-5'.The POD-1 was the endemic band in female plants leaves;POD-1' and POD-3' were the endemic bands in female plant petioles,and POD-2' was the endemic band in male plant petioles.From the above trial,we obtained the conclusions that the leaf ABA,IAA,Spd,Spm and Put contents were related to P.chinensis Bunge sex,and the peroxidase isoenzyme bands in leaves and petioles were the efficiency index to identify sex of P.chinensis Bunge.