Screening of Drought- and Salinity-responsive EST-SSR Markers inMedicago ruthenicaTrautv.

Plant Science Journal Pub Date : 2013-01-01 DOI:10.3724/SP.J.1142.2013.50493

Jie Liu, Die Hu, Hai-jia Chu, Juan Yan, Jian-qiang Li

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引用次数: 1

Abstract

To provide genetic markers for the study of ecological adaptability to responsive drought ̄ and salinity ̄stress for Medicago ruthenica Trautv.ꎬ we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought ̄and salinity ̄responsive EST ̄ SSR markers published for chickpea. DNA sequences were amplified with eight pairs of SSR primers by SSR ̄PCR technique. One hundred and eleven alleles were detectedꎬ on averageꎬ 13􀆰 88 alleles were detected per site. The mean values of observed heterozygosity and expected heterozygosity were 0􀆰 497 and 0􀆰 687ꎬ respectively. Polymorphism information content value ranged from 0􀆰 313 to 0􀆰 883ꎬ and the mean value was 0􀆰 649. These results indicate that the eight EST ̄SSR markers can be used to estimate genetic diversity of M. ruthenicaꎬ and genetic diversity was at a high level. Polymorphism EST ̄SSR markers can be used to investigate ecological adaptive evolution. The results contribute to revealing the genetic variation of drought ̄ and salt ̄tolerance genotypes and understanding the distribution pattern and ecological adaptation mechanism of M. ruthenica populations.

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紫花苜蓿抗旱、耐盐EST-SSR标记的筛选。

为研究紫花苜蓿对干旱和盐胁迫的生态适应性提供遗传标记。ꎬ从已发表的70对鹰嘴豆干旱和盐度EST - SSR标记中，筛选出多态性高、稳定性好的8对引物。利用SSR - PCR技术扩增8对SSR引物的DNA序列。平均每个位点检测到111个等位基因ꎬꎬ13􀆰88个等位基因。观察杂合度均值为0􀆰497，期望杂合度均值为0􀆰687ꎬ。多态性信息含量值范围为0􀆰313 ~ 0􀆰883ꎬ，平均值为0􀆰649。这些结果表明，8个EST - SSR标记可用于鲁塞尼卡m.a ruthenicaꎬ的遗传多样性估计，且遗传多样性处于较高水平。多态性EST / SSR标记可用于研究生态适应进化。研究结果有助于揭示麻豆耐干旱和耐盐基因型的遗传变异，了解麻豆种群的分布格局和生态适应机制。

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Plant Science Journal

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